Combined effects of nitrification inhibitor and zeolite on greenhouse gas fluxes and corn growth

  • Oslan JumadiEmail author
  • Yusminah Hala
  • R. Neni Iriany
  • Andi Takdir Makkulawu
  • Junja Baba
  • Hartono
  • St. Fatmah Hiola
  • Kazuyuki Inubushi
Research Article


Field and incubation experiments were conducted to determine the emission rate of greenhouse gases, nitrogen change, populations of AOB, NOB, and fungi as well as growth of corn in response to amendment of urea granulated with and without nitrification inhibitors and zeolite. The application of urea with neem, urea with zeolite, urea with zeolite + neem, urea with zeolite + dicyandiamide, and urea with dicyandiamide (UD) decreased the N2O emissions by 16.3%, 59.6%, 66.8%, 81.9%, 16.3%, and 86.7%, respectively. Meanwhile, patterns of CH4 fluxes were mostly determined by small emissions. Increase in corn height, weight of cobs, biomass, and chlorophyll leaf contents were not significantly different between urea alone and urea with NIs and zeolite. In the incubation experiment, the highest concentration of NH4+ and N2O production was detected during the first week and it remained high up to the second week of incubation in the combination of urea with NIs and zeolite treatments, although there was no significant difference compared with urea. During NH4+ decrease, the concentration of NO3 started to accumulate from the second to the third weeks. Production of CO2 showed no significant differences among treatments. The static production of CO2 could also explain that NIs and zeolite additions did not change AOB, NOB, and fungi activities after the fourth week of incubation.


Emission of N2O and CH4 CO2 Production Dicyandiamide Neem Nitrification inhibitor Zeolite 



The authors are very thankful to Indra Pramana, Muh. Dwi Prasetyo, Nurul Mutmainnah, and Ratna Dewi from the Biology Department, Universitas Negeri Makassar.

Funding information

This work was funded by The Ministry of Research, Technology and Higher Education of Indonesia under grant Penelitian Dasar and Penelitian Terapan.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Biology Department, Faculty of Mathematics and Natural SciencesUniversitas Negeri MakassarMakassarIndonesia
  2. 2.Plant BreedingCereals Research InstituteMarosIndonesia
  3. 3.Graduate School of HorticultureChiba UniversityChibaJapan

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