Advertisement

Differences in root surface adsorption, root uptake, subcellular distribution, and chemical forms of Cd between low- and high-Cd-accumulating wheat cultivars

  • Ya-Tao Xiao
  • Zhen-Jie Du
  • Carlos-A Busso
  • Xue-Bin QiEmail author
  • Hai-Qing Wu
  • Wei Guo
  • Da-Fu Wu
Research Article
  • 58 Downloads

Abstract

The differences in the mechanism of cadmium (Cd) accumulation in the grains of different wheat (Triticum aestivum L.) cultivars remain unclear. Thus, we conducted a hydroponic experiment in a greenhouse to compare root surface adsorption, root uptake, subcellular distribution, and chemical forms of Cd between low- and high-Cd-accumulating wheat cultivars at seedling stage, to improve our understanding of the differences between cultivars. The results showed that Cd adsorbed on the root surface was mainly in a complexed form, and the total amount of Cd on the Yaomai16 (YM, high-Cd-accumulating genotypes) root surface was higher (p < 0.05) than that on Xinmai9817 (XM, low-Cd-accumulating genotypes). A large amount of Cd ions adsorbed on root surface would cause plant damage and inhibit growth. Comparing the root-to-shoot translocation factors of Cd, the transfer coefficients of YM were 1.017, 1.446, 1.464, and 1.030 times higher than those of XM under 5, 10, 50, and 100 μmol L−1 Cd treatments, respectively. The subcellular distribution of Cd under Cd exposure is mainly in the cell wall and soluble fraction. The proportions of Cd in YM shoot soluble fraction were higher than those in XM, which was the main detoxification mechanism limiting the activity of Cd and may be responsible for low Cd accumulation in grains, while the effects of the chemical forms of Cd on migration and detoxification were not found to be related to Cd accumulation in the kernels.

Keywords

Winter wheat Cadmium Adsorption Subcellular distribution Chemical forms 

Notes

Acknowledgments

The author would like to extend their sincere gratitude to the Agriculture Water and Soil Environment Field Science Research Station, China, for the permission to carry out the research.

Author contributions

Y.-T.X. performed the experiments and drafted the manuscript. Z.-J.D. and X.-B.Q. provided some guidance in the experiments, and H.-Q.W., W.G., and D.-F.W. have contributed in the analysis of the data and further modifications of the manuscript. All authors read and approved the final version of the manuscript.

Funding information

Financial support for this research was provided by National Natural Science Foundation of China (Grand No.51779260, 51679241, 51709265), the National Key Research and Development Program of China (2016YFD0800703), and the Central Public-interest Scientific Institution Basal Research Fund (Farmland Irrigation Research Institute, CAAS, FIRI2017-13).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11356_2019_6708_MOESM1_ESM.docx (15 kb)
Table S1 (DOCX 14 kb)
11356_2019_6708_MOESM2_ESM.docx (6.4 mb)
Fig. S1 (DOCX 6594 kb)
11356_2019_6708_MOESM3_ESM.docx (5 mb)
Fig. S2 (DOCX 5079 kb)

References

  1. Ali H, Khan E, Sajad MA (2013) Phytoremediation of heavy metals--concepts and applications. Chemosphere 91:869–881CrossRefGoogle Scholar
  2. Bah AM, Sun H, Chen F, Zhou J, Dai H, Zhang G, Wu F (2010) Comparative proteomic analysis of Typha angustifolia leaf under chromium, cadmium and lead stress. J Hazard Mater 184:191–203CrossRefGoogle Scholar
  3. Bhatia NP, Walsh KB, Baker AJM (2005) Detection and quantification of ligands involved in nickel detoxification in a herbaceous Ni hyperaccumulator Stackhousia tryonii Bailey. J Exp Bot 56:1343–1349CrossRefGoogle Scholar
  4. Blum A, Sullivan CY (1997) The effect of plant size on wheat response to agents of drought stress. 1. Root drying. Aust J Plant Physiol 24:35–41Google Scholar
  5. Dalla Vecchia F, Rocca NL, Moro I, De Faveri S, Andreoli C, Rascio N (2005) Morphogenetic, ultrastructural and physiological damages suffered by submerged leaves of Elodea canadensis exposed to cadmium. Plant Sci 168:329–338CrossRefGoogle Scholar
  6. Dias MC, Monteiro C, Moutinho-Pereira J, Correia C, Gonçalves B, Santos C (2013) Cadmium toxicity affects photosynthesis and plant growth at different levels. Acta Physiol Plant 35:1281–1289CrossRefGoogle Scholar
  7. Fu XP, Dou CM, Chen YX, Chen XC, Shi JY, Yu MG, Xu J (2011) Subcellular distribution and chemical forms of cadmium in Phytolacca americana L. J Hazard Mater 186:103–107CrossRefGoogle Scholar
  8. Gouia H, Habib Ghorbal M, Meyer C (2000) Effects of cadmium on activity of nitrate reductase and on other enzymes of the nitrate assimilation pathway in bean. Plant Physiol Biochem 38:629–638CrossRefGoogle Scholar
  9. Greger M, Landberg T (2008) Role of rhizosphere mechanisms in Cd uptake by various wheat cultivars. Plant Soil 312:195–205CrossRefGoogle Scholar
  10. Hao XQ, Li TX, Yu HY, Zhang XZ, Zheng ZC, Chen GD, Zhang SJ, Zhao L, Pu Y (2015) Cd accumulation and subcellular distribution in two ecotypes of Kyllinga brevifolia Rottb as affected by Cd treatments. Environ Sci Pollut Res 22:7461–7469CrossRefGoogle Scholar
  11. Hart JJ, Welch RM, Norvell WA, Sullivan LA, Kochian LV (1998) Characterization of cadmium binding, uptake, and translocation in intact seedlings of bread and durum wheat cultivars. Plant Physiol 116:1413–1420CrossRefGoogle Scholar
  12. Haynes RJ (1980) Ion exchange properties of roots and ionic interactions within the root apoplasm: their role in ion accumulation by plants. Bot Rev 46:75–99CrossRefGoogle Scholar
  13. Hossain Z, Hajika M, Komatsu S (2012) Comparative proteome analysis of high and low cadmium accumulating soybeans under cadmium stress. Amino Acids 43:2393–2416CrossRefGoogle Scholar
  14. Kalis EJJ, Temminghoff EJM, Visser A, van Riemsdijk WH (2007) Metal uptake by Lolium perenne in contaminated soils using a four-step approach. Environ Toxicol Chem 26:335–345CrossRefGoogle Scholar
  15. Khan NA, Singh S, Nazar R (2007) Activities of antioxidative enzymes, sulphur assimilation, photosynthetic activity and growth of wheat (Triticum aestivum) cultivars differing in yield potential under cadmium stress. J Agron Crop Sci 193:435–444CrossRefGoogle Scholar
  16. Li Z, Tang S, Deng X, Wang R, Song Z (2010) Contrasting effects of elevated CO2 on Cu and Cd uptake by different rice varieties grown on contaminated soils with two levels of metals: implication for phytoextraction and food safety. J Hazard Mater 177(1-3):352–361CrossRefGoogle Scholar
  17. Lima AIG, Pereira SIA, de Almeida Paula Figueira EM, Caldeira GCN, de Matos Caldeira HDQ (2006) Cadmium detoxification in roots of Pisum sativum seedlings: relationship between toxicity levels, thiol pool alterations and growth. Environ Exp Bot 55:149–162CrossRefGoogle Scholar
  18. Lin RH, Lee CH, Chen WK, Lin-Shiau SY (1994) Studies on cytotoxic and genotoxic effects of cadmium nitrate and lead nitrate in Chinese hamster ovary cells. Environ Mol Mutagen 23:143–149CrossRefGoogle Scholar
  19. Liu Y, Xu RK (2015) The forms and distribution of aluminum adsorbed onto maize and soybean roots. J Soils Sediments 15:491–502CrossRefGoogle Scholar
  20. Liu W, Liang L, Zhang X, Zhou Q (2015) Cultivar variations in cadmium and lead accumulation and distribution among 30 wheat (Triticum aestivum L.) cultivars. Environ Sci Pollut Res 22:8432–8441CrossRefGoogle Scholar
  21. Liu ZD, Zhou Q, Hong ZN, Xu RK (2017) Effects of surface charge and functional groups on the adsorption and binding forms of Cu and Cd on roots of indica and japonica rice cultivars. Front Plant Sci 8:9Google Scholar
  22. Siu ER, Mruk DD, Porto CS, Cheng CY (2009) Cadmium-induced testicular injury. Toxicol Appl Pharmacol 238:240–249CrossRefGoogle Scholar
  23. Uraguchi S, Mori S, Kuramata M, Kawasaki A, Arao T, Ishikawa S (2009) Root-to-shoot Cd translocation via the xylem is the major process determining shoot and grain cadmium accumulation in rice. J Exp Bot 60:2677–2688CrossRefGoogle Scholar
  24. Wang X, Liu YO, Zeng GM, Chai LY, Song XC, Min ZY, Xiao X (2008) Subcellular distribution and chemical forms of cadmium in Bechmeria nivea (L.) Gaud. Environ Exp Bot 62:389–395CrossRefGoogle Scholar
  25. Wang ZW, Nan ZR, Wang SL, Zhao ZJ (2011) Accumulation and distribution of cadmium and lead in wheat (Triticum aestivum L.) grown in contaminated soils from the oasis, north-west China. J Sci Food Agric 91:377–384CrossRefGoogle Scholar
  26. Wang F, Wang M, Liu Z, Shi Y, Han T, Ye Y, Gong N, Sun J, Zhu C (2015a) Different responses of low grain-Cd-accumulating and high grain-Cd-accumulating rice cultivars to Cd stress. Plant Physiol Biochem 96:261–269CrossRefGoogle Scholar
  27. Wang JB, Su LY, Yang JZ, Yuan JG, Yin AG, Qiu Q, Zhang K, Yang ZY (2015b) Comparisons of cadmium subcellular distribution and chemical forms between low-Cd and high-Cd accumulation genotypes of watercress (Nasturtium officinale L. R. Br.). Plant Soil 396:325–337CrossRefGoogle Scholar
  28. Wang P, Deng XJ, Huang YA, Fang XL, Zhang J, Wan HB, Yang CY (2015c) Comparison of subcellular distribution and chemical forms of cadmium among four soybean cultivars at young seedlings. Environ Sci Pollut Res 22:19584–19595CrossRefGoogle Scholar
  29. Weigel HJ, Jager HJ (1980) Subcellular distribution and chemical form of cadmium in bean plants. Plant Physiol 65:480–482CrossRefGoogle Scholar
  30. Weng BS, Xie XY, Weiss DJ, Liu JC, Lu HL, Yan CL (2012) Kandelia obovata (S., L.) Yong tolerance mechanisms to cadmium: subcellular distribution, chemical forms and thiol pools. Mar Pollut Bull 64:2453–2460CrossRefGoogle Scholar
  31. Wu FB, Dong J, Qian QQ, Zhang GP (2005) Subcellular distribution and chemical form of Cd and Cd-Zn interaction in different barley genotypes. Chemosphere 60:1437–1446CrossRefGoogle Scholar
  32. Xin J, Huang B (2013) Subcellular distribution and chemical forms of cadmium in two hot pepper cultivars differing in cadmium accumulation. J Agric Food Chem 62:508–515CrossRefGoogle Scholar
  33. Xin J, Zhao X, Tan Q, Sun X, Hu C (2017) Comparison of cadmium absorption, translocation, subcellular distribution and chemical forms between two radish cultivars (Raphanus sativus L.). Ecotoxicol Environ Saf 145:258–265CrossRefGoogle Scholar
  34. Xue M, Zhou YH, Yang ZY, Lin BY, Yuan JG, Wu SS (2014) Comparisons in subcellular and biochemical behaviors of cadmium between low-Cd and high-Cd accumulation cultivars of pakchoi (Brassica chinensis L.). Front Environ Sci Eng 8:226–238CrossRefGoogle Scholar
  35. Zhang Y, Yang X (1994) The toxic effects of cadmium on cell division and chromosomal morphology of Hordeum vulgare. Mutat Res Environ Mutagen Relat Subj 312:121–126Google Scholar
  36. Zhao YF, Wu JF, Shang DR, Ning JS, Zhai YX, Sheng XF, Ding HY (2015) Subcellular distribution and chemical forms of cadmium in the edible seaweed, Porphyra yezoensis. Food Chem 168:48–54CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Ya-Tao Xiao
    • 1
    • 2
    • 3
  • Zhen-Jie Du
    • 1
    • 2
  • Carlos-A Busso
    • 4
  • Xue-Bin Qi
    • 1
    Email author
  • Hai-Qing Wu
    • 1
    • 2
  • Wei Guo
    • 1
    • 2
  • Da-Fu Wu
    • 5
  1. 1.Farmland Irrigation Research InstituteChinese Academy of Agricultural SciencesXinxiangPeople’s Republic of China
  2. 2.Key Laboratory of High-Efficient and Safe Utilization of Agriculture Water Resources of CAASXinxiangPeople’s Republic of China
  3. 3.Graduate University of Chinese Academy of Agricultural SciencesBeijingPeople’s Republic of China
  4. 4.Departamento de Agronomía-CERZOS (CONICET)Universidad Nacional del SurBahía BlancaArgentina
  5. 5.College of Resources and EnvironmentHenan Institute of Science and TechnologyXinxiangPeople’s Republic of China

Personalised recommendations