Biochar suppresses N2O emissions and alters microbial communities in an acidic tea soil
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Biochar has been considered as a promising soil amendment for improving fertility and mitigating N2O emission from the arable land. However, biochar’s effectiveness in acidic tea soil and underlying mechanisms are largely unknown. We conducted a short-term microcosm experiment using two biochars (1% w/w, LB, generated from legume and NLB, non-legume biomass, respectively) to investigate the effects of biochar amendments on soil chemical properties, N2O emission, and microbial community in an acidic soil. Soil and headspace gas samples were taken on 1, 10, and 30 day’s incubation. Biochar amendment increased soil pH and DOC, however, significantly reduced soil inorganic N. Both biochars at ~ 1% addition had little effect on microbial CO2 respiration but suppressed soil N2O emission by ~ 40% during the incubation. The divergence in N2O efflux rates between soils with and without biochar addition aligned to some degree with changes in soil pH, inorganic N, and dissolved organic C (DOC). We also found that biochar addition significantly modified the fungal community structure, in particular the relative abundance of members of Ascomycota, but not the bacterial community. Furthermore, the copy number of nosZ, the gene encoding N2O reductase, was significantly greater in biochar-amended soils than the soil alone. Our findings contribute to better understanding of the impact of biochar on the soil chemical properties, soil N2O emission, and microbial community and the consequences of soil biochar amendment for improving the health of acidic tea soil.
KeywordsBiochar N2O emission Tea plantation Microbial community Acidic soil Denitrification
This work was supported by the National Natural Science Foundation of China (Grant Nos. 41525002, 41701282, and 41761134085), the Strategic Priority Program of the Chinese Academy of Science (Grant No. XDB15020300), the National Ten-Thousand Talents Program of China (201829), and the Two-Hundred Talents Plan of Fujian Province, China (2018A12).
- Chen J, Liu X, Zheng J, Zhang B, Lu H, Chi Z, Pan G, Li L, Zheng J, Zhang X, Wang J, Yu X (2013). Biochar soil amendment increased bacterial but decreased fungal gene abundance with shifts in community structure in a slightly acid rice paddy from Southwest China. Appl Soil Ecol 71: 33-44. https://doi.org/10.1016/j.apsoil.2013.05.003 CrossRefGoogle Scholar
- Domingues RR, Trugilho PF, Silva CA, Melo d, Isabel Cristina NA, Melo LC, Magriotis ZM, Sánchez-Monedero MA (2017, e0176884) Properties of biochar derived from wood and high-nutrient biomasses with the aim of agronomic and environmental benefits. PLoS One 12. https://doi.org/10.1371/journal.pone.0176884 CrossRefGoogle Scholar
- Jenkins JR, Viger M, Arnold EC, Harris ZM, Ventura M, Miglietta F, Girardin C, Edwards RJ, Rumpel C, Fornasier F, Zavalloni C (2017) Biochar alters the soil microbiome and soil function: results of next-generation amplicon sequencing across Europe. GCB Bioenergy 9(3):591–612. https://doi.org/10.1111/gcbb.12371 CrossRefGoogle Scholar
- Khan S, Waqas M, Ding F, Shamshad I, Arp HP, Li G (2015) The influence of various biochars on the bioaccessibility and bioaccumulation of PAHs and potentially toxic elements to turnips (Brassica rapa L.). J Hazard Mater 300:243–253. https://doi.org/10.1016/j.jhazmat.2015.06.050 CrossRefGoogle Scholar
- Sánchez-García M, Roig A, Sánchez-Monedero MA, &CayuelaML (2014) Biochar increases soil N2O emissions produced by nitrification-mediated pathways. Front Env Sci 2:25. https://doi.org/10.3389/fenvs.2014.00025
- Schirrmann M, Cayuela ML, Fuertes-Mendizábal T, Estavillo JM, Ippolito J, Spokas K, Novak J, Kammann C, Wrage-Mönnig N, Borchard N(April, 2017) Biochar reduces N2O emissions from soils: a meta-analysis. In EGU General Assembly Conference Abstracts (Vol. 19, p. 8265).Google Scholar
- USEPA (2008) Registering pesticides. Washington D.C., pp 11–26Google Scholar