Effects of low-dosage ozone pre-treatment on the anaerobic digestion of secondary and mixed sludge

  • Agostina ChiavolaEmail author
  • Emilio D’Amato
  • Maria Rosaria Boni
Research Article


The present study investigated the effects of ozonation pre-treatment at low-ozone dosage (below 100 mgO3/gTS0) with respect to previous studies, on the anaerobic digestion of waste-activated sludge alone and a mixture of activated sludge and primary sludge. Methane production and volatile suspended solids reduction efficiency were determined for different specific ozone dosages and compared with the values obtained in the absence of pre-treatment. Among the dosages tested in the study (from 4.8 to 73.2 mgO3/gTS0 for mixed sludge and from 3.5 to 53.6 mgO3/gTS0 for waste-activated sludge), the best results were obtained at the lowest ones: 4.8 and 3.5 mgO3/gTS0 for mixed sludge and waste-activated sludge, respectively. Indeed, at this dosage, an additional methane production of about 6% and 30% was achieved for mixed and waste-activated sludge, respectively; furthermore, the maximum CH4 production rate increased of about 21% and 33% for mixed and waste-activated sludge, respectively. With respect to the Gompertz model, the modified logistic model provided the best agreement to the experimental data of the specific methane yield production. The present study demonstrated the importance of investigating the application of low dosages when ozonation is being evaluated as a pre-treatment to enhance anaerobic digestion performance.


Activated sludge Anaerobic process Methane Ozonation Volatile solids 



  1. APHA (2005) Standard methods for the examination of water and wastewater, 20th edn. American Public Health Association, Washington, DCGoogle Scholar
  2. Bougrier C, Albasi C, Delgenès JP, Carrère H (2006) Effect of ultrasonic, thermal and ozone pre-treatments on waste activated sludge solubilisation and anaerobic biodegradability. Chem Eng Process: Process Intensif. 45(8):711–718CrossRefGoogle Scholar
  3. Bougrier C, Battimelli A, Delgenes JP, Carrere H (2007) Combined ozone pretreatment and anaerobic digestion for the reduction of biological sludge production in wastewater treatment. Ozone-SciEng 29(3):201–206CrossRefGoogle Scholar
  4. Carlsson M, Lagerkvist A, Morgan-Sagastume F (2012) The effects of substrate pre-treatment on anaerobic digestion systems: A review. Waste Manag 32:1634–1650CrossRefGoogle Scholar
  5. Carrère H, Dumas C, Battimelli A, Batstone DJ, Delgenes JP, Steyer JP, Ferrer I (2010) Pretreatment methods to improve sludge anaerobic degradability: a review. J Hazard Mater 183:1–15CrossRefGoogle Scholar
  6. Chacana J, Alizadeh S, Labelle M-A, Laporte A, Hawari J, Barbeau B, Comeau Y (2017) Effect of ozonation on anaerobic digestion sludge activity and viability. Chemosphere 176:405–411CrossRefGoogle Scholar
  7. Chiavola A, D’Amato E, Gori R, Lubello C, Sirini P (2013) Techno-economic evaluation of the application of ozone-oxidation in a full-scale aerobic digestion plant. Chemosphere 91:656–662CrossRefGoogle Scholar
  8. Chu L, Yan S, Xing X-H, Sun X, Jurcik B (2009) Progress and perspectives of sludge ozonation as a powerful pretreatment method for minimization of excess sludge production. Water Res 43:1811–1822CrossRefGoogle Scholar
  9. Dhar BR, Nakhla G, Ray MB (2012) Techno-economic evaluation of ultrasound and thermal pretreatments for enhanced anaerobic digestion of municipal waste activated sludge. Waste Manage 32(3):542–549CrossRefGoogle Scholar
  10. Fall C, Silva-Hernández BC, Hooijmans CM, Lopez-Vazquez CM, Esparza-Soto M, Lucero-Chávez M, van Loosdrecht MCM (2018) Sludge reduction by ozone: Insights and modeling of the dose-response effects. J Environ Manag 206:103–112CrossRefGoogle Scholar
  11. Feki E, Khoufi S, Loukil S, Sayadi S (2015) Improvement of anaerobic digestion of waste-activated sludge by using H2O2 oxidation, electrolysis, electro-oxidation and thermo-alkaline pretreatments. Environ Sci Pollut Res 22:14717–14726CrossRefGoogle Scholar
  12. Gao Y, Duan Y, Fan W, Guo T, Huo M, Yang W, Zhu S, An W (2019) Intensifying ozonation treatment of municipal secondary effluent using a combination of microbubbles and ultraviolet irradiation. Environ Sci Pollut Res 26:21915–21924CrossRefGoogle Scholar
  13. Goel R, Tokutomi T, Yasui H, Noike T (2003) Optimal process configuration for anaerobic digestion with ozonation. Water Sci Technol 48(4):85–96CrossRefGoogle Scholar
  14. Meng X, Liu D, Frigon M (2015) The process of activated sludge ozonation: effect of ozone on cells, flocs, macromolecules and nutrient release. Water Sci Technol 71:1026–1032CrossRefGoogle Scholar
  15. Metcalf & Eddy (2003) Wastewater Engineering. McGraw-Hill, New YorkGoogle Scholar
  16. Ӧzӧn E, Erdinçler A (2019) Effects of microwave, H2O2/MW and H2O2/heat pre-treatments on the methane production from wastewater sludges: experimental and modeling approach. Environ Sci Pollut Res Int. Published on line 20 May 2019
  17. Park KY, Maeng SK, Song KG, Ahn KH (2008) Ozone treatment of wastewater sludge for reduction and stabilization. J Environ Sci Health Part A 43:1546–1550CrossRefGoogle Scholar
  18. Parkin G, Owen WF (1986) Fundamentals of anaerobic digestion of wastewater sludges. J Environ Eng 112(5):867–920CrossRefGoogle Scholar
  19. Pei J, Yao H, Wang H, Shan D, Jiang Y, Ma L, Yu X (2015) Effect of ultrasonic and ozone pre-treatments on pharmaceutical waste activated sludge's solubilization, reduction, anaerobic biodegradability and acute biological toxicity. Bioresour Technol 192:418–423CrossRefGoogle Scholar
  20. Pei J, Yao H, Wang H, Ren J, Yu X (2016) Comparison of ozone and thermal hydrolysis combined with anaerobic digestion for municipal and pharmaceutical waste sludge with tetracycline resistance genes. Water Res 99:122–128CrossRefGoogle Scholar
  21. Pérez-Elvira S, Fdz-Polanco M, Plaza FI, Garralón G, Fdz-Polanco F (2009) Ultrasound pre-treatment for anaerobic digestion improvement. Water Sci Technol 60(6):1525–1532CrossRefGoogle Scholar
  22. Qiang Z, Nie Y, Ben W, Qu J, Zhang H (2013) Degradation of endocrine disrupting chemicals during activated sludge reduction by ozone. Chemosphere 91:366–373CrossRefGoogle Scholar
  23. Qiang Z, Wang L, Dong H, Qu J (2015) Operation performance of an A/A/O process coupled with excess sludge ozonation and phosphorus recovery: a pilot-scale study. Chem Eng J 268:162–169CrossRefGoogle Scholar
  24. Salsabil MR, Laurent J, Casellas M, Dagot C (2010) Techno-economic evaluation of thermal treatment, ozonation and sonication for reduction of wastewater biomass volume before aerobic or anaerobic digestion. J Hazard Mater 174:323–333CrossRefGoogle Scholar
  25. Tian X, Wang C, Trzcinski AP, Lin L, Ng WJ (2015) Interpreting the synergistic effect in combined ultrasonication–ozonation sewage sludge pre-treatment. Chemosphere 140:63–71CrossRefGoogle Scholar
  26. Vlyssides AG, Karlis PK (2004) Thermal-alkaline solubilization of waste activated sludge as a pre-treatment stage for anaerobic digestion. Bioresour Technol 91(2):201–206CrossRefGoogle Scholar
  27. Weemaes M, Grootaerd H, Simoens F, Verstraete W (2000) Anaerobic digestion of ozonized biosolids. Water Res 34(8):2330–2336CrossRefGoogle Scholar
  28. Wenjing L, Chao P, Lama A, Xindi F, Rong Y, Dhar BP (2019) Effect of pre-treatments on biological methane potential of dewatered sewage sludge under dry anaerobic digestion. Ultrasonics-Sonochem 52:224–231CrossRefGoogle Scholar
  29. Xu G, Chen S, Shi J, Wang S, Zhu G (2010) Combination treatment of ultrasound and ozone for improving solubilisation and anaerobic biodegradability of waste activated sludge. J Hazard Mate 180:340–346CrossRefGoogle Scholar
  30. Yan ST, Chu LB, Xing XH, Yu AF, Sun XL, Jurcik B (2009) Analysis of the mechanism of sludge ozonation by a combination of biological and chemical approaches. Water Res 43:195–203CrossRefGoogle Scholar
  31. Yang S-S, Guo W-Q, Cao G-L, Zheng H-S, Ren NQ (2012) Simultaneous waste activated sludge disintegration and biological hydrogen production using an ozone/ultrasound pretreatment. Bioresour Technol 124:347–354CrossRefGoogle Scholar
  32. Yasui H, Shibata M (1994) An innovative approach to reduce excess sludge production in the activated sludge process. Water Sci Technol 30(9):11–20CrossRefGoogle Scholar
  33. Yasui H, Nakamura K, Sakuma S, Iwasaki M, Sakai Y (1996) A full-scale operation of a novel activated sludge process without excess sludge production. Water Sci Technol 34(3–4):395–404CrossRefGoogle Scholar
  34. Zhang G, Yang J, Liu H, Zhang J (2009) Sludge ozonation: disintegration, supernatant changes and mechanisms. Bioresour Technol 100:1505–1509CrossRefGoogle Scholar
  35. Zwietering MH, Jongenburger I, Rombouts FM, Van ’t Riet K (1990) Modeling of the bacterial growth curve. Appl Environ Microbiol 56:1875–1881Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Civil, Constructional and Environmental Engineering, Faculty of Civil and Industrial EngineeringSapienza University of RomeRomeItaly

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