Protective effects of hesperidin and diosmin against acrylamide-induced liver, kidney, and brain oxidative damage in rats

  • Abdelazim E. Elhelaly
  • Gadah AlBasher
  • Saleh Alfarraj
  • Rafa Almeer
  • Eshak I. Bahbah
  • Maged M. A. Fouda
  • Simona G. Bungău
  • Lotfi Aleya
  • Mohamed M. Abdel-DaimEmail author
Research Article


Acrylamide (AA) is a heat-induced toxin formed during thermal processing of many commonly consumed foods, including meat products, French fries, potato crisps, bread, cereals, cookies, and coffee. There is thus potentially high dietary exposure of humans to AA, which can induce significant oxidative stress. Hesperidin (HS) and diosmin (DS) are flavone glycosides that have antioxidant properties. The aim of this study was to investigate the protective effects of HS and DS against AA toxicity. Fifty-six adult male Wistar albino rats were divided into seven groups. The first group was orally administered 0.5% (w/v) dimethyl sulfoxide (DMSO) and considered as the control group. The second and third groups were orally administered 10 mg/kg/day of HS or DS, respectively. The fourth group received 20 mg/kg/day of AA orally for 14 days. The fifth and sixth groups were given 10 mg/kg/day of HS or DS, respectively, followed by AA. The seventh group was given both HS and DS after AA administration. AA intoxication significantly (p ≤ 0.05) increased serum levels of liver function enzymes (ALT, AST, and ALP), kidney function products (urea and creatinine), oxidative DNA damage marker (OHdG), proinflammatory markers (TNF-α, IL-1β, and IL-6), lipid peroxidation marker (malondialdehyde), and nitric oxide (NO). On the other hand, it significantly (p ≤ 0.05) decreased levels of reduced glutathione (GSH) in the liver, kidney, and brain. The activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) in the liver, kidney, and brain tissues were also reduced. HS and DS supplementation prevented lipid peroxidation, normalized the serum parameters altered by AA, and enhanced the tissue concentrations and activities of antioxidant biomarkers. It could be concluded that HS and DS have potent protective effects against oxidative stress, lipid peroxidation, and DNA damage induced by AA toxicity in rats.


Acrylamide Antioxidant Flavone Hepatotoxicity Nephrotoxicity Neurotoxicity 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


This project was funded by Research supporting project number (RSP-2019/95) king Saud University,Riyadh,Saudi Arabia.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Abdelazim E. Elhelaly
    • 1
  • Gadah AlBasher
    • 2
  • Saleh Alfarraj
    • 2
  • Rafa Almeer
    • 2
  • Eshak I. Bahbah
    • 3
  • Maged M. A. Fouda
    • 4
    • 5
  • Simona G. Bungău
    • 6
  • Lotfi Aleya
    • 7
  • Mohamed M. Abdel-Daim
    • 2
    • 8
    Email author
  1. 1.Department of Food Hygiene and Control, Faculty of Veterinary MedicineSuez Canal UniversityIsmailiaEgypt
  2. 2.Department of Zoology, Science CollegeKing Saud UniversityRiyadhSaudi Arabia
  3. 3.Faculty of MedicineAl-Azhar UniversityDamiettaEgypt
  4. 4.Biology Department, College of ScienceJouf UniversitySakakaSaudi Arabia
  5. 5.Department of Zoology, Faculty of ScienceAl-Azhar University, Assuit BranchAssuitEgypt
  6. 6.Department of Pharmacy, Faculty of Medicine and PharmacyUniversity of OradeaOradeaRomania
  7. 7.Bourgogne Franche-Comté University, Chrono-Environnement Laboratory, UMR CNRS 6249Besançon CedexFrance
  8. 8.Pharmacology Department, Faculty of Veterinary MedicineSuez Canal UniversityIsmailiaEgypt

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