Diosmin ameliorative effects on oxidative stress and fibrosis in paraquat-induced lung injury in mice

  • Sirus Mirzaee
  • Esrafil Mansouri
  • Maryam Shirani
  • Marzieh Zeinvand-Lorestani
  • Mohammad Javad KhodayarEmail author
Research Article


Paraquat (PQ) induces pulmonary fibrosis, a progressive lung disorder resulting in severe respiratory failure and death. Increased oxidative stress, inflammatory reactions, and multiple fibrotic lesions are major features of PQ-induced lung injury. Diosmin (Dio) is a safe drug that is available for clinical use for vascular disorders. Dio exhibits antioxidant, anti-inflammatory, and antifibrotic activities. Accordingly, the aim of this study was to evaluate the protective effect of diosmin on PQ-induced lung injury in mice and the underlying mechanisms involved. Lung injury was induced by PQ (30 mg/kg, intraperitoneally) in NMRI albino mice and Dio (50 and 100 mg/kg, gavage) was administrated 3 days before PQ and continued for 10 or 24 days. After euthanizing the mice, the biochemical and histopathological markers of lung tissue were determined. PQ significantly increased oxidative stress, inflammatory, and fibrotic markers. PQ increased the level of malonedaldehyde (MDA) and hydroxyproline (HYP) and decreased the level of glutathione (GSH) and catalase activity in the lung. Dio (50 and 100 mg/kg) significantly increased GSH levels and catalase activity and decreased HYP content and MDA levels. In addition, Dio reduced histopathological injuries in hematoxylin and eosin–stained and Masson’s trichrome–stained sections. These findings suggest that Dio has protective effects against PQ-induced lung injury, which may be due to its antioxidant, anti-inflammatory, and antifibrotic effects.


Paraquat Pulmonary injury Diosmin Antioxidant Antifibrotic Mice 


Funding information

This paper is issued from the Pharm.D thesis of Sirus Mirzaee and was financially supported by the Toxicology Research Center (Grant Number: TRC-9607) of AJUMS.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Medicinal Plant Research CenterAhvaz Jundishapur University of Medical SciencesAhvazIran
  2. 2.Department of Anatomical Sciences, School of Medicine and Cellular and Molecular Research CenterAhvaz Jundishapur University of Medical SciencesAhvazIran
  3. 3.Toxicology Research CenterAhvaz Jundishapur University of Medical SciencesAhvazIran
  4. 4.Department of Toxicology, Faculty of PharmacyAhvaz Jundishapur University of Medical SciencesAhvazIran

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