Environmental Science and Pollution Research

, Volume 26, Issue 32, pp 33683–33693 | Cite as

Degradation mechanism of lignocellulose in dairy cattle manure with the addition of calcium oxide and superphosphate

  • Yingying Cai
  • Yanhua He
  • Kang He
  • Haijun Gao
  • Meijie Ren
  • Guangfei QuEmail author
Research Article


Cellulose and lignin belongs to refractory organic matters in the traditional composting. In this research, the degradation of lignocellulose in dairy cattle manure was investigated through adding calcium oxide (CaO) and superphosphate (SSP). In the presence of CaO and SSP, the degradation rate of cellulose and lignin were improved by 25.0% and 8.33%, respectively. The results indicated that the pH value in system would be slightly higher with the addition of CaO and SSP. Besides, the pH value of all cow manure piles were about 8.4 after composting rotten, which could be well neutralized by the gradually acidified soil in the southwest of China with the full effect of fertilizer released. In addition, the abundance of Bacillales, Actinomycetes, and Thermoactinomycetaceae in the experimental groups (AR) was slightly better than that in the control groups (CK) during composting, which led to a conclusion that an elaborate physical–chemical–multivariate aerobic microorganism evolution model of cellulose degradation products (PCMC) was deduced and the physical–chemical–multivariate aerobic microorganism model of lignin cycle degradation (PCML) was developed.


Calcium oxide Cellulose evolution model Lignin degradation model Microbial diversity Superphosphate 



The work was financially supported by the National Major Science and Technology projects (grant no. 2014ZX07105-001); the National Natural Science Foundation of China (no. 21377048).

Supplementary material

11356_2019_6444_MOESM1_ESM.docx (263 kb)
ESM 1 (DOCX 263 kb)


  1. Arias O, Viña S, Uzal M, Soto M (2017) Composting of pig manure and forest green waste amended with industrial sludge. Sci Total Environ 586:1228–1236CrossRefGoogle Scholar
  2. Babyranidevi S, Bhoyar RV (2003) Feasibility of some treatments for improving the composting of municipal solid waste. Indian J Environ Health 45(3):231–234Google Scholar
  3. Bernal MP, Alburquerque JA, Moral R (2009) Composting of animal manures and chemical criteria for compost maturity assessment. A review. Bioresour Technol 100(22):5444–5453CrossRefGoogle Scholar
  4. Bhattacharya A, Pletschke BI (2014) Thermophilic bacilli and their enzymes in composting. Springer International Publishing, Berlin, p 103–124Google Scholar
  5. Bohacz J (2016) Lignocellulose-degrading enzymes, free-radical transformations during composting of lignocellulosic waste and biothermal phases in small-scale reactors. Sci Total Environ 580:744–754CrossRefGoogle Scholar
  6. Bugg TDH, Ahmad M, Hardiman EM, Singh R (2010) The emerging role for bacteria in lignin degradation and bio-product formation. Curr Opin Biotechnol 22(3):394–400CrossRefGoogle Scholar
  7. Bustamante MA, Paredes C, Marhuenda-Egea FC, Pérez-Espinosa A, Bernal MP, Moral R (2008) Co-composting of distillery wastes with animal manures: carbon and nitrogen transformations in the evaluation of compost stability. Chemosphere 72(4):551–557CrossRefGoogle Scholar
  8. Butler TA, Sikora LJ, Steinhilber PM, Douglass LW (2001) Compost age and sample storage effects on maturity indicators of biosolids compost. J Environ Qual 30(6):2141–2148CrossRefGoogle Scholar
  9. Chu J, Ma L, Zhang J H (2016) The chemical composition of bamboo after heat pretreatment with Fourier infrared spectrum analysis. Spectrosc Soect Anal 36(11):3557–3562Google Scholar
  10. Fan B, Wang X (2011) Effect of temperature on the transformation of nitrogen in aerobic composting process for human feces disposal. Environ Chem 30(7):1266–1270Google Scholar
  11. Fan B A I, Xiaochang W (2011) Effect on biodegradation and nitrogen holding property from temperature during aerobic composting for sanitary disposal of human feces. Soil and Fertilizer Sciences in China (3):68–71Google Scholar
  12. Fan P, Tian J, Huang J, Lei W, Qiu H (2008) Methods for determination of cellulose and lignin in peanut shells. J Chongqing Inst Sci Technol 10(5):64–65Google Scholar
  13. Fan G, Liao C, Fang T, Wang M, Song G (2013a) Hydrolysis of cellulose catalyzed by sulfonated poly (styrene-co-divinylbenzene) in the ionic liquid 1-n-butyl-3-methylimidazolium bromide. Fuel Process Technol 116(6):142–148CrossRefGoogle Scholar
  14. Fan G, Liao C, Fang T, Wang M, Song G, Fan G, Liao C, Fang T, Song G (2013b) Hydrolysis of cellulose catalyzed by sulfonated poly(styrene-co-divinylbenzene) in the ionic liquid 1-n-butyl-3-methylimidazolium bromide. Fuel Process Technol 116(6):142–148CrossRefGoogle Scholar
  15. Földváry CM, Takács E, Wojnárovits L (2003) Effect of high-energy radiation and alkali treatment on the properties of cellulose. Radiat Phys Chem 67(3–4):505–508CrossRefGoogle Scholar
  16. Gardner DJ (1994) Essentials of pulping and papermaking. Bioresour Technol 47(2):191–192CrossRefGoogle Scholar
  17. Gusenkova AA, Pleshchinskii NB (2000) Integral equations with logarithmic singularities in the kernels of boundary-value problems of plane elasticity theory for regions with a defect. J Appl Math Mech 64(3):435–441CrossRefGoogle Scholar
  18. Himmel ME, Ding SY, Johnson DK, Adney WS, Nimlos MR, Brady JW, Foust TD (2007) Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science 315(5813):804–807CrossRefGoogle Scholar
  19. Humphrey AE (2010) The hydrolysis of cellulosic materials to useful products. J Appl Polym Sci 23(12):3525–3539Google Scholar
  20. Jiao YZ, Gao Z, Li G, Li PF, Li PP (2015) Effect of different indigenous microorganisms and its composite microbes on degradation of corn straw. Transac Chin Soc Agric Eng 3(1):14–36Google Scholar
  21. Jiaxi Z, Yuansong WEI, Xiaofeng WU, Xiaolan Z, Shenghui HAN, Yun F (2011) Nutrients conservation of N & P and greenhouse gas reduction of N2O emission during swine manure composting. Environ Sci 32(7):2047–2055Google Scholar
  22. Kirk TK, Shimada M (1985) Lignin biodegradation: the microorganisms involved and the physiology and biochemistry of degradation by white-rot fungi. Elsevier Inc., AmsterdamGoogle Scholar
  23. Külcü R (2015) Determination of the relationship between FAS values and energy consumption in the composting process. Ecol Eng 81:444–450CrossRefGoogle Scholar
  24. Liang DL, Jie G, Qin QJ, Hua G, Li SX (2009) Effects of inoculants on enzymes activities of pig manure during high temperature composting. Transac Chin Soc Agric Eng 25(9):243–248Google Scholar
  25. Liu K, Price GW (2011) Evaluation of three composting systems for the management of spent coffee grounds. Bioresour Technol 102(17):7966–7974CrossRefGoogle Scholar
  26. Liu N, Zhou J, Han L, Huang G (2017) Characterization of lignocellulosic compositions’ degradation during chicken manure composting with added biochar by phospholipid fatty acid (PLFA) and correlation analysis. Sci Total Environ 586:1003–1011CrossRefGoogle Scholar
  27. Mijaylova Nacheva P, Moeller G, Chavez RCE, Cardaso Vigueros L (2002) Characterization and dewaterability of raw and stabilized sludge using different treatment methods. Water Sci Technol 46(10):123–130CrossRefGoogle Scholar
  28. Nakasaki K, Marui T (2011) Progress of organic matter degradation and maturity of compost produced in a large-scale composting facility. Waste Manag Res J Int Solid Wastes Public Clean Assoc Iswa 29(6):574–581CrossRefGoogle Scholar
  29. Nakasaki K, Tran LTH, Idemoto Y, Abe M, Rollon AP (2009) Comparison of organic matter degradation and microbial community during thermophilic composting of two different types of anaerobic sludge. Bioresour Technol 100(2):676–682CrossRefGoogle Scholar
  30. Narita H, Zavala MAL, Iwai K, Ito R, Funamizu N (2005) Transformation and characterisation of dissolved organic matter during the thermophilic aerobic biodegradation of faeces. Water Res 39(19):4693–4704CrossRefGoogle Scholar
  31. Onwosi CO, Igbokwe VC, Odimba JN, Eke IE, Nwankwoala MO, Iroh IN, Ezeogu LI (2017) Composting technology in waste stabilization: on the methods, challenges and future prospects. J Environ Manag 190:140–157CrossRefGoogle Scholar
  32. Petric I, Avdihodžić E, Ibrić N (2015) Numerical simulation of composting process for mixture of organic fraction of municipal solid waste and poultry manure. Ecol Eng 75:242–249CrossRefGoogle Scholar
  33. Qian X, Shen G, Wang Z, Guo C, Liu Y, Lei Z, Zhang Z (2014) Co-composting of livestock manure with rice straw: characterization and establishment of maturity evaluation system. Waste Manag 34(2):530–535CrossRefGoogle Scholar
  34. Ren GM, Xu XH, Qu JJ, Zhu LP, Wang TT (2016) Evaluation of microbial population dynamics in the co-composting of cow manure and rice straw using high throughput sequencing analysis. World J Microbiol Biotechnol 32(6):1–11CrossRefGoogle Scholar
  35. Rynk R, Kamp MVD, Willson GB, Singley ME, Richard TL & Kolega JJ(1992) On-farm composting handbook. Applied engineering in agriculture (6):273–281Google Scholar
  36. Sanderson K (2011) Lignocellulose: a chewy problem. Nature 474(7352):S12–S14CrossRefGoogle Scholar
  37. Segal LC, Creely J, Martin AEJ, Conrad CM (1959) An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer. Text Res J 29(10):786–794CrossRefGoogle Scholar
  38. Segal LC, Creely J, Martin AEJ, Conrad CM (2000) An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer. Text Res J 29(10):786–794CrossRefGoogle Scholar
  39. Sharma VK, Canditelli M, Fortuna F, Cornacchia G (1997) Processing of urban and agro-industrial residues by aerobic composting: review. Energy Convers Manag 38(5):453–478CrossRefGoogle Scholar
  40. Široký J, Blackburn RS, Bechtold T, Taylor J, White P (2011) Alkali treatment of cellulose II fibres and effect on dye sorption. Carbohydr Polym 84(1):299–307CrossRefGoogle Scholar
  41. Tao L, Guo W-j, Fang L, Fang Z (2013) Comparison and analysis of cotton stalk cellulose crystallinity of 3 kinds of methods. J Northeast Forest Univ 41(2):89–92Google Scholar
  42. Tran TM, Luxhøi J, Jensen LS (2012) Turnover of manure N-labelled ammonium during composting and soil application as affected by lime and superphosphate addition. Soil Sci Soc Am J 77(1):190–201CrossRefGoogle Scholar
  43. Tubail K, Chen L, Jr FCM, Keener HM, Rigot JF, Klingman M, Kost D, Dick WA (2008) Gypsum additions reduce ammonia nitrogen losses during composting of dairy manure and biosolids. Compost Sci Util 16(4):285–293CrossRefGoogle Scholar
  44. Tuomela M, Hatakka A, Itavaara MVM (2000) Biodegradation of lignin in a compost environment: a review. Bioresour Technol 72(2):169–183CrossRefGoogle Scholar
  45. Vázquez MA, Varga DDL, Plana R, Soto M (2015) Integrating liquid fraction of pig manure in the composting process for nutrient recovery and water re-use. J Clean Prod 104:80–89CrossRefGoogle Scholar
  46. Wang P, Changa CM, Watson ME, Dick WA, Chen Y, Haj H (2004) Maturity indices for composted dairy and pig manures. Soil Biol Biochem 36(5):767–776CrossRefGoogle Scholar
  47. Wang H, Sheng S, Wang X, Han G (2011) Degradation characteristics of different organic components in human feces by composting reactor. China Water Wastewater 27(3):81–83Google Scholar
  48. Wang K, Li X, He C, Chen CL, Bai J, Ren N, Wang JY (2014a) Transformation of dissolved organic matters in swine, cow and chicken manures during composting. Bioresour Technol 168(3):222–228CrossRefGoogle Scholar
  49. Wang S, Liu K, Li R, Wang J, Jin Z, Yang J (2014b) Enhanced technology of cattle manure compost by microbial inoculum with high lignocellulose degradation ability. Transac Chin Soc Agric Mach 45(4):201–207Google Scholar
  50. Wei Y, Zhao Y, Xi B, Wei Z, Li X, Cao Z (2015) Changes in phosphorus fractions during organic wastes composting from different sources. Bioresour Technol 189:349–356CrossRefGoogle Scholar
  51. Wu CY, Li QF, Zhang YB (2011) Control of nitrogen loss during co-composting of banana stems with chicken manure. Adv Mater Res 295-297:773–776CrossRefGoogle Scholar
  52. Xi B (2002) Study on current status of lignin and cellulose biodegradation in composting process. Tech Equip Environ Poll Cont 3(3):19–23Google Scholar
  53. Xu J, Yang Q (2010) Isolation and characterization of rice straw degrading Streptomyces griseorubens C-5. Biodegradation 21(1):107–116CrossRefGoogle Scholar
  54. Yamamoto N, Asano R, Yoshii H, Otawa K, Nakai Y (2011) Archaeal community dynamics and detection of ammonia-oxidizing archaea during composting of cattle manure using culture-independent DNA analysis. Appl Microbiol Biotechnol 90(4):1501–1510CrossRefGoogle Scholar
  55. Yang F, Li G, Shi H, Wang Y (2015) Effects of phosphogypsum and superphosphate on compost maturity and gaseous emissions during kitchen waste composting. Waste Manag 36:70–76CrossRefGoogle Scholar
  56. Ye Y (2011) Identification and quantification of abundant species from pyrosequences of 16S rRNA by consensus alignment. IEEE International Conference on Bioinformatics and Biomedicine. pp. 153–157Google Scholar
  57. Yi J, Wu HY, Wu J, Deng CY, Zheng R, Chao Z (2012a) Molecular phylogenetic diversity of Bacillus community and its temporal–spatial distribution during the swine manure of composting. Appl Microbiol Biotechnol 93(1):411–421CrossRefGoogle Scholar
  58. Yi J, Zheng R, Li F, Chao Z, Deng CY, Wu J (2012b) Temporal and spatial distribution of Bacillus and Clostridium histolyticum in swine manure composting by fluorescent in situ hybridization (FISH). Appl Microbiol Biotechnol 93(6):2625–2632CrossRefGoogle Scholar
  59. Zhang F, Li Y, Ming Y, Feng C, Wei L, Yan W (2011) Change of three-dimensional excitation emission matrix fluorescence spectroscopic characterization of manure dissolved organic matter after composting and influence on its complexation with Cu. Transac Chin Soc Agric Eng 27(1):314–319Google Scholar
  60. Zhang X, Xu X, Wang J, Liu J (2012) Effect of inoculating lignin degradation strains on enzymic activities in composting. J Agro-Environ Sci 31(4):843–847Google Scholar
  61. Zhang L, Du C, Du Y, Xu M, Chen S, Liu H (2015a) Kinetic and isotherms studies of phosphorus adsorption onto natural riparian wetland sediments: linear and non-linear methods. Environ Monit Assess 187(6):381–482CrossRefGoogle Scholar
  62. Zhang Y, Wang H, Shen L, Lei Q, Jian L, He J, Zhai L, Ren T, Liu H (2015b) Identifying critical nitrogen application rate for maize yield and nitrate leaching in a Haplic Luvisol soil using the DNDC model. Sci Total Environ 514:388–398CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculty of Environmental Science & EngineeringKunming University of Science &TechnologyKunmingChina

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