Environmental Science and Pollution Research

, Volume 26, Issue 32, pp 33466–33477 | Cite as

Bioaccumulation and health risk assessment of trace metals in fish from freshwater polyculture ponds in Chengdu, China

  • Xiaoxun Xu
  • Qinglin Huo
  • Yuanyuan Dong
  • Shirong Zhang
  • Zhanbiao Yang
  • Junren Xian
  • Yuanxiang Yang
  • Zhang ChengEmail author
Research Article


The freshwater polyculture pond culturing occupied an important position in the aquaculture industry. Accumulation of trace metals was investigated in water, sediments, and fish (Carassius auratus, Cyprinus carpio, Ctenopharyngodon idellus) from typical polyculture ponds in Chengdu, China. The results showed most of the pond water in Chengdu were safe for fish cultivation. The Cd and Cr concentrations in sediment samples from sites S3, S4, and S9 which were near the industrial park and road with a high traffic volume were higher than those of the other sites. Cu, Cr, Fe, Zn, Mn, Ni, and Pb in sediments were unpolluted, while Cd was unpolluted to moderately polluted due to anthropogenic activities. Cu, Cd, and Pb in fish pond sediment of Chengdu had higher potential mobility under normal environmental circumstances. The trace metal concentrations in liver of three fish species were all higher than those in muscle tissues. The order of bioaccumulation factor (BAF) values for trace metals was Cr > Cu > Pb > Zn > Cd > Ni > 20. The concentrations of Cu, Cd, Pb, Zn, and Cr in the muscle of three fish species were all below the local and international maximum permissible levels. The target hazard quotient (THQ) and hazard index (HI) of trace metals in aquaculture fish ponds in Chengdu were lower than 1, which indicated that the consumption of grass, crucian, and common carp cultivated in the aquaculture ponds of Chengdu pose no health risk to the residents.


Chengdu Freshwater polyculture ponds Trace metals Bioaccessibility Health risk assessment 


Funding information

This study is financially supported by the Key Project of Sichuan Education Department (No. 17ZA0302), Sichuan Provincial Youth Science and Technology Fund (No. 2017JQ0035), and the National Natural Science Foundation of China (No. 21507095).

Supplementary material

11356_2019_6412_MOESM1_ESM.docx (809 kb)
ESM 1 (DOCX 808 kb)


  1. Bervoets L, Blust R, Verheyen R (2001) Accumulation of metals in the tissues of three spined stickelback (Gasterosteus aculeatus) from natural fresh waters. Ecotoxicol Environ Saf 48:117–127CrossRefGoogle Scholar
  2. Cai JN, Cao YZ, Tan HJ, Wang YM, Luo JQ (2011) Fractionation and ecological risk of metals in urban river sediments in Zhongshan City, Pearl River Delta. J Environ Monit 13:2450–2456CrossRefGoogle Scholar
  3. Chen YL, Zhun XP, Huang ZX, Wu RQ (2002) The freshwater aquaculture utility technology in southern China. Nanfang Daily Press, Guangzhou, pp 5–6Google Scholar
  4. Chen YE, Yuan S, Su YQ, Wang L (2010) Comparison of heavy metal accumulation capacity of some indigenous mosses in Southwest China cities: a case study in Chengdu city. Plant Soil Environ 56:60–66CrossRefGoogle Scholar
  5. Chen M, Pi L, Luo Y, Geng M, Hu W, Li Z, Su S, Gan Z, Ding S (2016) Grain size distribution and health risk assessment of metals in outdoor dust in Chengdu, Southwestern China. Arch Environ Contam Toxicol 70:534–543CrossRefGoogle Scholar
  6. Cheng Z, Man YB, Nie XP, Wong MH (2013) Trophic relationships and health risk assessments of trace metals in the aquaculture pond ecosystem of Pearl River Delta, China. Chemosphere 90:2142–2148CrossRefGoogle Scholar
  7. Cheng Z, Lam CL, Mo WY, Nie XP, Choi WM, Man YB, Wong MH (2016) Food wastes as fish feeds for polyculture of low-trophic-level fish: bioaccumulation and health risk assessments of heavy metals in the cultured fish. Environ Sci Pollut Res 23:7195–7203CrossRefGoogle Scholar
  8. Cheng Z, Chen LJ, Li HH, Lin JQ, Yang ZB, Yang YX, Xu XX, Xian JR, Shao JR, Zhu XM (2018) Characteristics and health risk assessment of heavy metals exposure via household dust from urban area in Chengdu, China. Sci Total Environ 619-620:621–629CrossRefGoogle Scholar
  9. Cheung KC, Leung HM, Wong MH (2008) Metal concentrations of common freshwater and marine fish from the Pearl River Delta, South China. Arch Environ Contam Toxicol 54:705–715CrossRefGoogle Scholar
  10. CNEMC (1990) China National Environmental Monitoring Center. The background values of elements in chinese soils. Environ Sci Press of China, Beijing (in Chinese)Google Scholar
  11. Diaz-de Alba M, Galindo-Riano MD, Casanueva-Marenco MJ, Garcia-Vargas M, Kosore CM (2011) Assessment of the metal pollution, potential toxicity and speciation of sediment from Algeciras Bay (South of Spain) using chemometric tools. J Hazard Mater 190:177–187CrossRefGoogle Scholar
  12. FAO/WHO (1995) General standards for contaminants and toxins in food and feed. FAO/WHO Codex Alimentarius Commission, Rome (Italy)Google Scholar
  13. GB11607 (1989) Water quality standard for fisheries. China National Standards Management Department, BeijingGoogle Scholar
  14. GB2762 (2005) Maximum levels of contaminants in foods. China National Standards Management Department, BeijingGoogle Scholar
  15. Huang PC, Tien CJ, Sun YM, Hsieh CY, Lee CC (2008) Occurrence of phthalates in sediment and biota: Relationship to aquatic factors and the biota-sediment accumulation factor. Chemosphere 73:539–544CrossRefGoogle Scholar
  16. Huang M, Wang W, Chan CY, Cheung KC, Man YB, Wang X, Wong MH (2014) Contamination and risk assessment (based on bioaccessibility via ingestion and inhalation) of metal(loid)s in outdoor and indoor particles from urban centers of Guangzhou, China. Sci Total Environ 479-480:117–124CrossRefGoogle Scholar
  17. Ikemoto T, Tu NPC, Okuda N, Iwata A, Omori K, Tanabe S, Tuyen BC (2008) Biomagnification of trace elements in the aquatic food web in the Mekong Delta, South Vietnam using stable carbon and nitrogen isotope analysis. Arch Environ Contam Toxicol 54:504–515CrossRefGoogle Scholar
  18. JECFA (2010) Summary and conclusions of the Seventy-second meeting of the Joint FAU/WHO Expert Committee on Food Additives, Joint FAO/WHO Expert Committee on Food Additives. World Health Organization, GenevaGoogle Scholar
  19. Jia Y, Kong Q, Yang Z, Wang L (2016) Accumulation behavior and risk assessment of heavy metals and arsenic in tissues of white bream (Parabramis pekinensis) from the Xiang River, southern China. Environ Sci Pollut Res 23:25056–25064CrossRefGoogle Scholar
  20. Kalyoncu L, Kalyoncu H, Arslan GJEM, Assessment (2012) Determination of heavy metals and metals levels in five fish species from Işıklı Dam Lake and Karacaören Dam Lake (Turkey). Environ Monit Assess 184:2231–2235CrossRefGoogle Scholar
  21. Lawrence AL, Mason RP (2001) Factors controlling the bioaccumulation of mercury and methylmercury by the estuarine amphipod Leptocheirus plumulosus. Environ Pollut 111:217–231CrossRefGoogle Scholar
  22. Li QS, Wu ZF, Chu B, Zhang N, Cai SS, Fang JH (2007) Heavy metals in coastal wetland sediments of the Pearl River Estuary, China. Environ Pollut 149:158–164CrossRefGoogle Scholar
  23. Li Y, Zhang Z, Liu H, Zhou H, Fan Z, Lin M, Wu D, Xia B (2016) Characteristics, sources and health risk assessment of toxic heavy metals in PM2.5 at a megacity of southwest China. Environ Geochem Health 38:353–362CrossRefGoogle Scholar
  24. Li HH, Chen LJ, Yu L, Guo ZB, Shan CQ, Lin JQ, Gu YG, Yang ZB, Yang YX, Shao JR, Zhu XM, Cheng Z (2017) Pollution characteristics and risk assessment of human exposure to oral bioaccessibility of heavy metals via urban street dusts from different functional areas in Chengdu, China. Sci Total Environ 586:1076–1084CrossRefGoogle Scholar
  25. Liang P, Shao DD, Wu SC, Shi JB, Sun XL, Wu FY, Lo SCL, Wang WX, Wong MH (2011) The influence of mariculture on mercury distribution in sediments and fish round Hong Kong and adjacent mainland China waters. Chemosphere 82:1038–1043CrossRefGoogle Scholar
  26. Liu BL, Hu K, Jiang ZL, Yang JA, Luo XM, Liu AH (2011) Distribution and enrichment of heavy metals in a sediment core from the Pearl River Estuary. Environ Earth Sci 62:265–275CrossRefGoogle Scholar
  27. Long ER, Macdonald DD, Smith SL, Calder FD (1995) Incidence of Adverse Biological Effects within Ranges of Chemical Concentrations in Marine and Estuarine Sediments. Environmental Management 19(1):81–97CrossRefGoogle Scholar
  28. McGeer JC, Brix KV, Skeaff JM, DeForest DK, Brigham SI, Adams WJ, Green A (2003) Inverse relationship between bioconcentration factor and exposure concentration for metals: implications for hazard assessment of metals in the aquatic environment. Environ Toxicol Chem 22:1017–1037CrossRefGoogle Scholar
  29. Metian M, Charbonnier L, Oberhaensli F, Bustamante P, Jeffree R, Amiard JC, Warnau M (2009) Assessment of metal, metalloid, and radionuclide bioaccessibility from mussels to human consumers, using centrifugation and simulated digestion methods coupled with radiotracer techniques. Ecotoxicol Environ Saf 72:1499–1502CrossRefGoogle Scholar
  30. Monroy M, Maceda-Veiga A, de Sostoa A (2014) Metal concentration in water, sediment and four fish species from Lake Titicaca reveals a large-scale environmental concern. Sci Total Environ 487:233–244CrossRefGoogle Scholar
  31. Moreda-Pineiro J, Moreda-Pineiro A, Romaris-Hortas V, Moscoso-Perez C, Lopez-Mahia P, Muniategui-Lorenzo S, Bermejo-Barrera P, Prada-Rodriguez D (2011) In-vivo and in-vitro testing to assess the bioaccessibility and the bioavailability of arsenic, selenium and mercury species in food samples. TrAC Trends Anal Chem 30:324–345CrossRefGoogle Scholar
  32. Moreda-Pineiro J, Moreda-Pineiro A, Romaris-Hortas V, Dominguez-Gonzalez R, Alonso-Rodriguez E, Lopez-Mahia P, Muniategui-Lorenzo S, Prada-Rodriguez D, Bermejo-Barrera P (2012) Trace metals in marine foodstuff: bioavailability estimation and effect of major food constituents. Food Chem 134:339–345CrossRefGoogle Scholar
  33. Müller G (1981) Die Schwermetallbelastung der Sedimente des Neckars und seiner Nebenflüsse Eine Bestandsaufnahme. Chemiker-Zeitung 105:157–164Google Scholar
  34. Mwakalapa EB, Simukoko CK, Mmochi AJ, Mdegela RH, Berg V, Bjorge Muller MH, Lyche JL, Polder A (2019) Heavy metals in farmed and wild milkfish (Chanos chanos) and wild mullet (Mugil cephalus) along the coasts of Tanzania and associated health risk for humans and fish. Chemosphere 224:176–186CrossRefGoogle Scholar
  35. Oomen AG, Hack A, Minekus M, Zeijdner E, Cornelis C, Schoeters G, Verstraete W, Van de Wiele T, Wragg J, Rompelberg CJM, Sips AJAM, Van Wijnen JH (2002) Comparison of five in vitro digestion models to study the bioaccessibility of soil contaminants. Environ Sci Technol 36:3326–3334CrossRefGoogle Scholar
  36. Qiao X, Schmidt AH, Tang Y, Xu YH, Zhang CS (2014) Demonstrating urban pollution using toxic metals of road dust and roadside soil in Chengdu, southwestern China. Stoch Env Res Risk A 28:911–919CrossRefGoogle Scholar
  37. Qin D, Jiang H, Bai S, Tang S, Mou Z (2015) Determination of 28 trace elements in three farmed cyprinid fish species from Northeast China. Food Control 50:1–8CrossRefGoogle Scholar
  38. USEPA (1989) Risk assessment guidance for superfund, Vol 1. EPA/540/1-89/002. Office of Emergency and Remedial Response, USEPA, Washington, DCGoogle Scholar
  39. USEPA (1997) Determination of carbon and nitrogen in sediments and particulates of Estuarine /coastal waters using elemental analysis. Available at: Accessed 22 April 2014
  40. USEPA (2018) Integrated Risk Information System (IRIS) summary table. United State Environmental Protection Agency (USEPA), Wachington, DCGoogle Scholar
  41. Wang G, Zhang S, Xiao L, Zhong Q, Li L, Xu G, Deng O, Pu Y (2017) Heavy metals in soils from a typical industrial area in Sichuan, China: spatial distribution, source identification, and ecological risk assessment. Environ Sci Pollut Res Int 24:16618–16630CrossRefGoogle Scholar
  42. Wei Y, Zhang J, Zhang D, Tu T, Luo L (2014) Metal concentrations in various fish organs of different fish species from Poyang Lake, China. Ecotoxicol Environ Saf 104:182–188CrossRefGoogle Scholar
  43. WHO (1992): Assessment of dietary intake of chemical contaminants. WHO/HPP/FOS/92.6, UNEP/GEMS/92.F2, United. Nations Environmental program, NairobiGoogle Scholar
  44. Wong MH, Cheung KC, Yediler A (2004) The dike-pond systems in South China: past, present and future. In: Wong MH (ed) Wetlands Ecosystems in Asia: Function and Management. Elsevier, Amsterdam, pp 69–86Google Scholar
  45. Zheng N, Wang Q, Zhang X, Zheng D, Zhang Z, Zhang S (2007) Population health risk due to dietary intake of heavy metals in the industrial area of Huludao City, China. Sci Total Environ 387:96–104CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of EnvironmentSichuan Agricultural UniversityChengduChina
  2. 2.Key Laboratory of Soil Environment Protection of Sichuan ProvinceSichuan Agricultural UniversityChengduChina

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