Environmental Science and Pollution Research

, Volume 26, Issue 32, pp 33702–33714 | Cite as

Greenhouse gas emissions from intact riparian wetland soil columns continuously loaded with nitrate solution: a laboratory microcosm study

  • Patteson Chula Mwagona
  • Yunlong YaoEmail author
  • Shan Yuanqi
  • Hongxian Yu
Research Article


In this study, we aimed at determining greenhouse gas (GHG) (CO2, CH4, and N2O) fluxes exchange between the soil collected from sites dominated by different vegetation types (Calamagrostis epigeios, Phragmites australis, and Carex schnimdtii) in nitrogenous loaded riparian wetland and the atmosphere. The intact soil columns collected from the wetland were incubated in laboratory and continuously treated with \( {\mathrm{NO}}_3^{-} \)-enriched water simulating downward surface water percolating through the soil to become groundwater in a natural system. This study revealed that the soil collected from the site dominated by C. epigeios was net CO2 and N2O sources, whereas the soil from P. australis and C. schnimdtii were net sinks of CO2 and N2O, respectively. The soil from the site dominated by C. schnimdtii had the highest climate impact, as it had the highest global warming potential (GWP) compared with the other sites. Our study indicates that total organic carbon and \( {\mathrm{NO}}_3^{-} \) concentration in the soil water has great influence on GHG fluxes. Carbon dioxide (CO2) and N2O fluxes were accelerated by the availability of higher \( {\mathrm{NO}}_3^{-} \) concentration in soil water. On the other hand, higher \( {\mathrm{NO}}_3^{-} \) concentration in soil water favors CH4 oxidation, hence the low CH4 production. Temporally, CO2 fluxes were relatively higher in the first 15 days and reduced gradually likely due to a decline in organic carbon. The finding of this study implies that higher \( {\mathrm{NO}}_3^{-} \) concentration in wetland soil, caused by human activities, could increase N2O and CO2 emissions from the soil. This therefore stresses the importance of controls of \( {\mathrm{NO}}_3^{-} \) leaching in the mitigation of anthropogenic N2O and CO2 emissions.


Greenhouse gas Fluxes Riparian wetland Vegetation type Microcosm experiment Soil columns Nitrate 



We would like to acknowledge all those who contributed to accomplishment of this paper.

Funding information

This work received financial support from the Fundamental Research Funds for the Central Universities (2572017CA15) and Natural Science Foundation of Heilongjiang Province (QC201604).


  1. Audet J, Hoffmann CC, Andersen PM, Baattrup-Pedersen A, Johansen JR, Larsen SE, Kjaergaard C, Elsgaard L (2014) Nitrous oxide fluxes in undisturbed riparian wetlands located in agricultural catchments: emission, uptake and controlling factors. Soil Biol Biochem 68:291–299CrossRefGoogle Scholar
  2. Beringer J, Livesley SJ, Randle J, Hutley LB (2013) Carbon dioxide fluxes dominate the greenhouse gas exchanges of a seasonal wetland in the wet–dry tropics of northern Australia. Agric For Meteorol 182:239–247CrossRefGoogle Scholar
  3. Bhullar GS, Edwards PJ, Venterink HO (2014) Influence of different plant species on methane emissions from soil in a restored Swiss wetland. PLoS One 9:e89588CrossRefGoogle Scholar
  4. Brinson MM, Lugo AE, Brown S (1981) Primary productivity, decomposition and consumer activity in freshwater wetlands. Annu Rev Ecol Syst 12:123–161CrossRefGoogle Scholar
  5. Butterbach-Bahl K, Baggs EM, Dannenmann M, Kiese R, Zechmeister-Boltenstern S (2013) Nitrous oxide emissions from soils: how well do we understand the processes and their controls? Philos Trans R Soc B 368:20130122CrossRefGoogle Scholar
  6. Change C (2001) The scientific basis.–Cambridge, United Kingdom and New York. Cambridge University Press, Cambridge 2001.–881 pGoogle Scholar
  7. Chaudhari PR, Ahire DV, Ahire VD, Chkravarty M, Maity S (2013) Soil bulk density as related to soil texture, organic matter content and available total nutrients of Coimbatore soil. Int J Sci Res Publ 3:1–8Google Scholar
  8. Chen G, Tam N, Ye Y (2010) Summer fluxes of atmospheric greenhouse gases N2O, CH4 and CO2 from mangrove soil in South China. Sci Total Environ 408:2761–2767CrossRefGoogle Scholar
  9. Cheng X, Luo Y, Xu Q, Lin G, Zhang Q, Chen J, Li B (2010) Seasonal variation in CH 4 emission and its 13 C-isotopic signature from Spartina alterniflora and Scirpus mariqueter soils in an estuarine wetland. Plant Soil 327:85–94CrossRefGoogle Scholar
  10. Chimner RA, Cooper DJ (2003) Influence of water table levels on CO2 emissions in a Colorado subalpine fen: an in situ microcosm study. Soil Biol Biochem 35:345–351CrossRefGoogle Scholar
  11. Conrad R (1996) Soil microorganisms as controllers of atmospheric trace gases (H2, CO, CH4, OCS, N2O, and NO). Microbiol Rev 60:609–640Google Scholar
  12. Couwenberg J, Dommain R, Joosten H (2010) Greenhouse gas fluxes from tropical peatlands in south-east Asia. Glob Chang Biol 16:1715–1732CrossRefGoogle Scholar
  13. Dinsmore KJ, Skiba UM, Billett MF, Rees RM (2009) Effect of water table on greenhouse gas emissions from peatland mesocosms. Plant Soil 318:229–242CrossRefGoogle Scholar
  14. Ettwig KF, Van Alen T, van de Pas-Schoonen KT, Jetten MS, Strous M (2009) Enrichment and molecular detection of denitrifying methanotrophic bacteria of the NC10 phylum. Appl Environ Microbiol 75:3656–3662CrossRefGoogle Scholar
  15. Federation WE, Association APH (2005) Standard methods for the examination of water and wastewater American Public Health Association (APHA): Washington, DC, USAGoogle Scholar
  16. Giles ME, Morley NJ, Baggs EM, Daniell TJ (2012) Soil nitrate reducing processes–drivers, mechanisms for spatial variation, and significance for nitrous oxide production. Front Microbiol 3:407CrossRefGoogle Scholar
  17. Groffman PM, Gold AJ, Addy K (2000) Nitrous oxide production in riparian zones and its importance to national emission inventories. Chemosphere-Global Change Sci 2:291–299CrossRefGoogle Scholar
  18. Hefting MM, Bobbink R, de Caluwe H (2003) Nitrous oxide emission and denitrification in chronically nitrate-loaded riparian buffer zones. J Environ Qual 32:1194–1203CrossRefGoogle Scholar
  19. Hendzel L, Matthews C, Venkiteswaran J, St. Louis V, Burton D, Joyce E, Bodaly R (2005) Nitrous oxide fluxes in three experimental boreal forest reservoirs. Environ Sci Technol 39:4353–4360. CrossRefGoogle Scholar
  20. Huang W, Chen Q, Ren K, Chen K (2015) Vertical distribution and retention mechanism of nitrogen and phosphorus in soils with different macrophytes of a natural river mouth wetland. Environ Monit Assess 187:97CrossRefGoogle Scholar
  21. Huttunen JT, Alm J, Liikanen A, Juutinen S, Larmola T, Hammar T, Silvola J, Martikainen PJ (2003) Fluxes of methane, carbon dioxide and nitrous oxide in boreal lakes and potential anthropogenic effects on the aquatic greenhouse gas emissions. Chemosphere 52:609–621CrossRefGoogle Scholar
  22. IPCC (2013) Climate change 2013: the physical science basis. In: Stocker TF, Qin D, Plattner G-K, Tignor M, Allen SK, Boschung J, Nauels A, Xia Y, Bex V, Midgley PM (eds) Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, Cambridge, United Kingdom and New York, NY, USA 1535 ppGoogle Scholar
  23. Jacinthe P, Bills J, Tedesco L, Barr R (2012) Nitrous oxide emission from riparian buffers in relation to vegetation and flood frequency. J Environ Qual 41:95–105CrossRefGoogle Scholar
  24. Jørgensen CJ, Struwe S, Elberling B (2012) Temporal trends in N2O flux dynamics in a Danish wetland–effects of plant-mediated gas transport of N2O and O2 following changes in water level and soil mineral-N availability. Glob Chang Biol 18:210–222CrossRefGoogle Scholar
  25. Jugsujinda A, DeLaune R, Lindau C (1995) Influence of nitrate on methane production and oxidation in flooded soil. Commun Soil Sci Plant Anal 26:2449–2459CrossRefGoogle Scholar
  26. Juszczak R, Augustin J (2013) Exchange of the greenhouse gases methane and nitrous oxide between the atmosphere and a temperate peatland in central. Eur Wetl 33:895–907CrossRefGoogle Scholar
  27. Kayranli B, Scholz M, Mustafa A, Hedmark Å (2010) Carbon storage and fluxes within freshwater wetlands: a critical review. Wetlands 30:111–124CrossRefGoogle Scholar
  28. Klüber HD, Conrad R (1998) Effects of nitrate, nitrite, NO and N2O on methanogenesis and other redox processes in anoxic rice field soil. FEMS Microbiol Ecol 25:301–318CrossRefGoogle Scholar
  29. Kögel-Knabner I, Amelung W, Cao Z, Fiedler S, Frenzel P, Jahn R, Kalbitz K, Kölbl A, Schloter M (2010) Biogeochemistry of paddy soils. Geoderma 157:1–14CrossRefGoogle Scholar
  30. Lashof DA, Ahuja DR (1990) Relative contributions of greenhouse gas emissions to global warming. Nature 344:529–531CrossRefGoogle Scholar
  31. Laverman AM, Garnier JA, Mounier EM, Roose-Amsaleg CL (2010) Nitrous oxide production kinetics during nitrate reduction in river sediments. Water Res 44:1753–1764CrossRefGoogle Scholar
  32. Li L, Xing M, Lv J, Wang X, Chen X (2017) Response of rhizosphere soil microbial to Deyeuxia angustifolia encroaching in two different vegetation communities in alpine tundra. Sci Rep 7:43150CrossRefGoogle Scholar
  33. Lind LP, Audet J, Tonderski K, Hoffmann CC (2013) Nitrate removal capacity and nitrous oxide production in soil profiles of nitrogen loaded riparian wetlands inferred by laboratory microcosms. Soil Biol Biochem 60:156–164CrossRefGoogle Scholar
  34. Liu Y, Liu G, Xiong Z, Liu W (2017) Response of greenhouse gas emissions from three types of wetland soils to simulated temperature change on the Qinghai-Tibetan Plateau. Atmos Environ 171:17–24CrossRefGoogle Scholar
  35. Lorrain M-J, Tartakovsky B, Peisajovich-Gilkstein A, Guiot S (2004) Comparison of different carbon sources for ground water denitrification. Environ Technol 25:1041–1049CrossRefGoogle Scholar
  36. Mata-Alvarez J, Mace S, Llabres P (2000) Anaerobic digestion of organic solid wastes. An overview of research achievements and perspectives. Bioresour Technol 74:3–16CrossRefGoogle Scholar
  37. Melillo JM, Aber JD, Muratore JF (1982) Nitrogen and lignin control of hardwood leaf litter decomposition dynamics. Ecology 63:621–626CrossRefGoogle Scholar
  38. Micks P, Aber JD, Boone RD, Davidson EA (2004) Short-term soil respiration and nitrogen immobilization response to nitrogen applications in control and nitrogen-enriched temperate forests. For Ecol Manag 196:57–70CrossRefGoogle Scholar
  39. Muñoz-Hincapié M, Morell JM, Corredor JE (2002) Increase of nitrous oxide flux to the atmosphere upon nitrogen addition to red mangroves sediments. Mar Pollut Bull 44:992–996CrossRefGoogle Scholar
  40. Mwagona PC, Yao Y, Yuanqi S, Yu H (2019) Laboratory study on nitrate removal and nitrous oxide emission in intact soil columns collected from nitrogenous loaded riparian wetland, Northeast China. PLoS One 14:e0214456CrossRefGoogle Scholar
  41. Myhre G et al (2013) Climate change 2013: the physical science basis. In: Tignor M, Allen SK, Boschung J, Nauels A, Xia Y, Bex V, Midgley PM (eds) Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change K. Cambridge University Press, Cambridge, United Kingdom and New York, NY, USAGoogle Scholar
  42. Nag SK, Liu R, Lal R (2017) Emission of greenhouse gases and soil carbon sequestration in a riparian marsh wetland in central Ohio. Environ Monit Assess 189:580CrossRefGoogle Scholar
  43. Olsson L, Ye S, Yu X, Wei M, Krauss KW, Brix H (2015) Factors influencing CO 2 and CH 4 emissions from coastal wetlands in the Liaohe Delta, Northeast China. Biogeosciences 12:4965–4977CrossRefGoogle Scholar
  44. Rivett MO, Buss SR, Morgan P, Smith JW, Bemment CD (2008) Nitrate attenuation in groundwater: a review of biogeochemical controlling processes. Water Res 42:4215–4232CrossRefGoogle Scholar
  45. Schlesinger WH (2013) An estimate of the global sink for nitrous oxide in soils. Glob Chang Biol 19:2929–2931CrossRefGoogle Scholar
  46. Song C, Wang Y, Wang Y, Zhao Z (2006) Emission of CO2, CH4 and N2O from freshwater marsh during freeze–thaw period in Northeast of China. Atmos Environ 40:6879–6885CrossRefGoogle Scholar
  47. Song C, Zhang J, Wang Y, Wang Y, Zhao Z (2008) Emission of CO2, CH4 and N2O from freshwater marsh in northeast of China. J Environ Manag 88:428–436. CrossRefGoogle Scholar
  48. Song C, Xu X, Tian H, Wang Y (2009) Ecosystem–atmosphere exchange of CH4 and N2O and ecosystem respiration in wetlands in the Sanjiang Plain, Northeastern China. Glob Chang Biol 15:692–705CrossRefGoogle Scholar
  49. Song C, Liu D, Yang G, Song Y, Mao R (2011) Effect of nitrogen addition on decomposition of Calamagrostis angustifolia litters from freshwater marshes of Northeast China. Ecol Eng 37:1578–1582CrossRefGoogle Scholar
  50. Song D, Su M, Yang J, Chen B (2012) Greenhouse gas emission accounting and management of low-carbon community. Sci World J 2012:1–6Google Scholar
  51. Song Y-Y, Song C-C, Ren J-S, Zhang X-H, Jiang L (2018) Nitrogen input increases Deyeuxia angustifolia litter decomposition and enzyme activities in a marshland ecosystem in Sanjiang Plain, Northeast China. Wetlands:1–9Google Scholar
  52. Ström L, Christensen TR (2007) Below ground carbon turnover and greenhouse gas exchanges in a sub-arctic wetland. Soil Biol Biochem 39:1689–1698CrossRefGoogle Scholar
  53. Sun Z, Wang L, Tian H, Jiang H, Mou X, Sun W (2013) Fluxes of nitrous oxide and methane in different coastal Suaeda salsa marshes of the Yellow River estuary, China. Chemosphere 90:856–865CrossRefGoogle Scholar
  54. Syakila A, Kroeze C, Slomp CP (2010) Neglecting sinks for N2O at the earth’s surface: does it matter? J Integr Environ Sci 7:79–87CrossRefGoogle Scholar
  55. Team RC (2014) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria 2013. ISBN 3-900051-07-0,Google Scholar
  56. Tian H, Xu X, Lu C, Liu M, Ren W, Chen G, Melillo J, Liu J (2011) Net exchanges of CO2, CH4, and N2O between China’s terrestrial ecosystems and the atmosphere and their contributions to global climate warming. J Geophys Res Biogeosci 116Google Scholar
  57. Uddin MN, Robinson RW (2017) Responses of plant species diversity and soil physical-chemical-microbial properties to Phragmites australis invasion along a density gradient. Sci Rep 7:11007CrossRefGoogle Scholar
  58. Wang Z, Mao D, Li L, Jia M, Dong Z, Miao Z, Ren C, Song C (2015) Quantifying changes in multiple ecosystem services during 1992–2012 in the Sanjiang Plain of China. Sci Total Environ 514:119–130CrossRefGoogle Scholar
  59. Wang Q, Kwak J-H, Choi W-J, Chang SX (2018) Decomposition of trembling aspen leaf litter under long-term nitrogen and sulfur deposition: effects of litter chemistry and forest floor microbial properties. For Ecol Manag 412:53–61CrossRefGoogle Scholar
  60. Weier K, Doran J, Power J, Walters D (1993) Denitrification and the dinitrogen/nitrous oxide ratio as affected by soil water, available carbon, and nitrate. Soil Sci Soc Am J 57:66–72CrossRefGoogle Scholar
  61. Wu D, Dong W, Oenema O, Wang Y, Trebs I, Hu C (2013) N2O consumption by low-nitrogen soil and its regulation by water and oxygen. Soil Biol Biochem 60:165–172CrossRefGoogle Scholar
  62. Xu X, Zou X, Cao L, Zhamangulova N, Zhao Y, Tang D, Liu D (2014) Seasonal and spatial dynamics of greenhouse gas emissions under various vegetation covers in a coastal saline wetland in southeast China. Ecol Eng 73:469–477CrossRefGoogle Scholar
  63. Yan X, Du L, Shi S, Xing G (2000) Nitrous oxide emission from wetland rice soil as affected by the application of controlled-availability fertilizers and mid-season aeration. Biol Fertil Soils 32:60–66CrossRefGoogle Scholar
  64. Yanai J et al (2003) Spatial variability of nitrous oxide emissions and their soil-related determining factors in an agricultural field. J Environ Qual 32:1965–1977CrossRefGoogle Scholar
  65. Yang Z, Zhao Y, Xia X (2012) Nitrous oxide emissions from Phragmites australis-dominated zones in a shallow lake. Environ Pollut 166:116–124CrossRefGoogle Scholar
  66. Yang J, Liu J, Hu X, Li X, Wang Y, Li H (2013) Effect of water table level on CO2, CH4 and N2O emissions in a freshwater marsh of Northeast China. Soil Biol Biochem 61:52–60CrossRefGoogle Scholar
  67. Zhang W, Tian Z, Zhang N, Li X (1996) Nitrate pollution of groundwater in northern China Agriculture. Ecosyst Environ 59:223–231CrossRefGoogle Scholar
  68. Zhang J-b, Song C-c, Yang W-y (2005) Cold season CH4, CO2 and N2O fluxes from freshwater marshes in northeast China. Chemosphere 59:1703–1705CrossRefGoogle Scholar
  69. Zhang G-L, Zhang J, Liu S-M, Ren J-L, Zhao Y-C (2010) Nitrous oxide in the Changjiang (Yangtze River) Estuary and its adjacent marine area: Riverine input, sediment release and atmospheric fluxes. Biogeosciences 7:3505–3516CrossRefGoogle Scholar
  70. Zhang L, Song L, Zhang L, Shao H, Chen X, Yan K (2013) Seasonal dynamics in nitrous oxide emissions under different types of vegetation in saline-alkaline soils of the Yellow River Delta, China and implications for eco-restoring coastal wetland. Ecol Eng 61:82–89. CrossRefGoogle Scholar
  71. Zhang X, Mao R, Song C, Song Y, Finnegan PM (2017) Nitrogen addition in a freshwater marsh alters the quality of senesced leaves, promoting decay rates and changing nutrient dynamics during the standing-dead phase. Plant Soil 417:511–521CrossRefGoogle Scholar
  72. Zhaofu L, Xianguo L, Qing Y (2005) Soil-surface CO 2 fluxes in a Deyeuxia angustifolia wetland in Sanjiang Plain, China. Wetl Ecol Manag 13:35–41CrossRefGoogle Scholar

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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Wildlife and Protected AreaNortheast Forestry UniversityHarbinPeople’s Republic of China

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