Environmental Science and Pollution Research

, Volume 26, Issue 30, pp 30930–30940 | Cite as

Effects of mercury on the germination and growth of Quercus ilex L. seedlings

  • Javier Rodríguez-AlonsoEmail author
  • María José Sierra
  • Miguel Ángel Lominchar
  • Rocío Millán
Research Article


While it is well-known that the toxicity of mercury for plants is related to its bioavailability in the environment in which the plant lives, few studies have addressed Hg effects under controlled conditions of life-limiting available Hg concentrations. This study examines the effects of Hg on the holm oak (Quercus ilex L.) exposed to medium-high available Hg concentrations. Holm oak seeds were sown in a perlite substrate and grown in the presence of a nutrient solution containing 0, 5, 25, or 50 μM Hg. The variables determined as outcome measures were impacts on germination, growth, and nutrient accumulation along with Hg concentration in leaves, stems, and roots at different growth stages. Our findings suggest no overall detrimental effects of the metal on germination, nutrient accumulation, and plant growth, although root morphology was clearly modified. Mercury accumulation in the plant varied according to time, organ, Hg treatment dose, and plant growth stage. When comparing Hg build-up in the different organs, highest concentrations of the metal were detected in the roots, followed by the leaves and stems. The Hg accumulation pattern was positively correlated with time and Hg dose, whereas negative correlation was observed with growth stage. The impacts of all these factors on Hg accumulation were not additive pointing to interesting interaction effects that should be explored in future work.


Holm oak Growth stage Mercury accumulation Nutrient accumulation Interaction effects 



We are grateful to all the members of the CIEMAT Research Unit “Conservación y Recuperación de Suelos” for the essential help in the second sampling effort, when hundreds of holm oak seedlings had to be washed, weighed, and measured in just 4 days.

Supplementary material

11356_2019_6186_MOESM1_ESM.pptx (98 kb)
ESM 1 (PPTX 98 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Javier Rodríguez-Alonso
    • 1
    Email author
  • María José Sierra
    • 1
  • Miguel Ángel Lominchar
    • 1
  • Rocío Millán
    • 1
  1. 1.CIEMAT—Environmental Department (DMA)MadridSpain

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