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Environmental Science and Pollution Research

, Volume 26, Issue 28, pp 28951–28961 | Cite as

Zinc-lysine prevents chromium-induced morphological, photosynthetic, and oxidative alterations in spinach irrigated with tannery wastewater

  • Ihsan Elahi Zaheer
  • Shafaqat AliEmail author
  • Muhammad Rizwan
  • Firdaus-e- Bareen
  • Zohaib Abbas
  • Syed Asad Hussain Bukhari
  • Leonard Wijaya
  • Mohammed Nasser Alyemeni
  • Parvaiz AhmadEmail author
Research Article
  • 99 Downloads

Abstract

Anthropogenic activities have resulted in severe environmental degradation. Untreated wastewater from tanneries is hazardous to all kinds of life on earth. Effluent from tanning industries, containing large amount of Cr, is used to irrigate the crops in Pakistan. The current experiment was carried out to study the effects of tannery wastewater on spinach and the role of lysine-Zn in mitigating the severity of stress. The plants were grown in soil and the following treatments were used: irrigation with 0%, 33%, 66%, and 100% wastewater (ww) along with two doses (0 mM, 10 mM) of Zn-lysine. Foliar application of zinc-lysine enhanced the plant growth, biomass, Zn contents, photosynthesis, and enzyme activities in different tissues of plant. Zinc-lysine (10 mM) considerably decreased the Cr content in roots and shoots, along with ameliorating the oxidative stress by enhancing the activities of antioxidant enzymes in plants. Addition of Zn-lys (10 mM) improved the plant height by 19%, root length by 57%, leaf dry weight by 19%, and root dry weight by33% under 100% Cr treatment. Zn-lys significantly reduces the oxidative stress and concentration of Cr as compared with the Cr treatments alone. Application of Zn-lys (10 mM) reduced the Cr contents in roots by 27 and 22 under 33 and 66% Cr treatment, respectively. Taken together, Zn-lys chelates efficiently ameliorated the toxic effects of chromium. Zn-lysine has the extravagant potential of mitigating the heavy metal toxicity without harming the normal growth and development of the plants.

Keywords

Chromium stress Zn-lysine Growth attributes Oxidative stress Antioxidant enzymes Tannery wastewater irrigation 

Notes

Acknowledgements

The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for funding this Research group NO.(RGP-199).

Funding information

This work was financially supported by the Government College University, Faisalabad, and Higher Education Commission of Pakistan under HEC Project No. 20-3653/NRPU/R&D/HEC/14/437 and NRPU Project No. 5634/Punjab/NRPU/ R&D/HEC/2016

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Aebi H (1984) Catalase in vitro. In: Methods in enzymology, vol 105. Academic Press, pp 121–126Google Scholar
  2. Afshan S, Ali S, Bharwana SA, Rizwan M, Farid M, Abbas F, Ibrahim M, Mehmood MA, Abbasi GH (2015) Citric acid enhances the phytoextraction of chromium, plant growth, and photosynthesis by alleviating the oxidative damages in Brassica napus L. Environ Sci Pollut Res 22:11679–11689Google Scholar
  3. Ahmad R, Ali S, Hannan F, Rizwan M, Iqbal M, Hassan Z, Akram NA, Maqbool S, Abbas F (2017) Promotive role of 5-aminolevulinic acid on chromium-induced morphological, photosynthetic, and oxidative changes in cauliflower (Brassica oleracea botrytis L.). Environ Sci Pollut Res 24:8814–8824Google Scholar
  4. Ali S, Farooq MA, Jahangir MM, Abbas F, Bharwana SA, Zhang GP (2013) Effect of chromium and nitrogen form on photosynthesis and anti-oxidative system in barley. Biol Plant 57(4):758–763Google Scholar
  5. Ali S, Bharwana SA, Rizwan M, Farid M, Kanwal S, Ali Q, Khan MD (2015) Fulvic acid mediates chromium (Cr) tolerance in wheat (Triticum aestivum L.) through lowering of Cr uptake and improved antioxidant defense system. Environ Sci Pollut Res 22(14):10601–10609Google Scholar
  6. Ali S, Rizwan M, Waqas A, Hussain MB, Hussain A, Liu S, Abd_Allah EF (2018) Fulvic acid prevents chromium-induced morphological, photosynthetic, and oxidative alterations in wheat irrigated with tannery waste water. J Plant Growth Regul 37(4):1357–1367Google Scholar
  7. Amacher MC (1996) Nickel, cadmium and lead. 739–768. In: Sparks DL (ed) Methods of soil analysis. Part 3. Chemical methods, 3rd edn. SSSA/ASA, MadisonGoogle Scholar
  8. Anjum SA, Ashraf U, Khan I, Saleem MF, Wang LC (2016) Chromium toxicity induced alterations in growth, photosynthesis, gas exchange attributes and yield formation in maize. Pak J Agric Sci 53:751–757Google Scholar
  9. APHA (2005) Standard Methods for the Examination of Water and Wastewater. 21st Edition, American Public Health Association/American Water Works Association/Water Environment Federation, Washington DCGoogle Scholar
  10. Ashraf S, Afzal M, Rehman K, Naveed M, Zahi ZA (2018) Plant-endophyte synergism in constructed wetlands enhances the remediation of tannery effluent. Water Sci Technol 77(5):1262–1270Google Scholar
  11. Ayers, RS and Westcot, DW (1985) Water Quality for Agriculture. FAO Irrigation and Drainage Paper 29 Rev. 1., FAO, RomeGoogle Scholar
  12. Bashir A, Rizwan M, Ali S, ur Rehman MZ, Ishaque W, Riaz MA, Maqbool A (2018) Effect of foliar-applied iron complexed with lysine on growth and cadmium (Cd) uptake in rice under Cd stress. Environ Sci Pollut Res 25(21):20691–20699Google Scholar
  13. Bouyoucos GJ (1962) Hydrometer method improved for making particle size analyses of soils. Agron J 54(5):464–465Google Scholar
  14. Bukhari SAH, Shang S, Zhang M, Zheng W, Zhang G, Wang TZ, Shamsi IH, Wu FB (2015) Genome-wide identification of chromium stress-responsive microRNAs and their target genes in tobacco (Nicotiana tabacum) roots. Environ Toxicol Chem 34(11):2573–2582Google Scholar
  15. Bukhari SAH, Wang R, Wang W, Ahmed IM, Zheng W, Cao F (2016) Genotype-dependent effect of exogenous 24-epibrassinolide on chromium-induced changes in ultrastructure and physicochemical traits in tobacco seedlings. Environ Sci Pollut Res 23(18):18229–18238Google Scholar
  16. Bukhari SAH, Zheng W, Xie L, Zhang G, Shang S, Wu F (2016a) Cr-induced changes in leaf protein profile, ultrastructure and photosynthetic traits in the two contrasting tobacco genotypes. Plant Growth Regul 79(2):147–156Google Scholar
  17. Dionisio-Sese ML, Tobita S (1998) Antioxidant responses of rice seedlings to salinity stress. Plant Sci 135:1–9Google Scholar
  18. Dotaniya ML, Rajendiran S, Coumar MV, Meena VD, Saha JK, Kundu S, Kumar A, Patra AK (2018) Interactive effect of cadmium and zinc on chromium uptake in spinach grown in Vertisol of Central India. Int J Environ Sci Technol 15:441–448Google Scholar
  19. Farid M, Ali S, Akram NA, Rizwan M, Abbas F, Bukhari SAH, Saeed R (2017) Phyto-management of Cr-contaminated soils by sunflower hybrids: physiological and biochemical response and metal extractability under Cr stress. Environ Sci Pollut Res 24(20):16845–16859Google Scholar
  20. Farid M, Ali S, Saeed R, Rizwan M, Bukhari SAH, Abbasi GH, Hussain A, Ali B, Ibni Zamir MS, Ahmad I (2019) Combined application of citric acid and 5-aminolevulinic acid improved biomass, photosynthesis and gas exchange attributes of sunflower (Helianthus annuus L.) grown on chromium contaminated soil. Int J Phytoremediation.  https://doi.org/10.1080/15226514.2018.1556595 Google Scholar
  21. Ghasemi S, Khoshgoftarmanesh AH, Hadadzadeh H, Jafari M (2012) Synthesis of iron amino acid chelates and evaluation of their efficacy as iron source and growth stimulator for tomato in nutrient solution culture. J Plant Growth Regul 31:498–508Google Scholar
  22. Ghasemi S, Khoshgoftarmanesh AH, Hadadzadeh H, Afyuni M (2013a) Synthesis, characterization, and theoretical and experimental investigations of zinc (II)–amino acid complexes as ecofriendly plant growth promoters and highly bioavailable sources of zinc. J Plant Growth Regul 32:315–323Google Scholar
  23. Ghasemi S, Khoshgoftarmanesh AH, Afyuni M, Hadadzadeh H (2013b) The effectiveness of foliar applications of synthesized zinc-amino acid chelates in comparison with zinc sulfate to increase yield and grain nutritional quality of wheat. Eur J Agron 45:68–74Google Scholar
  24. Ghasemi S, Khoshgoftarmanesh AH, Afyuni M, Hadadzadeh H (2014) Iron (II)–amino acid chelates alleviate salt-stress induced oxidative damages on tomato grown in nutrient solution culture. Sci Hortic 165:91–98Google Scholar
  25. Gill RA, Zang L, Ali B, Farooq MA, Cui P, Yang S, Zhou W (2015) Chromium-induced physio-chemical and ultrastructural changes in four cultivars of Brassica napus L. Chemosphere 120:154–164Google Scholar
  26. Gill RA, Zhang N, Ali B, Farooq MA, Xu J, Gill MB, Mao B, Zhou W (2016) Role of exogenous salicylic acid in regulating physiomorphic and molecular changes under chromium toxicity in black and yellow-seeded Brassica napus L. Environ Sci Pollut Res 23(20):20483–20496Google Scholar
  27. Gomes MAD, Hauser-Davis RA, Suzuki MS, Vitória AP (2017) Plant chromium uptake and transport, physiological effects and recent advances in molecular investigations. Ecotoxicol Environ Saf 140:55–64Google Scholar
  28. Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198Google Scholar
  29. Hussain A, Ali S, Rizwan M, ur Rehman MZ, Hameed A, Hafeez F, Wijaya L (2018) Role of zinc–lysine on growth and chromium uptake in rice plants under cr stress. J Plant Growth Regul 37(4):1413–1422Google Scholar
  30. Ilyas A, Ashraf MY, Hussain M, Ashraf M, Ahmed R, Kamal A (2015) Effect of micronutrients (Zn, Cu And B) on photosynthetic and fruit yield attributes of Citrus reticulata Blanco Var. Kinnow. Pak J Bot 47(4):1241–1247Google Scholar
  31. Jabeen N, Abbas Z, Iqbal M, Rizwan M, Jabbar A, Farid M, Ali S, Ibrahim M, Abbas F (2016) Glycinebetaine mediates chromium tolerance in mung bean through lowering of Cr uptake and improved antioxidant system. Arch Agron Soil Sci 62:648–662Google Scholar
  32. Khalid S, Shahid M, Dumat C, Niazi NK, Bibi I, Gul Bakhat HFS, Javeed HMR (2017) Influence of groundwater and wastewater irrigation on lead accumulation in soil and vegetables: implications for health risk assessment and phytoremediation. Int J Phytoremediation 19(11):1037–1046Google Scholar
  33. Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. In: Colowick SP, Kaplan NO (ed.) Methods in cenzymology. Academic Press, San Diego-New YorkBerkeley-Boston-London-Sydney-Tokyo-Toronto, 148:350–382Google Scholar
  34. Maqbool A, Ali S, Rizwan M, Ishaque W, Rasool N, ur Rehman MZ, Wu L (2018) Management of tannery wastewater for improving growth attributes and reducing chromium uptake in spinach through citric acid application. Environ Sci Pollut Res 25(11):10848–10856Google Scholar
  35. Mohammadi P, Khoshgoftarmanesh AH (2014) The effectiveness of synthetic zinc (Zn)-amino 406 chelates in supplying Zn and alleviating salt-induced damages on hydroponically grown lettuce. Sci Hortic 172:117–123Google Scholar
  36. Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar
  37. Nasholm T, Kielland K, Ganeteg U (2009) Uptake of organic nitrogen by plants. New Phytol 182:31–48Google Scholar
  38. Noman A, Ali Q, Maqsood J, Iqbal N, Javed MT, Rasool N, Naseem J (2018) Deciphering physio-biochemical, yield, and nutritional quality attributes of water-stressed radish (Raphanus sativus L.) plants grown from Zn-Lys primed seeds. Chemosphere 195:175–189Google Scholar
  39. Oliveira H (2012) Chromium as an environmental pollutant: insights on induced plant toxicity. Aust J Bot 2012:1–8.  https://doi.org/10.1155/2012/375843 CrossRefGoogle Scholar
  40. Page AL, Miller RH, Keeny DR (1982) Methods of soil analysis (Part 2). Chemical and microbiological properties. Agron 9. SSSA, MadisonGoogle Scholar
  41. Rafie MR, Khoshgoftarmanesh AH, Shariatmadari H, Darabi A, Dalir N (2017) Influence of foliar-applied zinc in the form of mineral and complexed with amino acids on yield and nutritional quality of onion under field conditions. Sci Hortic 216:160–168Google Scholar
  42. Rehman MZ, Rizwan M, Ghafoor A, Naeem A, Ali S, Sabir M, Qayyum MF (2015) Effect of inorganic amendments for in situ stabilization of cadmium in contaminated soils and its phyto-availability to wheat and rice under rotation. Environ Sci Pollut Res 22:16897–16906Google Scholar
  43. Rivelli AR, Puschenreiter M, De Maria S (2014) Assessment of cadmium uptake and nutrient content in sunflower plants grown under Cd stress. Plant Soil Environ 60:80–86Google Scholar
  44. Rizwan M, Ali S, Hussain A, Ali Q, Shakoor MB, Zia-ur-Rehman M, Asma M (2017) Effect of zinc-lysine on growth, yield and cadmium uptake in wheat (Triticum aestivum L.) and health risk assessment. Chemosphere 187:35–42Google Scholar
  45. Rizwan M, Ali S, Abbas F, Adrees M, Zia-ur-Rehman M, Farid M, Gill RA, Ali B (2017a) Role of organic and inorganic amendments in alleviating heavy metal stress in oil seed crops. In: Ahmad P (ed) Environ Sci Pollut Res Oil Seed Crops: Yield and Adaptations under Environmental Stress, 1st edn, vol. 12. John Wiley & Sons, Ltd., 224–235Google Scholar
  46. Sadak MS, Abdelhamid MT (2015) Influence of amino acids mixture application on some biochemical aspects, antioxidant enzymes and endogenous polyamines of Vicia faba plant grown under seawater salinity stress. Gesunde Pflnzen 67:119–129Google Scholar
  47. Sehrish AK, Aziz R, Hussain MM, Rafiq MT, Rizwan M, Muhammad N, Rafiq MK, Sehar A, Din J, Al-Wabel MI, Ali S (2019) Effect of poultry litter biochar on chromium (Cr) bioavailability and accumulation in spinach (Spinacia oleracea) grown in Cr-polluted soil. Arab J Geosci 12:57Google Scholar
  48. Shahid M, Shamshad S, Rafique M, Khalid S, Bibi I, Niazi KN, Dumat C, Rashid IM (2017) Chromium speciation, bioavailability, uptake, toxicity and detoxification in soil -plant system -a review. Chemosphere 178:513–533Google Scholar
  49. Sharma SS, Dietz KJ (2006) The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress. J Exp Bot 57:711–726Google Scholar
  50. Sharma P, Kumar A, Bhardwaj R (2016) Plant steroidal hormone epibrassinolide regulate: heavy metal stress tolerance in Oryza sativa L. by modulating antioxidant defense expression. Environ Exp Bot 122:1–9Google Scholar
  51. Singh A, Malaviya P (2019) Chromium phytoaccumulation and its impact on growth and photosynthetic pigments of Spirodela polyrrhiza (L.) Schleid. on exposure to tannery effluent. Environ Sustain 2(2):157–166Google Scholar
  52. Singh HP, Mahajan P, Kaur S, Batish DR, Kohli RK (2013) Chromium toxicity and tolerance in plants. Environ Chem Lett 11:229–254Google Scholar
  53. Soltanpour PN (1985) Use of AB-DTPA soil test to evaluate elemental availability and toxicity. Commun Soil Sci Plant Anal 16(3):323–338Google Scholar
  54. Souri MK (2016) Amino chelate fertilizers: the new approach to the old problem; a review. Open Agric 1:118–123Google Scholar
  55. Tauqeer HM, Ali S, Rizwan M, Ali Q, Saeed R, Iftikhar U, Ahmad R, Farid M, Abbasi GH (2016) Phytoremediation of heavy metals by Alternanthera bettzickiana: growth and physiological response. Ecotoxicol Environ Saf 126:138–146Google Scholar
  56. Teixeira WF, Fagan EB, Soares LH, Umburanas RC, Reichardt K, Neto DD (2017) Foliar and seed application of amino acids affects the antioxidant metabolism of the soybean crop. Front Plant Sci 8:1–14Google Scholar
  57. US Salinity Laboratory Staff (1954) Diagnosis and improvement of saline and alkali soils. Agriculture handbook 60. United States Salinity Laboratory, USDA, Washington DC, p 160Google Scholar
  58. Walkley A, Black IA (1934) An examination of the Degtjareff method for determining in soil organic matter, and a proposed modification of the chromic soil titration method. Soil Sci 37:29–38Google Scholar
  59. Zaman QU, Aslam Z, Yaseen M, Ihsan MZ, Khaliq A, Fahad S, Bashir S, Ramzani PM, Naeem M (2017) Zinc biofortifiation in rice: leveraging agriculture to moderate hidden hunger in developing countries. Arch Agron Soil Sci 64(2): 147–161Google Scholar
  60. Zhang XZ (1992) The measurement and mechanism of lipid peroxidation and SOD, POD and CAT activities in biological system, Research methodology of crop physiology. Agriculture Press, Beijing, pp 208–211Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Ihsan Elahi Zaheer
    • 1
  • Shafaqat Ali
    • 1
    Email author
  • Muhammad Rizwan
    • 1
  • Firdaus-e- Bareen
    • 2
  • Zohaib Abbas
    • 1
  • Syed Asad Hussain Bukhari
    • 3
  • Leonard Wijaya
    • 4
  • Mohammed Nasser Alyemeni
    • 4
  • Parvaiz Ahmad
    • 4
    • 5
    Email author
  1. 1.Department of Environmental Sciences and EngineeringGovernment College UniversityFaisalabadPakistan
  2. 2.Department of BotanyUniversity of the PunjabLahorePakistan
  3. 3.Department of AgronomyBahauddin Zakariya UniversityMultanPakistan
  4. 4.Botany and Microbiology Department, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  5. 5.Department of BotanyS.P. CollegeJammu and KashmirIndia

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