Propolis extract protects against radiation-induced intestinal mucositis through anti-apoptotic mechanisms
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Intestinal mucositis is a common side effect during radiotherapy that could be largely prevented by compounds possessing anti-inflammatory or anti-oxidant properties, including extracts of propolis containing a high proportion of flavonoids. A specially formulated aqueous extract of propolis (PWE) has been prepared in such a way to preclude the inclusion of flavonoids but contain mostly organic aromatic acids to study whether it would still protect against radiation-induced intestinal mucositis and to study the possible involvement of apoptotic pathways. Rats were exposed to a gamma radiation dose of 8 Gy from a Cesium-137 source in order to inflict intestinal mucositis. Three days before exposure, rats were given PWE orally and treatment continued for 2 more days. Twenty-four hours later, rats were sacrificed, the small intestine was excised, and sections were examined histologically. Different parameters for apoptosis, inflammation, and oxidative stress were determined in the serum and in intestinal homogenates. Radiation exposure led to histological and biochemical signs of intestinal damage. This was associated with an increase in apoptotic indicators and derangement in oxidative stress parameters. All deranged parameters were largely prevented by PWE. The findings provide evidence that the protective effect of PWE against intestinal radiation damage involves not only its anti-inflammatory and anti-oxidant effects but also its anti-apoptotic properties as well.
KeywordsPropolis Intestinal mucositis Gamma irradiation Apoptosis
The authors wish to acknowledge the contribution of Dr. Jens Nielsen, Propolis Research Centre, Propharma A/S (Blistrup, Denmark), for providing PWE and for his valuable advice. The help of Dr. Kawkab Abdel-Aziz Ahmed, Professor of Pathology, Faculty of Veterinary Medicine, Cairo University, for carrying out the histological examinations is also gratefully acknowledged.
Compliance with ethical standards
The study was conducted according to the guidelines of the European Communities Council Directive (86/609/EEC) and approved by the Ethical Committee for Animal Experimentation at the Faculty of Pharmacy, Cairo University (PT (85)).
Conflict of interest
The authors declare that they have no conflict of interest.
- Beutler E, Duron O, Kelly BM (1963) Improved method for the determination of blood glutathione. J Lab Clin Med 61:882–888Google Scholar
- Bradley PP, Christensen RD, Rothstein G (1982) Cellular and extracellular myeloperoxidase in pyogenic inflammation. Blood 60:618–622Google Scholar
- Chen SD, Chen YB, Peng Y, Xu J, Chen SS, Zhang JL, Li ZZ, Tan Z (2010) Role of PI3K/Akt signaling in the protective effect of magnesium sulfate against ischemia-perfusion injury of small intestine in rats. Chin Med J 123:1447–1452Google Scholar
- Driák D, Osterreicher J, Vávrová J, Reháková Z, Vilasová Z (2008) Morphological changes of rat jejunum after whole body gamma-irradiation and their impact in biodosimetry. Physiol Res 57:475–479Google Scholar
- Fernández-Gil B, Moneim AE, Ortiz F, Shen YQ, Soto-Mercado V, Mendivil-Perez M, Guerra-Librero A, Acuña-Castroviejo D, Molina-Navarro MM, García-Verdugo JM, Sayed RK, Florido J, Luna JD, López LC, Escames G (2017) Melatonin protects rats from radiotherapy-induced small intestine toxicity. PLoS One 12:e0174474CrossRefGoogle Scholar
- Montoro A, Barquinero JF, Almonacid M, Montoro A, Sebastià N, Verdú G, Sahuquillo V, Serrano J, Saiz M, Villaescusa JI, Soriano JM (2011) Concentration-dependent protection by ethanol extract of propolis against γ-ray-induced chromosome damage in human blood lymphocytes. Evid Based Complement Alternat Med 2011:174853–174859CrossRefGoogle Scholar
- Sun GW, Qiu ZD, Wang WN, Sui X, Sui DJ (2016) Flavonoids extraction from propolis attenuates pathological cardiac hypertrophy through PI3K/AKT signaling pathway. Evid Based Complement Alternat Med 2016:6281376Google Scholar
- Van Laethem A, Van Kelst S, Lippens S, Declercq W, Vandenabeele P, Janssens S, Vandenheede JR, Garmyn M, Agostinis P (2004) Activation of p38 MAPK is required for Bax translocation to mitochondria, cytochrome c release and apoptosis induced by UVB irradiation in human keratinocytes. FASEB J 18:1946–1948CrossRefGoogle Scholar
- Whitfield CD, Bostedor R, Goodrum D, Haak M, Chu EH (1981) Hamster cell mutants unable to grow on galactose and exhibiting an overlapping complementation pattern are defective in the electron transport chain. J Biol Chem 256:6651–6656Google Scholar