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Effect of NaCl on nitrification performance and extracellular polymeric substance characteristic of Klebsiella sp. TN-10

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Abstract

Klebsiella sp. TN-10, isolated from tannery wastewater, exhibited excellent nitrification ability cultivated in media with NaCl contents ranging from 0 to 35 g/L. Analysis of antioxidative enzyme activities of TN-10 stressed by NaCl showed that the stain produced more superoxide dismutase (SOD) and catalase (CAT) to fight against salt stress conditions. Moreover, the auto-aggregation index of TN-10 decreased with the increment of NaCl contents, which is consistent with results of zeta potentials. Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses showed that the compositions of extracellular polymeric substances (EPS) by TN-10 after NaCl shock were similar. Furthermore, proteins and polysaccharides were the main components of EPS, and the contents were greatly influenced by salinity. Then, circular dichroism (CD) spectrum was used to calculate the contents of each protein secondary structure. These results provided further insights into the knowledge of nitrification characteristics, auto-aggregation performance, and EPS compositions of TN-10 during NaCl shock.

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Acknowledgements

This study was funded by the Special Foundation for State Key Research and Development Program of China (2017YFB0308500), the National Natural Science Foundation of China (31871787), and Talent Training platform construction of Sichuan University (SCUKG017).

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Correspondence to Chongde Wu.

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Li, D., Jin, Y., Huang, J. et al. Effect of NaCl on nitrification performance and extracellular polymeric substance characteristic of Klebsiella sp. TN-10. Environ Sci Pollut Res 26, 24900–24910 (2019). https://doi.org/10.1007/s11356-019-05742-6

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Keywords

  • Salt-tolerant nitrifying bacteria
  • Auto-aggregation performance
  • NaCl shock
  • Extracellular polymeric substances
  • Extinction-emission matrix
  • Fourier-transform infrared