Environmental Science and Pollution Research

, Volume 26, Issue 20, pp 20815–20828 | Cite as

Modulation of lipid content and lipid profile by supplementation of iron, zinc, and molybdenum in indigenous microalgae

  • Madan Kumar Mandal
  • Papori Saikia
  • Ng.Kunjarani Chanu
  • Neha ChaurasiaEmail author
Research Article


The effects of iron (Fe), zinc (Zn), and molybdenum (Mo) on the biomass yield, lipid content, lipid yield, and fatty acid composition of Chlorella sp. NC-MKM, Graesiella emersonii NC-M1, Scenedesmus acutus NC-M2, and Chlorophyta sp. NC-M5 were studied. Among them, G. emersonii NC-M1 recorded the highest percentage increase in lipid content (140.3%) and neutral lipid (50.9%) under Zn-supplemented condition compared to the control. Also, it showed a 105% and 41.88% increase in lipid yield and neutral lipid under Fe-supplemented condition compared to the control. However, Chlorella sp. NC-MKM recorded an elevation in lipid yield (70.3% rise) and neutral lipid (24.32% rise) compared to the control in Mo-supplemented condition. The enhanced production of reactive oxygen species (ROS) and antioxidant enzyme (SOD and POD) under Fe-, Zn-, and Mo-supplemented condition supports the lipid accumulation. FAME analysis showed that the overall percentage of SFA and MUFA increased after the addition of Fe, Zn, and Mo in a culture medium compared to the control which is vital for a good-quality biodiesel. Further, biodiesel properties derived from FAMEs such as CN, SV, IV, CFPP, OS, υ, ρ, and HHV were found in accordance with biodiesel standard.


Biomass yield Lipid yield FAMEs Neutral lipid Biodiesel properties Oxidative stress 



The authors are thankful to the Head of Department, Biotechnology and Bioinformatics, North-Eastern Hill University for providing the necessary facilities. Also, the authors thank Guwahati Biotech Park, Assam, India, for the support in GC–MS analysis.

Funding information

This study received financial support from the Department of Biotechnology and Council of Scientific & Industrial Research, Government of India.

Supplementary material

11356_2019_5065_MOESM1_ESM.docx (166 kb)
Supplementary Figure 1 (DOCX 165 kb)
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Supplementary Figure 2 (DOCX 774 kb)
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Supplementary Figure 3 (DOCX 386 kb)
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Supplementary Figure 4 (DOCX 1179 kb)
11356_2019_5065_MOESM5_ESM.docx (11 kb)
Supplementary Table 1 (DOCX 11 kb)
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Supplementary Table 2 (DOCX 11 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Environmental Biotechnology laboratory, Department of Biotechnology and BioinformaticsNorth-Eastern Hill UniversityShillongIndia

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