Preparation and characterization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) microspheres for controlled release of buprofezin

  • Chan ZhangEmail author
  • Ruzhu Jia
  • Yuefeng Dong
  • Liangqi Zhao
Research Article


Extensive application of pesticides has caused a lot of environmental pollution and health problems, prompting the development of highly efficient and lowly toxic pesticide formulations. Here, buprofezin (BPF)-loaded poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-HH)) microspheres were prepared by O/W emulsion/solvent evaporation method. Under optimal conditions (P(HB-HH) 7.07% (w/v) and PVA 1.84% (w/v)), the spherical and monodispersed microspheres were obtained. The average particle size, pesticide loading (PL), and encapsulation efficiency (EE) of the optimized microspheres were 1.2 μm, 15.68%, and 78%, respectively. Release of 80% BPF from the microspheres in pH 5 (192 h) was faster than that in pH 7 (228 h) and 8 (204 h). Moreover, kinetic analysis indicated that BPF release behaved in a non-Fickian diffusion manner (0.43 < n < 0.85) and the release mechanism was the combined effects of pesticide diffusion and hydrolysis of polymer. The bioassay and toxicity results showed that encapsulation of BPF could exhibit high efficacy on the target organism and low toxicity to the non-target organism. Therefore, these results demonstrated that BPF-loaded P(HB-HH) microspheres with good stability were prepared successfully, and they could be further explored for constructing other highly efficient and lowly toxic pesticide formulations.


Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) Optimization Controlled release Buprofezin 


Funding information

This work was supported by the Science and Technology Program of Shanxi Province (201803D221002-1) and the Natural Science Foundation for Young Scientists of Shanxi Province, China (2013021011-7).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Chan Zhang
    • 1
    Email author
  • Ruzhu Jia
    • 1
  • Yuefeng Dong
    • 2
  • Liangqi Zhao
    • 3
  1. 1.School of Environment and SafetyTaiyuan University of Science and TechnologyTaiyuanChina
  2. 2.Shanxi Institute of Medicine and Life ScienceTaiyuanChina
  3. 3.Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of EducationShanxi UniversityTaiyuanChina

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