Biodegradation aspects of ibuprofen and identification of ibuprofen-degrading microbiota in an immobilized cell bioreactor
An enrichment process was employed by applying high ibuprofen concentration in an immobilized cell bioreactor in order to favor the ibuprofen-degrading community present in activated sludge. Experimental data showed the ability of the immobilized cell bioreactor to achieve high ibuprofen removal efficiencies (98.4 ± 0.3%), the tendency of the enriched biomass to acidify the treated liquor, and the inhibition of the nitrification process. Illumina sequencing revealed a massive increase in the relative abundance of Alphaproteobacteria and Gammaproteobacteria (from 29.1 to 80.8%) and a dramatic decrease in the proportion of Bacteroidetes, Planctomycetes, and Verrucomicrobia (from 42.7 to 2.1%) when pure ibuprofen served as the sole carbonaceous feeding substrate. This shift in the feeding conditions resulted in the predominance of Novosphingobium and Rhodanobacter (25.5 ± 10.8% and 25.2 ± 3.0%, respectively) and demonstrated a specialized ibuprofen-degrading bacterial community in activated sludge, which possessed the selective advantage to cope with its degradation. To the best of our knowledge, this bioreactor system was capable of effectively treating the highest ibuprofen concentration applied in wastewater treatment plants.
KeywordsIbuprofen-degrading bacteria Immobilized cell bioreactor Acidification Novosphingobium Rhodanobacter Non-steroidal anti-inflammatory drug (NSAID) degradation
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Chow J, Kovacic F, Dall Antonia Y, Krauss U, Fersini F, Schmeisser C, Lauinger B, Bongen P, Pietruszka J, Schmidt M, Menyes I, Bornscheuer UT, Eckstein M, Thum O, Liese A, Mueller-Dieckmann J, Jaeger KE, Streit WR (2012) The metagenome-derived enzymes LipS and LipT increase the diversity of known lipases. PLoS One 7(10):e47665CrossRefGoogle Scholar
- Clesceri LS, Greenberg AE, Eaton AD (1998) Standard methods for the examination of water and wastewater, 20th edn. American Public Health Association (APHA), Washington DCGoogle Scholar
- Corcoll N, Acuña V, Barceló D, Casellas M, Guasch H, Huerta B, Petrovic M, Ponsatí L, Rodríguez-Mozaz S, Sabater S (2014) Pollution-induced community tolerance to non-steroidal anti-inflammatory drugs (NSAIDs) in fluvial biofilm communities affected by WWTP effluents. Chemosphere 112:185–193CrossRefGoogle Scholar
- Islas-Flores H, Manuel Gómez-Oliván L, Galar-Martínez M, Michelle Sánchez-Ocampo E, SanJuan-Reyes N, Ortíz-Reynoso M, Dublán-García O (2017) Cyto-genotoxicity and oxidative stress in common carp (Cyprinus carpio) exposed to a mixture of ibuprofen and diclofenac. Environ Toxicol 32(5):1637–1650CrossRefGoogle Scholar
- Jallouli N, Pastrana-Martínez LM, Ribeiro AR, Moreira NFF, Faria JL, Hentati O, Silva AMT, Ksibi M (2018) Heterogeneous photocatalytic degradation of ibuprofen in ultrapure water, municipal and pharmaceutical industry wastewaters using a TiO2/UV-LED system. Chem Eng J 334:976–984CrossRefGoogle Scholar
- Langenhoff A, Inderfurth N, Veuskens T, Schraa G, Blokland M, Kujawa-Roeleveld K, Rijnaarts H (2013) Microbial removal of the pharmaceutical compounds ibuprofen and diclofenac from wastewater. BioMed Res Int. https://doi.org/10.1155/2013/325806
- Li Z, Cheng K, Teng X, Ma X, Hou Y, Wei Y (2015) Degradation spectrum of ibuprofen degrading strains. Acta Sci Circumst 35(1):177–183Google Scholar
- Masella AP, Bartram AK, Truszkowski JM, Brown DG, Neufeld JD (2012) PANDAseq: paired-end assembler for illumina sequences. BMC Bioinformatics 13(31). https://doi.org/10.1186/1471-2105-13-31
- Nalin R, Simonet P, Vogel TM, Normand P (1999) Rhodanobacter lindaniclasticus gen. nov., sp. nov., a lindane-degrading bacterium. Int J Syst Evol Microbiol 49(1):19–23Google Scholar