Environmental Science and Pollution Research

, Volume 26, Issue 11, pp 11371–11386 | Cite as

Generation of biodiesel from industrial wastewater using oleaginous yeast: performance and emission characteristics of microbial biodiesel and its blends on a compression injection diesel engine

  • Anbarasan TamilalaganEmail author
  • Jayanthi Singaram
  • Sakthivel Rajamohan
Research Article


Microbial-derived biodiesel was tested on a lab scale CI diesel engine for carrying out exhaust emission and performance characteristics. The performance, emission, and combustion characteristics of a single cylinder four stroke fixed compression ratio engine when fueled with microbial bio-diesel and its 10–30% blends with diesel (on a volume basis) were investigated and compared with conventional diesel. The bio-diesel was obtained from microbes which were grown by combining distillery spent wash with lignocellulosic hydrolysate at nutrient deprived conditions. The microbes consumed the wastes and converted the high strength waste water into lipids, which were trans-esterified to form bio-diesel. Testing of microbial bio-diesel blends with ordinary diesel at different loading pressures and the emission characteristics were compared. Results indicate that with increasing of the blends, reduction of HC and CO emissions were observed, whilst brake thermal efficiency maxed out at 20% blending. Further increase of blends showed a tendency of increasing of both emissions in the exhaust stream. The Brake Specific Fuel consumption was observed to decline with blending until 20% and then increased. The nitrogen oxide emissions, however, were found to increase with increasing blend ratios and reached a maximum at 20% blend. The escalation of HC, CO, CO2, and NOx emissions was also observed at higher blending ratios and higher engine loads. The performance studies were able to show that out of the three blends of biodiesel, 20% biodiesel blend was able to deliver the best of reduced hydrocarbon and carbon monoxide emissions, whilst also delivering the highest Brake thermal efficiency and the lowest Brake Specific Fuel consumption.


Microbial biodiesel Biodiesel blends Emission reduction Thermal efficiency Specific fuel consumption 



Compression direct ignition




Brake thermal efficiency


Brake specific fuel consumption


Brake mean effective pressure


Fatty acid methyl esters


American Society for Testing and Materials


Distillery spent wash


Lignocellulosic biomass hydrolysate


Compression direct injection


Fourier transform infra-red spectroscopy


Tri-acyl glycerol


Microbial type culture and collection


Conventional diesel fuel


Carbon monoxide


Carbon dioxide


Nitrogen oxides



The authors would like to thank Centre of excellence for environmental studies in Government College of Technology for funding this research. We also thank the Department of Mechanical Engineering, GCT, for running tests using CDI engine.


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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Government College of TechnologyCoimbatoreIndia
  2. 2.Government College of EngineeringBodinayakkanurIndia
  3. 3.Department of Mechanical EngineeringAmrita Vishwa PeethamCoimbatoreIndia

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