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Pyrene degradation by marine-derived ascomycete: process optimization, toxicity, and metabolic analyses

  • Maria R. S. Vasconcelos
  • Gabriela A. L. Vieira
  • Igor V. R. Otero
  • Rafaella C. Bonugli-Santos
  • Marili V. N. Rodrigues
  • Vera L. G. Rehder
  • Milene Ferro
  • Sinésio Boaventura
  • Maurício BacciJr
  • Lara D. SetteEmail author
Research Article
  • 59 Downloads

Abstract

Marine-derived fungi are relevant genetic resources for bioremediation of saline environments/processes. Among the five fungi recovered from marine sponges able to degrade pyrene (Py) and benzo[a]pyrene (BaP), Tolypocladium sp. strain CBMAI 1346 and Xylaria sp. CBMAI 1464 presented the best removal rates of Py and BaP, respectively. Since the decrease in BaP was related to mycelial adsorption, a combined strategy was applied for the investigation of Py degradation by the fungus Tolypocladium sp. CBMAI 1346. The selected fungus was able to degrade about 95% of Py after 7 days of incubation (optimized conditions), generating metabolites different from the ones found before optimization. Metabolites and transcriptomic data revealed that the degradation occurred mainly by the cytochrome P450 pathway. Putative monooxygenases and dioxygenases found in the transcriptome may play an important role. After 21 days of degradation, no toxicity was found in the optimized culture conditions. The findings from the present study highlight the potential of marine-derived fungi to degrade environmental pollutants and convey innovative information related to the metabolism of pyrene.

Keywords

PAH degradation Artemia Experimental design Marine biotechnology Transcriptome 

Notes

Acknowledgments

MRSV thanks the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (no. 2011/18769-3) and Coordenação de Aperfeiçoamento Pessoal de Nível Superior (CAPES) for her scholarships. GALV thanks the FAPESP (no. 2018/03372-0) for her technical grant. IVRO thanks the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (no. 170714/2017-9) for his scholarship. LDS thanks the CNPq for her Productivity Fellowship (303145/2016-1). The authors would like to thank Lucas Miotelo for the assistance with the article images.

Funding information

This study was financed by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (grant nos. 2013/19486-0 and 2016/07957-7).

Supplementary material

11356_2019_4518_MOESM1_ESM.pdf (85 kb)
ESM 1 (PDF 84 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Maria R. S. Vasconcelos
    • 1
  • Gabriela A. L. Vieira
    • 2
  • Igor V. R. Otero
    • 2
  • Rafaella C. Bonugli-Santos
    • 1
    • 3
  • Marili V. N. Rodrigues
    • 4
  • Vera L. G. Rehder
    • 4
  • Milene Ferro
    • 5
  • Sinésio Boaventura
    • 4
  • Maurício BacciJr
    • 2
    • 5
  • Lara D. Sette
    • 1
    • 2
    Email author
  1. 1.Divisão de Recursos Microbianos, Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e AgrícolasUniversidade Estadual de CampinasPaulíniaBrazil
  2. 2.Departamento de Bioquímica e Microbiologia, Instituto de BiociênciasUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP)Rio ClaroBrazil
  3. 3.Latin American Institute of Life and Nature SciencesFederal University of Latin American Integration (UNILA)ParanáBrazil
  4. 4.Divisão de Química Orgânica e Farmacêutica, Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e AgrícolasUniversidade Estadual de CampinasPaulíniaBrazil
  5. 5.Centro de Estudos de Insetos Sociais, Instituto de BiociênciasUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP)Rio ClaroBrazil

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