Daphniopsis tibetana Sars lives in elevation, usually with strong solar UV radiation. We speculate that UV may have an effect on the ecology and evolutionary biology of this species. However, the regulatory effect and mechanism of UV on D. tibetana have not been studied previously. Here, our results showed that UVB could act as a positive factor in the relative body lengths, reproductive parameters, and population growth parameters of D. tibetana when UVB radiation is 20–170 mJ cm−2, compared with the control group. Strikingly, these parameters were highest at 120 mJ cm−2. To explore the mechanism underlying the UVB irradiation effects, we conducted a transcriptome analysis using the Trinity platform. The results indicated that differentially regulated genes were mostly enriched in lipid transport and lipid localization by Gene Ontology (GO) enrichment analysis of 146 differentially expressed genes (83 upregulated and 63 downregulated). This is the first study of UVB radiation of D. tibetana to reveal genes that may have crucial roles in survival, growth, and reproduction and could be candidates for future functional studies. Additionally, the study could supply a substantial resource for investigating and elucidating lipids that could play important roles in a physiological context.
Daphniopsis tibetana Sars UVB radiation Survival and growth Development Reproductive parameters Transcriptome analysis
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We thank Robbie Lewis, MSc, from Liwen Bianji, Edanz Group China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.
W. Z. and M. R. W. conceived and designed the experiments. The experiments were performed by M. R. W., W. Z., J. W., S. W., and X.X. Data was analyzed by W. Z. and M. R. W. The paper was written by M. R. W. and W. Z.
This work was supported by a grant from the National Natural Science Foundation of China (No. 41501535).
Abdi H (2007) Bonferroni and Šidák corrections for multiple comparisons. Encyclopedia of Measurement and Statistics 3:103–107Google Scholar
Arts M, Rai H (1997) Effects of enhanced ultraviolet-B radiation on the production of lipid, polysaccharide and protein in three freshwater algal species. Freshwater Biol 38:597–610CrossRefGoogle Scholar
Berrendero F, Garcia-Gil L, Hernandez ML, Romero J, Cebeira M, de Miguel R et al (1998) Localization of mRNA expression and activation of signal transduction mechanisms for cannabinoid receptor in rat brain during fetal development. Development 125:3179–3188Google Scholar
Dytham C (2011) Choosing and using statistics: a biologist’s guide. Wiley-Blackwell, OxfordGoogle Scholar
Eads BD, Colbourne JK, Bohuski E, Andrews J (2007) Profiling sex-biased gene expression during parthenogenetic reproduction in Daphnia pulex. BMC Genomics 8:1–14CrossRefGoogle Scholar
Gruijl FR, Kranen HJ, Mullenders LH (2001) UV-induced DNA damage, repair, mutations and oncogenic pathways in skin cancer. J Photochem Photobiol B Biol 63:19–27CrossRefGoogle Scholar
Ichihashi M, Ueda M, Budiyanto A, Bito T, Oka M, Fukunaga M, Tsuru K, Horikawa T (2003) UV-induced skin damage. Toxicology 189:21–39CrossRefGoogle Scholar
Jokinen IE, Markkula ES, Salo HM, Kuhn P, Nikoskelainen S, Arts MT, Browman HI (2008) Exposure to increased ambient ultraviolet B radiation has negative effects on growth, condition and immune function of juvenile Atlantic salmon (Salmo salar). Photochem Photobiol 84:1265–1271CrossRefGoogle Scholar
Lange H, Donk E (1997) Effects of UVB-irradiated algae on life history traits of Daphnia pulex. Freshwater Biol 38:711–720CrossRefGoogle Scholar
Lange HJ, Reeuwijk PL (2003) Negative effects of UVB-irradiated phytoplankton on life history traits and fitness of Daphnia magna. Freshwater Biol 48:678–686CrossRefGoogle Scholar
Lopo AB, Spyrides MHC, Lucio PS, Sigró J (2013) Ozone and aerosol influence on ultraviolet radiation on the east coast of the Brazilian Northeast. Atmos Climate Sci 4:92–99Google Scholar
Marionnet C, Pierrard C, Golebiewski C, Bernerd F (2014) Diversity of biological effects induced by longwave UVA rays (UVA1) in reconstructed skin. PLoS One 9:e105263CrossRefGoogle Scholar
Maverakis E, Miyamura Y, Bowen MP, Correa G, Ono Y, Goodarzi H (2010) Light, including ultraviolet. J Autoimmun 34:247–257CrossRefGoogle Scholar
Morgan J, Iwama G (1991) Effects of salinity on growth, metabolism, and on regulation in juvenile rainbow and steel2head trout and fall chinook salmon. Can J Fish Aquat Sci 8:2083–2094CrossRefGoogle Scholar
Pfeifer GP, Besaratinia A (2012) UV wavelength-dependent DNA damage and human non-melanoma and melanoma skin cancer. Photochem Photobiol Sci 11:90–97CrossRefGoogle Scholar
Pfundt R, van Vlijmen-Willems I, Bergers M, Wingens M, Cloin W, Schalkwijk J (2001) In situ demonstration of phosphorylated c-jun and p38 MAP kinase in epidermal keratinocytes following ultraviolet B irradiation of human skin. J Pathol 193:248–255CrossRefGoogle Scholar
Rainuzzo JR, Reitan KI, Olsen Y (1997) The significance of lipids at early stages of marine fish: a review. Aquaculture 155:103–115CrossRefGoogle Scholar
Seah NE, Magalhaes Filho CD, Petrashen AP, Henderson HR, Laguer J, Gonzalez J et al (2016) Autophagy-mediated longevity is modulated by lipoprotein biogenesis. Autophagy 12:261–272CrossRefGoogle Scholar
Shi M, Lin Y, Xu G, Xie L, Hu X, Bao Z, Zhang R (2013) Characterization of the Zhikong scallop (Chlamys farreri) mantle transcriptome and identification of biomineralization-related genes. Mar Biotechnol 15:706–715CrossRefGoogle Scholar
Singh H, Rao BJ, Chary KV (2014) 1 H, 13 C and 15 N NMR assignments of a mutant of UV inducible transcript (S55A-UVI31+) from Chlamydomonas reinhardtii. Biomol NMR Assign 8:371–374CrossRefGoogle Scholar
Storey JD (2003) The positive false discovery rate: a Bayesian interpretation and the q-value. Ann Stat 31:2013–2035CrossRefGoogle Scholar
Su W, Wei SS, Hu SQ, Tang JX (2009) Preparation of TiO2/Ag colloids with ultraviolet resistance and antibacterial property using short chain polyethylene glycol. J Hazard Mater 172:716–720CrossRefGoogle Scholar
Sun X, Yang A, Wu B, Zhou L, Liu Z (2015) Characterization of the mantle transcriptome of Yesso scallop (Patinopecten yessoensis): identification of genes potentially involved in biomineralization and pigmentation. PLoS One 10:e0122967CrossRefGoogle Scholar
Tartarotti B, Cabrera S, Psenner R, Sommaruga R (1999) Survivorship of Cyclops abyssorum tatricus (Cyclopoida, Copepoda) and Boeckella gracilipes (Calanoida, Copepoda) under ambient levels of solar UVB radiation in two high-mountain lakes. J Plankton Res 21:549–560CrossRefGoogle Scholar
Touyz RM, Schiffrin EL (2000) Signal transduction mechanisms mediating the physiological and pathophysiological actions of angiotensin II in vascular smooth muscle cells. Pharmacol Rev 52:639–672Google Scholar
Webb AR, Kline L, Holick MF (1988) Influence of season and latitude on the cutaneous synthesis of vitamin D3: exposure to winter sunlight in Boston and Edmonton will not promote vitamin D3 synthesis in human skin. J Clin Endocrinol Metab 67:373–378CrossRefGoogle Scholar
Xu L, Zhao W, Xu X, Wei J, Shi X (2007) Effect of ultraviolet radiation on growth, development and reproduction of Moina mongolica Daday. J Agric Biotechnol 15:789–792 (In Chinese)Google Scholar
Zellmer ID (1996) The impact of food quantity on UV-B tolerance and recovery from UV-B damage in Daphnia pulex. Hydrobiologia 319:87–92CrossRefGoogle Scholar
Zhang P, Li C, Zhu L, Su X, Li Y, Jin C, Li T (2013) De novo assembly of the sea cucumber Apostichopus japonicus hemocytes transcriptome to identify miRNA targets associated with skin ulceration syndrome. PLoS One 8:e73506CrossRefGoogle Scholar
Zhang YN, Zhu XY, Wang WP, Wang Y, Wang L, Xu XX, Zhang K, Deng DG (2016) Reproductive switching analysis of Daphnia similoides between sexual female and parthenogenetic female by transcriptome comparison. Sci Rep 6:34241CrossRefGoogle Scholar
Zhao W, Huo Y, Gao J (2006) Analysis and appraisement of nutrient compositions for Daphniopsis tibetana Sars. J Fish Sci China 13:446–451 (In Chinese)Google Scholar
Zhao L, Liu L, Wang S, Wang H, Jiang J (2016a) Transcriptome profiles of metamorphosis in the ornamented pygmy frog Microhyla fissipes clarify the functions of thyroid hormone receptors in metamorphosis. Sci Rep 6:27310CrossRefGoogle Scholar
Zhao W, Zhao YY, Wang QH, Zhen MP, Wei J, Wang S (2016b) The community structure and seasonal dynamics of plankton in Bange Lake, northern Tibet, China. Chin J Oceanol Limnol 34:1143–1157CrossRefGoogle Scholar
Zhao W, Huo YZ, Zhang TM, Wang S, Shi TT (2017a) Effects of lithium on the survival, growth, and reproduction of Daphniopsis tibetana Sars (Crustacea: Cladocera). Chin J Oceanol Limnol 35:754–762CrossRefGoogle Scholar
Zhao W, You ZX, Wei J, Wang S (2017b) Compensatory population growth in Daphniopsis tibetana Sars (Crustacea: Cladocera) following starvation. Limnology 18:167–174CrossRefGoogle Scholar