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L-α-Phosphatidylcholine attenuates mercury-induced hepato-renal damage through suppressing oxidative stress and inflammation

  • Samar S. Elblehi
  • Mona H. Hafez
  • Yasser S. El-SayedEmail author
Research Article
  • 41 Downloads

Abstract

The potential ameliorative effects of L-α-phosphatidylcholine (PC) against mercuric chloride (HgCl2)-induced hematological and hepato-renal damage were investigated. Rats were randomly allocated into four groups (n = 12): control, PC (100 mg/kg bwt, intragastrically every other day for 30 consecutive days), HgCl2 (5 mg/kg bwt, intragastrically daily), and PC plus HgCl2. Hematological and hepato-renal dysfunctions were evaluated biochemically and histopathologically. Hepatic and renal oxidative/antioxidative indices were evaluated. The expression of proinflammatory cytokines (tumor necrosis factor-α and interleukin-6) was also detected by ELISA. HgCl2 significantly increased serum aminotransferases (ALT, AST), urea, and creatinine levels that are indicative of hepato-renal damage. HgCl2 also induced a significant accumulation of malondialdehyde (+ 195%) with depletion of glutathione (− 43%) levels in the liver and renal tissues. The apparent hepato-renal oxidative damage was associated with obvious organ dysfunction that was confirmed by impairments in the liver and kidney histoarchitecture. Furthermore, HgCl2 significantly attenuated the expression of proinflammatory cytokines named tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Conversely, PC treatment attenuated these effects, which improved the hematological and serum biochemical alternations, reduced the oxidative stress and proinflammatory cytokine levels, and ameliorated the intensity of the histopathological alterations in livers and kidneys of HgCl2-treated rats. It could be concluded that PC displayed potential anti-inflammatory and antioxidant activities against HgCl2-induced hepato-renal damage via suppression of proinflammatory cytokines and declining oxidative stress.

Keywords

Phosphatidylcholine Mercury Antioxidants Proinflammatory cytokines Histopathology 

Abbreviations

ALT

alanine aminotransferase

ANOVA

analysis of variance

AST

aspartate aminotransferase

DTNB

5,5-dithiobis (2-nitrobenzoic acid)

ELISA

enzyme-linked immunosorbent assay

GSH

reduced glutathione

Hb

hemoglobin

Hg

mercury

HgCl2

mercuric chloride

IL-6

interleukin-6

MCH

mean corpuscle concentration

MCHC

mean corpuscle hemoglobin concentration

MCV

mean corpuscle volume

MDA

malondialdehyde

PC

L-α-phosphatidylcholine

PCV

packed cell volume

RBC

red blood corpuscles

ROS

reactive oxygen species

SEM

standard error of the mean

TBARS

thiobarbituric acid-reactive substances

TNF-α

tumor necrosis factor-α

WBC

white blood corpuscles

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Pathology, Faculty of Veterinary MedicineAlexandria UniversityEdfinaEgypt
  2. 2.Department of Physiology, Faculty of Veterinary MedicineAlexandria UniversityEdfinaEgypt
  3. 3.Department of Forensic Medicine and Toxicology, Faculty of Veterinary MedicineDamanhur UniversityDamanhourEgypt

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