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Fine particulate matter alters the microecology of the murine respiratory tract

  • Biao Yang
  • Yu Zhang
  • Bingyu Li
  • Yang Zou
  • Chunling XiaoEmail author
Research Article
First Online:
  • 6 Downloads

Abstract

Fine particulate matter is a global challenge to human health. We investigated the effects and potential mechanisms of fine particulate matter on respiratory tract microecology in a lung injury mouse model. BALB/c mice were randomized into exposed and control groups. We found that the levels of soluble tumor necrosis factor receptor I was increased following the PM2.5 exposure. 16S rRNA sequencing of respiratory tract lavage fluid confirmed that the composition of the respiratory tract microecology was altered by the exposure. Lactobacillus was the most abundant of bacterial species present. Collectively, these results establish a link between exposure to fine particulate matter and alterations to the respiratory tract microecology. Elucidation of the underlying mechanisms may lead to treatment strategies in lung injury.

Keywords

PM2.5 Lung injury Microecology Respiratory tract Mouse 

Abbreviations

PM2.5

fine particulate matter

rRNA

ribosomal RNA

H&E

hematoxylin and eosin

EtOH

ethanol

PCR

polymerase chain reaction

RDP classifier

Ribosomal Database Project classifier

OTU

operational taxonomic unit

PCA

principal component analysis

PCoA

principal coordinates analysis

UPGMA

unweighted pair-group method with arithmetic means

sTNF-RI

soluble tumor necrosis factor type I

KEGG

Kyoto Encyclopedia of Genes and Genomes

TNF

tumor necrosis factor

kD

kilodalton

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Notes

Author contributions statement

BY and CX contributed conception and design of the study; BY carried out all experimental assays. YZ and BL performed the statistical analysis; BY wrote the first draft of the manuscript; BY, YZ, YZ, and BL wrote sections of the manuscript. All authors contributed to manuscript revision and read and approved the submitted version.

Funding information

The work was supported by the research funding from the National Natural Science Foundation of China (grant no. 30872083) and Liaoning Science and Technology Project (grant no. 2017225076).

Compliance with ethical standards

All studies were performed according to protocols reviewed and approved by the Ethics Committee of Animal Care and Experimentation of the National Institute for Environmental Studies, China.

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Lab of Environmental Pollution and Microecology of Liaoning ProvinceShenyang Medical CollegeShenyangPeople’s Republic of China

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