Environmental Science and Pollution Research

, Volume 26, Issue 8, pp 7804–7809 | Cite as

Evaluation of the antifungal activity of individual and combined monoterpenes against Rhizopus stolonifer and Absidia coerulea

  • Lijun Zhou
  • Zhilin Zhang
  • Mi Wei
  • Yongjian Xie
  • Shan He
  • Hongan Shi
  • Zhufeng LinEmail author
Research Article


The development of natural plant extracts and essential oils will help to decrease the negative effects of synthetic chemicals. In the present study, the antifungal activity of individual and combined monoterpenes against Rhizopus stolonifer and Absidia coerulea was evaluated. The results from antifungal tests showed that eugenol, carvacrol, and isoeugenol, among all the tested compounds, exhibited strong antifungal activity against the two tested fungi. Furthermore, carvacrol exhibited the most toxic effects against R. stolonifer and A. coerulea, and the IC50 values of carvacrol for the two fungi were 44.94 μg/ml and 50.83 μg/ml, respectively. The compounds (±)-menthol, b-citronellol, geraniol, 3,7-dimethyl-1-octanol, citral, and cuminaldehyde had only strong antifungal activity against R. stolonifer. In addition, the value of the synergistic co-efficient (SR) of a combination of isoeugenol and eugenol (1:1) showed an additive effect against R. stolonifer. The combination of isoeugenol and cuminaldehyde (1:1) showed an antagonistic effect against A. coerulea. Our results indicated that carvacrol and isoeugenol had potential antifungal effects against the two tested fungi and could be utilized in novel biological fungicide development.


Antifungal activity Carvacrol Isoeugenol Cuminaldehyde Additive effect Antagonistic effect 


Funding information

This study was financially supported by the Educational Commission of Hubei Province (No. B2016185), the National Fund (No. 31600055), and the Hubei Province Fund (No. 2018CFB675).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Lijun Zhou
    • 1
  • Zhilin Zhang
    • 2
  • Mi Wei
    • 2
  • Yongjian Xie
    • 3
  • Shan He
    • 2
  • Hongan Shi
    • 2
  • Zhufeng Lin
    • 4
    Email author
  1. 1.College of Plant ProtectionAnhui Agricultural UniversityHefeiPeople’s Republic of China
  2. 2.Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, College of Life Science and TechnologyHubei Engineering UniversityXiaoganPeople’s Republic of China
  3. 3.School of Agricultural and Food ScienceZhejiang A & F UniversityLinanPeople’s Republic of China
  4. 4.Key Laboratory of Plant Diseases and Insect Pests Control of HainanInstitute of Plant Protection of Hainan Academy of Agricultural SciencesHaikouPeople’s Republic of China

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