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Strategic growth of limnic green microalgae with phycoremediation potential for enhanced production of biomass and biomolecules for sustainable environment

  • Pandian Sureshkumar
  • Jibu Thomas
Advances in chemical and bio process engineering
  • 24 Downloads

Abstract

The approach of combining the microalgae cultivation with wastewater provides a cost-effective and eco-friendly perspective in the production of microalgae-based bio-products. In the present investigation, microalgae Scenedesmus rubescens KACC 2 isolated from catchment region of River Noyyal was found to be efficient in removing nitrogen, phosphorus, and heavy metals from industrial and domestic effluents, which was optimized through central composite design matrix for higher biomass generation. Nutrient requirements for the growth were optimized and evaluated using Plackett–Burman design to check the effect of variables. Three variables, viz., nitrate, phosphate, and inoculums, were found to be significant among the 11 variables tested, and the interaction between these variables and its optimum concentrations were statistically studied using central composite design matrix. The optimized growth conditions of this strain were found to be as nitrate (0.2%), phosphate (0.018%), and inoculums (7.5%). These conditions yielded a higher biomass of 0.73 g/L from the optimized media which was 5.4 times higher than the regular growth media. FT-IR analysis showed the variations in the spectra and also in biomolecular composition with 2-fold increase in the lipid and protein region when grown in optimized culture conditions. Lipid profile showed the presence of saturated and monounsaturated fatty acids in the biomass accepting it as a source of energy feedstock. This study concludes that nitrate, phosphate, and inoculums play a significant role in biomass production of S. rubescens with phycoremediation potential that can be exploited for simultaneous wastewater treatment–coupled biomass production.

Keywords

Microalgae Phycoremediation Response surface methodology Optimization Biomass Biomolecules 

Notes

Acknowledgments

The authors thank the Karunya Institute of Technology and Sciences for providing the research facilities. The authors also thank Dr. Murugan S. and Ms. Femin S. Uthap for critically evaluating the manuscript.

Funding information

This study received financial support funded by the Science and Engineering Research Board–Department of Science and Technology (SERB–DST), Government of India (S.O: No/SB/FT/LS-389/2012)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Algae Biomass Research Laboratory, Department of Biosciences and TechnologyKarunya Institute of Technology and Sciences (Deemed to be University)CoimbatoreIndia

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