1H NMR-based serum metabolomics analysis of the age-related metabolic effects of perinatal exposure to BPA, BPS, BPF, and BPAF in female mice offspring
- 65 Downloads
The widespread application of bisphenols (BPs) in the industry has made them ubiquitous in the environment, causing potential environmental risks. Its unknown impacts on human being have received more and more attention. In this study, we have assessed the metabolic effects of perinatal exposure to bisphenol A (BPA) and its substitutes (bisphenol S (BPS), bisphenol F (BPF), and bisphenol AF (BPAF)) in female mice adolescent offspring and female mice adulthood offspring. 1H NMR-based serum metabolomics showed that metabolic profiles were disturbed with BPA and its three substitutes exposure in female mice adolescent offspring and female mice adulthood offspring. In addition, age-related metabolic effects were found based on changes in serum endogenous metabolites and metabolic pathways. Specifically, metabolic pathway analysis showed that major disturbed metabolic pathways in female mice adulthood offspring compare with female mice adolescent offspring also changed significantly. With the increase of age of the female mice offspring, changes in the metabolic pathways became more obvious in the BPA treatment group. Conversely, partially disturbed metabolic pathways were restored in the BPS, BPF, and BPAF treatment groups. In conclusion, perinatal exposure to BPA and its three substitutes significantly interferes with metabolic profiles and metabolic pathways, and this metabolic effects were age-related. These results offer more detailed information about the age-related metabolic effects of perinatal exposure to BPA, BPS, BPF, and BPAF in female mice offspring and provide data for systematic evaluation of the health risk assessment of BPA and its substitutes.
KeywordsBPA Substitutes Age-related Metabolomics
We gratefully acknowledge the financial support from National Key Research and Development Program of China (2016YFD0200202), and the Young Elite Scientists Sponsorship Program by CAST.
Compliance with ethical standards
All experimental procedures were in accordance with the Institute of Zoology Animal and Medical Ethics Committee of China Agriculture University.
- Chen S, Zhang M, Bo L, Li S, Hu L, Zhao X, Sun C (2017) Metabolomic analysis of the toxic effect of chronic exposure of cadmium on rat urine. Environ Sci Pollut Res 1:1–10Google Scholar
- Fiege H, Voges HW, Hamamoto T, Umemura S, Iwata T, Miki H, Fujita Y, Buysch HJ, Garbe D, Paulus W (2000) Phenol derivatives. Ullmann's Encyclopedia of Industrial Chemistry. Wiley, WeinheimGoogle Scholar
- Gronwald W, Klein MS, Kaspar H et al (2008) Urinary metabolite quantification employing 2D NMR spectroscopy[J]. Anal Chem 80(23):9288–9297Google Scholar
- Kohlmeier M (2015) Nutrient metabolism: structures, functions, and genes. Elsevier, AmsterdamGoogle Scholar
- Pryor JL, Hughes C, Foster W, Hales BF, Robaire B (2000) Critical windows of exposure for children’s health: the reproductive system in animals and humans. Environ Health Perspect 108:491–503Google Scholar
- Rebuli ME, Cao J, Sluzas E, Delclos KB, Camacho L, Lewis SM, Vanlandingham MM, Patisaul HB (2014) Investigation of the effects of subchronic low dose oral exposure to bisphenol A (BPA) and ethinyl estradiol (EE) on estrogen receptor expression in the juvenile and adult female rat hypothalamus. Toxicol Sci An Off J Soc Toxicol 140:190–203CrossRefGoogle Scholar
- Rocha BA, Da CB, de Albuquerque NC, de Oliveira AR, Souza JM, Altameemi M, Campiglia AD, Jr BF (2016) A fast method for bisphenol A and six analogues (S, F, Z, P, AF, AP) determination in urine samples based on dispersive liquid-liquid microextraction and liquid chromatography-tandem mass spectrometry. Talanta 154:511–519CrossRefGoogle Scholar
- Rochester JR, Bolden A, Bisphenol SF (2015) A systematic review and comparison of the hormonal activity of bisphenol a substitutes[J]. Environ Health Perspect 123(7):643–50Google Scholar