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Uptake of copper from acid mine drainage by the microalgae Nannochloropsis oculata

  • Maria del Rosario Martínez-Macias
  • Ma. A. Correa-Murrieta
  • Yedidia Villegas-Peralta
  • Germán Eduardo Dévora-Isiordia
  • Jesús Álvarez-Sánchez
  • Jorge Saldivar-Cabrales
  • Reyna G. Sánchez-Duarte
Research Article
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Abstract

The removal of heavy metals from acid mine drainage is a key factor for avoiding damage to the environment. The microalga Nannochloropsis oculata was cultured in an algal medium with 0.05, 0.1, 0.15, 0.2, and 0.25 mM copper under completely defined conditions to assess its removal capacity; the effects of copper on the cell density and lipid productivity of N. oculata were also evaluated. The results showed that N. oculata was able to remove up to 99.92 ± 0.04% of the copper content in the culture medium. A total of 89.29 ± 1.92% was eliminated through metabolism, and 10.70 ± 1.92% was removed by adsorption. These findings are favorable because they indicate that a large amount of copper was extracted due to the ability of the microalga to metabolize copper ions. The cell density, growth rate, and lipid content decreased with increased concentrations of copper in the culture medium. A positive effect on the fatty acid profile was found, as the saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA) content improved when the copper concentration was higher than 0.1 mmol L−1, which can potentiate the production of high-quality biodiesel. N. oculata is a good option for the treatment of acid mine drainage due to its ability to eliminate a substantial percentage of the copper present. Moreover, combining different culture systems such that heavy metals are removed to non-toxic levels in the first stage and high cell densities, which promote lipid production, is obtained in the second stage would be an advantageous strategy.

Keywords

Microalgae Lipids Biodiesel Heavy metals Acid mine drainage 

Notes

Acknowledgements

This work was supported by the Instituto Tecnológico de Sonora through project promotion and supporting research development (PROFAPI, 2016).

References

  1. AOAC (2005) Official methods of analysis of AOAC International, official methods of analysis. Association of official analytical chemist, international, Gaithersburg, Maryland, EUAGoogle Scholar
  2. Çetinkaya Dönmez G, Aksu Z, Öztürk A, Kutsal T (1999) A comparative study on heavy metal biosorption characteristics of some algae. Process Biochem 34:885–892CrossRefGoogle Scholar
  3. Chan A, Salsali H, McBean E (2014) Heavy metal removal (copper and zinc) in secondary effluent from wastewater treatment plants by microalgae. ACS Sustain Chem Eng 2:130–137CrossRefGoogle Scholar
  4. Das BK, Roy A, Koschorreck M, Mandal SM, Wendt-Potthoff K, Bhattacharya J (2009) Occurrence and role of algae and fungi in acid mine drainage environment with special reference to metals and sulfate immobilization. Water Res 43:883–894CrossRefGoogle Scholar
  5. Davies KL, Davies MS, Francis D (1991) Zinc-induced vacuolation in root meristematic cells of Festuca rubra L. Plant Cell Environ 14:399–406CrossRefGoogle Scholar
  6. Fabregas J, Abalde J, Herrero C, Cabezas B, Veiga M (1984) Growth of the marine microalga Tetraselmis suecica in batch cultures with different salinities and nutrient concentrations. Aquaculture 42:207–215CrossRefGoogle Scholar
  7. Folch J, Lees M, Sloane-Stanley G (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509Google Scholar
  8. Francisco ËC, Jacob-Lopes E, Neves DB, Franco TT (2009) Microalgae as feedstock for biodiesel production: carbon dioxide sequestration, lipid production and biofuel quality. New Biotechnol 25(Supplement):S278–S279CrossRefGoogle Scholar
  9. Fripp A, Stermer R, Catlin A (1970) Structure of silicon films deposited on oxidized silicon wafers. J Electrochem Soc 117:1569–1571CrossRefGoogle Scholar
  10. Gadapati WR, Macfie SM (2006) Phytochelatins are only partially correlated with Cd-stress in two species of Brassica. Plant Sci 170:471–480CrossRefGoogle Scholar
  11. Garcia-Sanchez A, Alvarez-Ayuso E (2003) Arsenic in soils and waters and its relation to geology and mining activities (Salamanca Province, Spain). J Geochem Explor 80:69–79CrossRefGoogle Scholar
  12. Guedes AC, Amaro HM, Malcata FX (2011) Microalgae as sources of high added-value compounds—a brief review of recent work. Biotech Prog 27:597–613CrossRefGoogle Scholar
  13. Hirata K, Tsuji N, Miyamoto K (2005) Biosynthetic regulation of phytochelatins, heavy metal-binding peptides. J Biosci Bioeng 100:593–599CrossRefGoogle Scholar
  14. Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M, Darzins A (2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J 54:621–639CrossRefGoogle Scholar
  15. Khairy H (2009) Toxicity and accumulation of copper in Nannochloropsis oculata (Eustigmatophyceae, Heterokonta). World Appl Sci J 6:378–384Google Scholar
  16. Lee KY, Mooney DJ (2012) Alginate: properties and biomedical applications. Prog Polym Sci 37:106–126CrossRefGoogle Scholar
  17. Liao Q, Chang H-X, Fu Q, Huang Y, Xia A, Zhu X, Zhong N (2018) Physiological-phased kinetic characteristics of microalgae Chlorella vulgaris growth and lipid synthesis considering synergistic effects of light, carbon and nutrients. Bioresource Technology 250:583–590CrossRefGoogle Scholar
  18. Maksymiec W (1998) Effect of copper on cellular processes in higher plants. Photosynthetica 34:321–342CrossRefGoogle Scholar
  19. Martínez-Macías R, Meza-Escalante E, Serrano-Palacios D, Gortáres-Moroyoqui P, Ruíz-Ruíz PE, Ulloa-Mercado G (2018) Effect of fed-batch and semicontinuous regimen on Nannochloropsis oculata grown in different culture media to high-value products. J Chem Technol Biotechnol 93:585–590CrossRefGoogle Scholar
  20. Martinoia E, Klein M, Geisler M, Sánchez-Fernández R, Rea P (2000) Vacuolar transport of secondary metabolites and xenobiotics. Annu Plant Rev 5:221–253Google Scholar
  21. Richards RG, Mullins BJ (2013) Using microalgae for combined lipid production and heavy metal removal from leachate. Ecol Model 249:59–67CrossRefGoogle Scholar
  22. Rodolfi L, Chini Zittelli G, Bassi N, Padovani G, Biondi N, Bonini G, Tredici MR (2009) Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng 102:100–112CrossRefGoogle Scholar
  23. Romera E, González F, Ballester A, Blázquez ML, Muñoz JA (2007) Comparative study of biosorption of heavy metals using different types of algae. Bioresour Technol 98:3344–3353CrossRefGoogle Scholar
  24. Salt DE, Rauser WE (1995) MgATP-dependent transport of phytochelatins across the tonoplast of oat roots. Plant Physiol 107:1293–1301CrossRefGoogle Scholar
  25. Sánchez-Machado DI, Núñez-Gastélum JA, Reyes-Moreno C, Ramírez-Wong B, López-Cervantes J (2010) Nutritional quality of edible parts of Moringa oleifera. Food Anal Method 3:175–180CrossRefGoogle Scholar
  26. Scholz MJ, Weiss TL, Jinkerson RE, Jing J, Roth R, Goodenough U, Posewitz MC, Gerken HG (2014) Ultrastructure and composition of the Nannochloropsis gaditana cell wall. Eukaryot Cell 13:1450–1464CrossRefGoogle Scholar
  27. Sharma SS, Dietz K-J, Mimura T (2016) Vacuolar compartmentalization as indispensable component of heavy metal detoxification in plants. Plant Cell Environ 39:1112–1126CrossRefGoogle Scholar
  28. Sukarni S, Hamidi N, Yanuhar U, Wardana ING (2014) Potential and properties of marine microalgae Nannochloropsis oculata as biomass fuel feedstock. Int J Energ Environ Eng 5:279–290CrossRefGoogle Scholar
  29. Yang J, Cao J, Xing G, Yuan H (2015) Lipid production combined with biosorption and bioaccumulation of cadmium, copper, manganese and zinc by oleaginous microalgae Chlorella minutissima UTEX2341. Bioresource Technol 175:537–544CrossRefGoogle Scholar
  30. Zeraatkar AK, Ahmadzadeh H, Talebi AF, Moheimani NR, McHenry MP (2016) Potential use of algae for heavy metal bioremediation, a critical review. J Environ Manag 181:817–831CrossRefGoogle Scholar
  31. Zhu CJ, Lee YK (1997) Determination of biomass dry weight of marine microalgae. J Appl Phycol 9:189–194CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Maria del Rosario Martínez-Macias
    • 1
  • Ma. A. Correa-Murrieta
    • 1
  • Yedidia Villegas-Peralta
    • 1
  • Germán Eduardo Dévora-Isiordia
    • 1
  • Jesús Álvarez-Sánchez
    • 1
  • Jorge Saldivar-Cabrales
    • 1
  • Reyna G. Sánchez-Duarte
    • 1
  1. 1.Departamento de Ciencias del Agua y Medio AmbienteInstituto Tecnológico de SonoraCiudad ObregónMéxico

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