Sensitivity of physiological and biochemical endpoints in early ontogenetic stages of crops under diclofenac and paracetamol treatments
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Early stages of ontogenesis determining subsequent growth, development, and productivity of crops can be affected by wastewater and sludge contaminated with pharmaceuticals. Diclofenac (DCF) and paracetamol (PCT; both 0.0001 to 10 mg/L) did not affect seed germination and primary root length of onion, lettuce, pea, and tomato. Conversely, 20-day-old pea and maize plants exhibited decrease in biomass production, leaf area (by approx. 40% in pea and 70% in maize under 10 mg/L DCF), or content of photosynthetic pigments (by 10% and 60% under 10 mg/L PCT). Quantum yields of photosystem II were reduced only in maize (FV/FM and ΦII by more than 40% under 10 mg/L of both pharmaceuticals). Contents of H2O2 and superoxide increased in roots of both species (more than four times under 10 mg/L PCT in pea). Activities of antioxidant enzymes were elevated in pea under DCF treatments, but decreased in maize under both pharmaceuticals. Oxidative injury of root cells expressed as lowered oxidoreductase activity (MTT assay, by 40% in pea and 80% in maize) and increase in malondialdehyde content (by 60% and 100%) together with the membrane integrity disruption (higher Evans Blue accumulation, by 100% in pea and 300% in maize) confirmed higher sensitivity of maize as a C4 monocot plant to both pharmaceuticals.
KeywordsNonsteroidal anti-inflammatory drugs Crop plants Growth Content of pharmaceuticals Oxidative stress
This work was supported by the Czech Science Foundation (CZ) and FWF Der Wissenschaftsfonds (AT) project no. GF 17-33746L (I 3046- N28).
- Bałdyga B, Wieczorek J, Smoczyński S, Wieczorek Z, Smoczyńska K (2005) Pea plant response to anthracene present in soil. Pol J Environ Stud 14:397–401Google Scholar
- Copolovici L, Timis D, Taschina M, Copolovici D, Cioca G, Bungau S (2017) Diclofenac influence on photosynthetic parameters and volatile organic compounds emission from Phaseolus vulgaris L plants. Rev Chim-Bucharest 68:2076–2078Google Scholar
- Corcoll N, Acuña V, Barceló D, Casellas M, Guasch H, Huerta B, Petrovic M, Ponsatí L, Rodríguez-Mozaz S, Sabater S (2014) Pollution-induced community tolerance to non-steroidal anti-inflammatory drugs (NSAIDs) in fluvial biofilm communities affected by WWTP effluents. Chemosphere 112:185–193CrossRefGoogle Scholar
- García-Limones C, Hervás A, Navas-Cortés JA, Jiménez-Díaz RM, Tena M (2002) Induction of an antioxidant enzyme system and other oxidative stress markers associated with compatible and incompatible interactions between chickpea (Cicer arietinum L.) and Fusarium oxysporum f. sp. ciceris. Physiol Mol Plant Pathol 61:325–337CrossRefGoogle Scholar
- Jin Z, Pei Y (2015) Physiological implications of hydrogen sulfide in plants: pleasant exploration behind its unpleasant odour. Oxidative Med Cell Longev 2015:397502Google Scholar
- Kalaji HM, Rastogi A (2017) Pharmaceutical compounds: an emerging pollutant (a review on plant-pharmaceuticals interaction). Chiang Mai J Sci 44:287–297Google Scholar
- Linder G, Greene J, Ratsch H, Nwosu J, Smith S, Wilborn D (1989) Seed germination and root elongation toxicity tests in hazardous waste site evaluation: methods development and applications. U.S Environmental Protection Agency Washington D.C, EPA/600/D-89/109 NTIS PB90113184Google Scholar
- Piotrowicz-Cieślak AI, Adomas B, Nalęcz-Jawecki G (2012) Phytotoxicity of sulfonamide soil pollutants to legume plant species. Fresenius Environ Bull 21:1316–1320Google Scholar
- Zhu HS, Chen X, Pan XL, Zhang DY (2011) Effects of chloramphenicol on pigmentation, proline accumulation and chlorophyll fluorescence of maize (Zea mays) seedlings. Int J Agric Biol 13:677–682Google Scholar
- Ziólkowska A, Piotrowicz-Cieślak AI, Rydzyński D, Adomas B, Nalęcz-Jawecki G (2014) Biomarkers of leguminous plant viability in response to soil contamination with diclofenac. Pol J Environ Stud 23:263–269Google Scholar
- Ziólkowska A, Piotrowicz-Cieślak AI, Margas M, Adomas B, Nalęcz-Jawecki G (2015) Accumulation of tetracycline oxytetracycline and chlortetracycline in pea (Pisum sativum L.). Fresenius Environ Bull 24:1386–1391Google Scholar