Environmental Science and Pollution Research

, Volume 26, Issue 5, pp 5065–5075 | Cite as

Effects of sludge lysate for Cr(VI) bioreduction and analysis of bioaugmentation mechanism of sludge humic acid

  • Hongling Chen
  • Ruofei JinEmail author
  • Guangfei Liu
  • Tian Tian
  • Chen Gu
  • Jiti Zhou
  • Defeng XingEmail author
Research Article


This study evaluated the effects of sludge lysate (SL) on the anaerobic bioreduction of Cr(VI) and the role of sludge humic acid (SHA) during this process. The results showed that supplement of SL significantly enhanced the efficiency of Cr(VI) bioreduction by 29.61%, in 12 h compared with that of the control without SL. Moreover, SHA exhibited promoting effects on bioreduction of Cr(VI), and the promotion increased with increasing SHA concentrations from 100 to 300 mg/L. In the presence of 300 mg/L SHA, Cr(VI) (98.21 mg/L) was completely reduced after 24 h with a removal rate increased by 34.3% compared with that of the control without SHA. Further investigation on the bioaugmentation mechanism of SHA by studying the nature of SHA and the reaction mechanism between SHA and Cr(VI) revealed that SHA exhibited a strong adsorption ability, which could adsorb and combine with Cr(VI). The adsorption capacity of Cr(VI) by SHA was calculated as 34.4 mg/g with 0.2 g of SHA and 10 mg/L of Cr(VI). It could also act as redox mediators to accelerate the electron transfer between microorganisms and Cr(VI) to promote reduction of Cr(VI). Furthermore, the effects of SL on the microbial community compositions of the anaerobic Cr(VI) bioreduction system were studied. Brachymonas was the primary bacteria at the genus level. The abundance of electroactive bacteria, such as Acinetobacter, Pseudomonas, and Arcobacter, increased in the SL-amended system. These findings expand the versatility of SL and justify wider use of residual activated sludge, which might contribute to the treatment of heavy metal–contaminated wastewater.


Cr(VI) Bioreduction Sludge lysate Sludge humic acid Bioaugmentation 


Funding information

This study was financially supported by the Open Project of State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (no. HCK201811).

Supplementary material

11356_2018_3917_MOESM1_ESM.doc (24.4 mb)
ESM 1 (DOC 25028 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, School of Environmental Science and TechnologyDalian University of TechnologyDalianChina
  2. 2.State Key Laboratory of Urban Water Resource and EnvironmentHarbin Institute of TechnologyHarbinChina
  3. 3.Drainage Management OfficeTongliao Municipal Commission of Housing Urban-Rural DevelopmentTongliaoChina

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