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Environmental Science and Pollution Research

, Volume 26, Issue 3, pp 2761–2770 | Cite as

Bioconversion of lignin into bioplastics by Pandoraea sp. B-6: molecular mechanism

  • Dan Liu
  • Xu Yan
  • Mengying Si
  • Xinhui Deng
  • Xiaobo Min
  • Yan ShiEmail author
  • Liyuan ChaiEmail author
Research Article
  • 173 Downloads

Abstract

Lignin is a byproduct in the pulp and paper industry and is considered as a promising alternative for the provision of energy and chemicals. Currently, the efficient valorization of lignin is a challenge owing to its polymeric structure complexity. Here, we present a platform for bio-converting Kraft lignin (KL), to polyhydroxyalkanoate (PHA) by Pandoraea sp. B-6 (hereafter B-6). Depolymerization of KL by B-6 was first confirmed, and > 40% KL was degraded by B-6 in the initial 4 days. Characterization of PHA showed that up to 24.7% of PHA accumulated in B-6 grown in 6-g/L KL mineral medium. The composition, structure, and thermal properties of the produced PHA were analyzed, revealing that 3-hydroxybutyrate was the only monomer and that PHA was comparable with the commercially available bioplastics. Moreover, the genomic analysis illustrated three core enzymatic systems for lignin depolymerization including laccases, peroxidases, and Fenton-reaction enzymes; five catabolic pathways for LDAC degradation and a gene cluster consisting of bktB, phaR, phaB, phaA, and phaC genes involved in PHA biosynthesis. Accordingly, a basic model for the process from lignin depolymerization to PHA production was constructed. Our findings provide a comprehensive perspective for lignin valorization and bio-material production from waste.

Keywords

Lignin valorization Polyhydroxyalkanoate Pandoraea sp. B-6 Genomic analysis Five catabolic pathways Bio-material production 

Notes

Funding information

This work was supported by the National Key R&D Plan (2016YFC0403003), the National Natural Science Foundation of China (31400115, 51474102), and the China Postdoctoral Science Foundation (2017M612594).

Supplementary material

11356_2018_3785_MOESM1_ESM.doc (845 kb)
ESM 1 (DOC 845 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Environmental Science and Engineering, School of Metallurgy and EnvironmentCentral South UniversityChangshaChina
  2. 2.Chinese National Engineering Research Center for Control & Treatment of Heavy Metal PollutionChangshaChina

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