Environmental Science and Pollution Research

, Volume 26, Issue 3, pp 2671–2680 | Cite as

Long-term exposure to low level of fluoride induces apoptosis via p53 pathway in lymphocytes of aluminum smelter workers

  • Pingjing Wen
  • Xiaomin Wei
  • Guiqiang Liang
  • Yanfei Wang
  • Yiping Yang
  • Lilin Qin
  • Weiyi Pang
  • Guangqiu Qin
  • Hai Li
  • Yueming JiangEmail author
  • Qijun WuEmail author
Research Article


Long-term occupational exposure to low level of fluoride can induce oxidative stress and apoptosis in many cells, including lymphocyte. However, the underlying mechanism remains unclear. Hence, this study was designed to explore the potential oxidative stress and apoptosis of long-term occupational exposure to low level of fluoride in aluminum smelter workers. A total of 120 aluminum smelter workers were recruited in control, low-, middle-, and high-fluoride exposure groups with 30 workers for each group. The peripheral blood samples were collected, centrifuged, and isolated to obtain serum and lymphocyte suspensions. The air and serum fluoride concentrations were detected by fluoride ion-selective electrode method. The lymphocytic apoptosis rate, DNA damage, oxidative stress, and mRNA levels of p53, Bcl-2, and Bax were assessed by Annexin V/PI staining, comet assay, attenuated total reflectance Fourier transform infrared spectroscopy and real-time polymerase chain reaction, respectively. Results showed that the air and serum fluoride concentrations of fluoride-exposed groups were higher than those of the control group (p < 0.05). Fluoride exposure might induce apoptosis, DNA damage and oxidative stress in a dose-dependent manner in lymphocytes (p < 0.05). The expression levels of p53 and Bax were increased with fluoride exposure in lymphocytes (p < 0.05), whereas the Bcl-2 expression was decreased but not significantly. Taken together, these observations indicate that long-term occupational exposure to low level of fluoride may lead to oxidative stress and induce apoptosis through the p53-dependent pathway in peripheral blood lymphocytes of aluminum smelter workers. Serum fluoride level may be the potential biomarker of fluoride exposure.


Fluoride Apoptosis DNA damage Oxidative stress p53-dependent pathway 



The authors would like to thank the volunteers who participated in this study.

Funding information

This work was financially sponsored by the Scientific Research Foundation of the Education Department of Guangxi Province (Grant No. 2002316 and KY2015ZD058) and the National Natural Science Foundation (Grant No. 81602888).

Compliance with ethical standards

Conflict of interest statement

The authors declare that they have no conflicts of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Pingjing Wen
    • 1
    • 2
  • Xiaomin Wei
    • 1
  • Guiqiang Liang
    • 3
  • Yanfei Wang
    • 4
  • Yiping Yang
    • 1
  • Lilin Qin
    • 1
  • Weiyi Pang
    • 5
  • Guangqiu Qin
    • 3
  • Hai Li
    • 3
  • Yueming Jiang
    • 1
    Email author
  • Qijun Wu
    • 3
    Email author
  1. 1.Department of Toxicology, School of Public HealthGuangxi Medical UniversityNanningPeople’s Republic of China
  2. 2.Department of ToxicologyGuangxi Center for Disease Prevention and ControlNanningPeople’s Republic of China
  3. 3.Department of Preventive Medicine, School of Public Health and ManagementGuangxi University of Chinese MedicineNanningPeople’s Republic of China
  4. 4.Primary Care DivisionMaternal and Child Health HospitalChongqingPeople’s Republic of China
  5. 5.Department of Occupational and Environmental HealthGuilin Medical UniversityGuilinPeople’s Republic of China

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