Response of the antioxidant enzymes of rats following oral administration of metal-oxide nanoparticles (Al 2O 3, CuO, TiO 2) Research Article First Online: 12 November 2018 Abstract
Metal-oxide nanoparticles (NPs), as a new emerging technological compound, promise a wide range of usage areas and consequently have the potential to cause environmental toxicology. In the present work, aluminum (Al
2O 3), copper (CuO), and titanium (TiO 2) nanoparticles (NPs) were administered via oral gavage to mature female rats ( Rattus norvegicus var. albinos) for 14 days with a dose series of 0 (control), 0.5, 5, and 50 (mg/kg b.w./day). Enzyme activities of the antioxidant system such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST), and glutathione reductase (GR) in the liver were measured. Transmission electron microscope (TEM) images of the liver were taken to demonstrate NP accumulation and distribution in liver tissue. Data showed that all NPs caused some significant ( P > 0.05) alterations in the activities of antioxidant enzymes. CAT activity increased after CuO and TiO 2 administrations, while SOD activity decreased after Al 2O 3 administration. The activities of enzymes associated with glutathione (GR, GPx, GST) metabolisms were also significantly altered by NPs. GPx activity increased in rats received Al 2O 3, CuO NPs, while GR activity increased only by Al 2O 3. However, there were increases (TiO 2) and decreases (CuO) in GST activity in the liver of rats. TEM images of the liver demonstrated that all NPs accumulated in the liver, even at the lowest dose. This study indicated that the antioxidant enzymes in the liver of rats were affected by all NPs, suggesting the antioxidant system of rats suffered after NP administration. Keywords Metal Nanoparticle Rat Liver Antioxidant Biomarker
Responsible editor: Philippe Garrigues
This study was produced from PhD Thesis of Dr. E.G. Canli, except nanoparticle characterizations and supported by the research fund (FDK-2017-8197) of Cukurova University (Turkey). We thank Dr. G. Atli for her help.
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