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Environmental Science and Pollution Research

, Volume 25, Issue 36, pp 36530–36544 | Cite as

Reduction in arsenic toxicity and uptake in rice (Oryza sativa L.) by As-resistant purple nonsulfur bacteria

  • Phitthaya Nookongbut
  • Duangporn Kantachote
  • Mallavarapu Megharaj
  • Ravi Naidu
Research Article
  • 56 Downloads

Abstract

This study aimed to investigate the potential of Rhodopseudomonas palustris C1 and Rubrivivax benzoatilyticus C31 to ameliorate As toxicity and to reduce As uptake in rice. Strain C1 was superior to strain C31 for siderophore production. The mixed culture (1: 1) was most effective in reducing the toxicity of As species [As(III) and/or As(V), each 30 mg/l] by yielding maximal germination index that related to α- and β-amylase activities in two Thai rice cultivars (HomNil: HN and PathumThani 1: PT). Arsenic toxicity to the seed germination followed the order: mixed As species > As(III) > As(V); and the toxicity was reduced in inoculated sets, particularly with a mixed culture. The mixed culture significantly enhanced rice growth under As stress in both rice cultivars as indicated by an increase in the production of chlorophyll a and b, and also supporting the non-enzymatic (carotenoids, lipid oxidation, and nitric oxide) and enzymatic (superoxide dismutase, ascorbate peroxidase, catalase, and glutathione reductase) activities. These were concomitant with productions of 5-aminolevulinic acid, indole-3-acetic acid, exopolymeric substances, and siderophores which significantly reduced As accumulation in treated rice. It can be concluded that the mixed culture has great potential to ameliorate rice from As toxicity by preventing As species entry into rice for enhancing rice growth and also for reducing As accumulation to produce safe rice from rice grown in contaminated paddy fields.

Keywords

Arsenic Contamination Plant growth-promoting substances Phototrophic bacteria Protection mechanisms Rice 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11356_2018_3568_MOESM1_ESM.docx (2.3 mb)
ESM 1 (DOCX 2400 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Phitthaya Nookongbut
    • 1
  • Duangporn Kantachote
    • 1
    • 2
  • Mallavarapu Megharaj
    • 3
    • 4
  • Ravi Naidu
    • 3
    • 4
  1. 1.Department of Microbiology, Faculty of SciencePrince of Songkla UniversityHat YaiThailand
  2. 2.Center of Excellence on Hazardous Substance Management (HSM)BangkokThailand
  3. 3.Global Centre for Environmental Remediation, Faculty of ScienceThe University of NewcastleCallaghanAustralia
  4. 4.Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC-CARE)The University of NewcastleCallaghanAustralia

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