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Environmental Science and Pollution Research

, Volume 26, Issue 3, pp 2363–2374 | Cite as

Research on the nitrogen transformation in rhizosphere of winter wheat (Triticum aestivum) under molybdenum addition

  • Xin Wen
  • Chengxiao HuEmail author
  • Xuecheng Sun
  • Xiaohu Zhao
  • Qiling Tan
Research Article

Abstract

Molybdenum (Mo), an essential microelement for plants, animals, and microorganisms, is reported can reduce soil nitrogen (N) residues and regulate plant root growth, but little is known about its effect on soil N transformation in plant-root region. A specially designed rhizobox was used in the present study to investigate the N processes in rhizosphere and non-rhizosphere soils of winter wheat applied with different rates of Mo fertilizer. (1) In the rhizosphere soil, pH values increased with increasing rates of Mo application, nitrate (NO3-N) accumulated at the rates of 0.15 and 0.3 mg Mo kg−1, potential denitrification activity (PDA) was significantly reduced by application of 0.15–1 mg Mo kg−1, and the copy numbers of narG and nosZ genes were increased by application of 0.15–1 mg Mo kg−1. (2) In the non-rhizosphere soil, NO3-N content decreased by application of 0.15–0.3 mg Mo kg−1, and narG gene abundance increased obviously by application of 0.3–1 mg Mo kg−1. (3) Soil pH, NO3-N, apparent nitrification rate (ANR), and nosZ gene abundance were significantly higher in rhizosphere than in non-rhizosphere soil. On the contrary, NH4+-N and total N, PDA, the abundance of AOB, and nirK and nirS genes were significantly higher in non-rhizosphere soil. The results indicated that the N transformations in rhizosphere and non-rhizosphere soils were differently affected by soil application of Mo fertilizer, and rhizosphere played a more important role in soil N cycle processes. The regulatory effects of Mo on these processes were to increase plant biomass and N uptake, promote the NO3-N accumulation in rhizosphere soil, and weaken the denitrification in both rhizosphere and non-rhizosphere soils.

Keywords

Molybdenum Rhizosphere Non-rhizosphere Nitrogen transformation Soil 

Abbreviations

Mo

Molybdenum

N

Nitrogen

PDA

Potential denitrification activity

ANR

Apparent nitrification rate

MBN

Microbial biomass nitrogen

SOM

Soil organic matter

TN

Soil total nitrogen

Notes

Acknowledgments

We would like to thank Prof. Jianming Xue, Prof. Ron McLaren, and Dr. Lei Zhong for the help on this manuscript.

Funding

The study was financially supported by the National Key Research and Development Program of China (2016YFD0200108) and Fundamental Research Funds for the Central Universities (2010PY025).

Supplementary material

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High Resolution Image (TIF 1061 kb)
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Supplementary Figure 2

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High Resolution Image (TIF 2335 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Xin Wen
    • 1
    • 2
  • Chengxiao Hu
    • 1
    • 2
    Email author
  • Xuecheng Sun
    • 1
  • Xiaohu Zhao
    • 1
  • Qiling Tan
    • 1
  1. 1.Department of Resource and Environment/Hubei Provincial Engineering Laboratory for New Fertilizers/Research Center of Trace ElementsHuazhong Agricultural UniversityWuhanChina
  2. 2.Key Laboratory of Horticultural Plant Biology (HZAU), MOEHuazhong Agricultural UniversityWuhanChina

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