β-Pinene moderates Cr(VI) phytotoxicity by quenching reactive oxygen species and altering antioxidant machinery in maize
We examined the possible role of monoterpene β-pinene in providing protection against Cr(VI) toxicity in maize (Zea mays). Treatment with β-pinene (10 μM) significantly alleviated Cr(VI) accumulation and recuperated Cr(VI) caused decline in root and coleoptile growth in maize. β-Pinene addition caused a decline in Cr(VI)-induced accumulation of superoxide anion, hydroxyl ion, hydrogen peroxide and confirmed by in-situ detection of ROS using histochemical localization. It suggested that the β-pinene quenches/neutralizes enhanced ROS generated under Cr(VI) exposure. β-Pinene also reduced Cr(VI)-induced electrolyte leakage, thereby suggesting its role in membrane stabilization. Further, β-pinene regulated the activity of scavenging enzymes, thereby suggesting a role in modulating Cr(VI)-induced oxidative damage. In conclusion, our results suggest that the addition of β-pinene has a protective role against Cr(VI) stress and provides resistance to maize against Cr(VI) toxicity.
KeywordsMonoterpenes Hexavalent chromium Oxidative damage Free radicals Stress amelioration
PM is thankful to University Grants Commission, New Delhi, India, for research fellowship.
- Farquharson KL (2017) Secrets of the forest: volatiles first discovered in pine trees propagate defense signals within and between plants. Plant Cell 29:1181–1182Google Scholar
- Kriegs B, Jansen M, Hahn K, Peisker H, Šamajová O, Beck M, Braun S, Ulbrich A, Baluška F, Schulz M (2010) Cyclic monoterpene mediated modulations of Arabidopsis thaliana phenotype: effect on the cytoskeleton and on the expression of selected genes. Plant Signal Behav 5(7):832–838CrossRefGoogle Scholar
- Lee K, Seo PJ (2014) Airborne signals from salt-stressed Arabidopsis plants trigger salinity tolerance in neighboring plants. Plant Signal Behav 9(3):e28392
- Misra HR, Fridovich I (1972) The univalent reduction of oxygen by reduced flavins and quinines. J Biol Chem 247:188–192Google Scholar
- Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar
- Pompella A, Maellaro E, Casini AF, Comporti M (1987) Histochemical detection of lipid peroxidation in the liver of bromobenzene-poisoned mice. Arch Amer J Pathol 129:295–301Google Scholar
- Riedlmeier M, Ghirardo A, Wenig M, Knappe C, Koch K, Georgii E, Dey S, Parker JE, Schnitzler J-P, Vlot C (2017) Monoterpenes support systemic acquired resistance within and between plants. Plant Cell 29:1440–1459Google Scholar
- Shahid M, Shamshad S, Rafiq M, Khalid S, Bibi I, Niazi NK, Dumat C, Rashid MI (2017) Chromium speciation, bioavailability, uptake, toxicity and detoxification in soil-plant system: a review. Chemosphere 178:513–533Google Scholar
- Stambulska UY, Bayliak MM, Lushchak VI (2018) Chromium(VI) toxicity in legume plants: modulation effects of rhizobial rymbiosis. BioMed Res Int 2018: article ID 8031213, 13 pagesGoogle Scholar