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Ferruginous bodies resolved by synchrotron XRF in a dog with peritoneal malignant mesothelioma

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Abstract

Mesothelioma is a malignant tumor mainly correlated to occupational asbestos exposure. Rare reports describe its occurrence also in animals, mainly linked to asbestos in the environment. Asbestos exposure is demonstrated by the appearance of characteristic histological hallmarks: asbestos containing ferruginous bodies that are iron-based structures forming around fibers and also other dust particles. Here we present a clinical case of a suspect of mesothelioma in the peritoneum of a dog with parallel histological observation of ferruginous bodies. To possibly correlate the dog tumor to environmental exposure, we performed X-ray fluorescence (XRF) analyses at two different synchrotrons to resolve the ferruginous bodies’ composition. While the histological examination diagnoses a tubulo-papillary mesothelioma, the XRF analyses show that ferruginous bodies contain Si particles, resembling formations of exogenous origin; however, the morphology is unlikely that of asbestos fibers. We speculate that the peritoneal mesothelioma of this dog could be related to environmental exposure to non-asbestos material.

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Acknowledgements

The authors acknowledge a grant from Friuli Venezia Giulia Region, Commissione Amianto FVG, 2015. They are grateful to Elettra and the Australian Synchrotron for granting beamtimes. Part of this research was undertaken on the XFM beamline at the Australian Synchrotron, Victoria, Australia. The authors are grateful to the Australian Synchrotron for providing the beamtime and to D. Paterson, D. Howard, M. de Jonge, and K. Spiers for technical support at the XFM beamline.

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Authors and Affiliations

  1. ULSS n.2 Marca Trevigiana. Dipartimento di Prevenzione - Servizi Veterinari di Asolo, Treviso, 31100, Italy

    Ernesto Pascotto

  2. Elettra - Sincrotrone Trieste S.C.p.A, Strada Statale 14 - km 163,5 in AREA Science Park, 34149 Basovizza, Trieste, Italy

    Alessandra Gianoncelli

  3. Servizio Diagnostica Veterinaria, University of Udine, 33100 Udine, Italy

    Carla Calligaro

  4. Department of Medical, Surgical, and Health Sciences, University of Trieste, 34149, Trieste, Italy

    Thomas Marcuzzo & Clara Rizzardi

  5. Institute for Maternal and Child Health, IRCCS Burlo Garofolo, 34137, Trieste, Italy

    Mauro Melato & Lorella Pascolo

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  1. Ernesto Pascotto
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  2. Alessandra Gianoncelli
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  3. Carla Calligaro
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  4. Thomas Marcuzzo
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  5. Mauro Melato
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  6. Clara Rizzardi
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  7. Lorella Pascolo
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Correspondence to Lorella Pascolo.

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Responsible editor: Philippe Garrigues

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Pascotto, E., Gianoncelli, A., Calligaro, C. et al. Ferruginous bodies resolved by synchrotron XRF in a dog with peritoneal malignant mesothelioma. Environ Sci Pollut Res 25, 35707–35714 (2018). https://doi.org/10.1007/s11356-018-3521-x

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  • DOI: https://doi.org/10.1007/s11356-018-3521-x

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