Environmental Science and Pollution Research

, Volume 25, Issue 35, pp 35672–35681 | Cite as

Magnetite nanoparticles coated with oleic acid: accumulation in hepatopancreatic cells of the mangrove crab Ucides cordatus

  • Hector Aguilar VitorinoEmail author
  • Priscila Ortega
  • Roxana Y. Pastrana Alta
  • Flavia Pinheiro Zanotto
  • Breno Pannia Espósito
Research Article


The field of nanotechnology had enormous developments, resulting in new methods for the controlled synthesis of a wide variety of nanoscale materials with unique properties. Efficient methods such as thermal decomposition for efficient size control have been developed in recent years for the synthesis of oleic acid (OA)-coated magnetite (Fe3O4) nanoparticles (MNP-OA). These nanostructures can be a source of pollution when emitted in the aquatic environment and could be accumulated by vulnerable marine species such as crustaceans. In this work, we synthesized and characterized MNP-OA of three different diameters (5, 8, and 12 nm) by thermal decomposition. These nanoparticles were remarkably stable after treatment with high affinity iron chelators (calcein, fluorescent desferrioxamine, and fluorescent apotransferrin); however, they displayed pro-oxidant activity after being challenged with ascorbate under two physiological buffers. Free or nanoparticle iron displayed low toxicity to four types of hepatopancreatic cells (E, R, F, and B) of the mangrove crab Ucides cordatus; however, they were promptly bioavailable, posing the risk of ecosystem disruption due to the release of excess nutrients.


Iron Magnetite Ucides cordatus Fluorescence Hepatopancreas Mangrove crab 


Funding information

This work was financially supported by CAPES and FAPESP (Brazilian government agencies).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Fundamental Chemistry, Institute of ChemistryUniversity of São PauloSão PauloBrazil
  2. 2.Institute of BiosciencesUniversity of São PauloSão PauloBrazil
  3. 3.Faculty of ScienceNational University of EngineeringLimaPeru

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