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Environmental Science and Pollution Research

, Volume 25, Issue 35, pp 35301–35311 | Cite as

Acute and chronic toxicity effects of acrylonitrile to the juvenile marine flounder Paralichthys olivaceus

  • Pengfei Lin
  • Jingjing Miao
  • Luqing Pan
  • Lei Zheng
  • Xiufen Wang
  • Yufei Lin
  • Jiangyue Wu
Research Article
  • 21 Downloads

Abstract

Acrylonitrile (ACN) spills in marine environment have the potential to cause ecological hazards and consequences, but currently little is known about the disruptive effects of ACN on marine organisms. In the present study, we investigated the lethal and sublethal effects of ACN on juvenile flounder Paralichthys olivaceus. The results showed that the 96-h LC50 of ACN to P. olivaceus juveniles was 6.07 mg/L. The fish were then exposed to different sublethal concentrations (0.1, 0.2, and 0.4 mg/L) of ACN for 28 days and then transferred to clean seawater and keep in clean seawater for 14 days to simulate the conditions of a spill incident. Biomarkers (EROD, GST, SOD, AChE activity, and levels of LPO and DNA alkaline unwinding) were tested in liver and brain. The weight gain rates and specific growth rate of juvenile marine flounder exposed to ACN (≥ 0.1 mg/L) for 28 days decreased significantly, indicating that ACN had an inhibitory effect on juvenile growth. Deformity of fish tails was observed on individuals exposed to the highest concentration (0.4 mg/L ACN) for 14 days, and the malformation rate was 38% after 28-day exposure. The present study provides the first evidence that ACN causes inhibition of AChE activity in fish brain. Furthermore, the results showed that ACN can significantly inhibit SOD activity and cause lipid peroxidation and DNA damage in fish brain. The results indicated that brain is more sensitive to ACN toxicity compared to liver and provides a suitable tissue for biomonitoring. The biomarkers measured during the depuration period showed that the effects caused by ACN were reversible when the exposure concentration was lower than 0.4 mg/L. These results highlight the adverse effects of ACN in brain of fish, which should be considered in environmental risk assessment. Biomarkers including AChE activity, LPO, and DNA damage of brain tissue should be included in fish bioassays for toxic effect assessment of ACN spills.

Keywords

Hazardous and noxious substances (HNS) Acrylonitrile Chronic toxicity DNA damage Neurotoxicity Paralichthys olivaceus 

Abbreviations

HNS

Hazardous and noxious substances

ACN

Acrylonitrile

EROD

7-Ethoxyresorufin O-deethylase

GST

Glutathione S-transferase

SOD

Superoxide dismutase

LPO

Lipid peroxidation levels

AChE

Acetylcholinesterase

MDA

Malondialdehyde

CEO

2-Cyanoethylene oxide

OH8dG

8-Hydroxy-2′-deoxyguanosine

DTNB

5,5-Dithiobis-2-nitrobenzoate

ATCh

Acetylthiocholine iodide

ROS

Reactive oxygen species

Notes

Acknowledgments

We thank the anonymous reviewers for comments on an earlier draft of the paper.

Funding information

Financial support was provided by the National Marine Hazard Mitigation Service, State Ocean Administration project of China (2015AA019).

Supplementary material

11356_2018_3430_MOESM1_ESM.docx (234 kb)
ESM 1 (DOCX 233 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.The Key Laboratory of Mariculture Ministry of EducationOcean University of ChinaQingdaoChina
  2. 2.Lab. of Environmental Physiology of Aquatic Animal, Fisheries CollegeOcean University of ChinaQingdaoChina
  3. 3.National Marine Hazard Mitigation ServiceState Ocean AdministrationBeijingChina

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