Advertisement

Environmental Science and Pollution Research

, Volume 25, Issue 35, pp 35064–35073 | Cite as

Interaction of ciprofloxacin with the activated sludge of the sewage treatment plant

  • Kan Wang
  • Doudou Gao
  • Jirong Xu
  • Lu Cai
  • Junrui Cheng
  • Zhenxun Yu
  • Zenghui Hu
  • Jie Yu
Research Article
  • 49 Downloads

Abstract

Interaction of ciprofloxacin with the activated sludge of the sewage treatment plant is of importance for the ciprofloxacin migration and risk control. More than 96.0% ciprofloxacin was removed through the sludge adsorption. The sludge surface charge varied little with ciprofloxacin since most ciprofloxacin was dissociated into the neutral one. No obvious shift was observed for the soluble carbohydrate concentration and composition with the addition of ciprofloxacin, indicating the weak interaction between the carbohydrates and ciprofloxacin. The introduction of ciprofloxacin resulted in a reduction of the soluble protein concentration, a marked increase of the extracellular protein fluorescence intensities, and a dramatic emergence of new extracellular proteins. The alteration of the proteins highlights the strong interaction between the extracellular proteins and ciprofloxacin, and the consequent integration of certain soluble proteins and original unextractable inner layer extracellular proteins into the extractable extracellular proteins. Different types of interactions are suggested to dominate between the extracellular proteins and the differently dissociated ciprofloxacin.

Keywords

Ciprofloxacin Activated sludge Extracellular proteins Carbohydrates Interaction 

Notes

Funding information

This work was supported by the National Natural Science Foundation of China (grant numbers 51408325 and 41401538), the Natural Science Foundation of Ningbo (grant number 2016A610093), the Science and Technology Planning Project of Ningbo, Zhejiang Province (grant numbers 2015C110001 and 2016C51035), and the K.C. Wong Magna Fund in Ningbo University.

Supplementary material

11356_2018_3413_MOESM1_ESM.docx (380 kb)
ESM 1 (DOCX 380 kb)

References

  1. Anand U, Kurup L, Mukherjee S (2012) Deciphering the role of pH in the binding of ciprofloxacin hydrochloride to bovine serum albumin. Phys Chem Chem Phys 14:4250–4258CrossRefGoogle Scholar
  2. Bryant CM, McClements DJ (1998) Molecular basis of protein functionality with special consideration of cold-set gels derived from heat-denatured whey. Trends Food Sci Tech 9:143–151CrossRefGoogle Scholar
  3. Chen W, Westerhoff P, Leenheer JA, Booksh K (2003) Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter. Environ Sci Technol 37:5701–5710CrossRefGoogle Scholar
  4. De Voogt P, Janex-Habibi ML, Sacher F, Puijker L, Mons M (2009) Development of a common priority list of pharmaceuticals relevant for the water cycle. Water Sci Technol 59:39–46CrossRefGoogle Scholar
  5. Dickinson E (1999) Adsorbed protein layers at fluid interfaces: interactions, structure and surface rheology. Colloids Surf B: Biointerfaces 15:161–176CrossRefGoogle Scholar
  6. Dorival-García N, Zafra-Gómez A, Navalón A, González-López J, Hontoria E, Vílchez JL (2013) Removal and degradation characteristics of quinolone antibiotics in laboratory-scale activated sludge reactors under aerobic, nitrifying and anoxic conditions. J Environ Manag 120:75–83CrossRefGoogle Scholar
  7. Ebert I, Bachmann J, Kühnen U, Küster A, Kussatz C, Maletzki D, Schlüter C (2011) Toxicity of the fluoroquinolone antibiotics enrofloxacin and ciprofloxacin to photoautotrophic aquatic organisms. Environ Toxicol Chem 30:2786–2792CrossRefGoogle Scholar
  8. Esparza-Soto M, Westerhoff P (2003) Biosorption of humic and fulvic acids to live activated sludge biomass. Water Res 37:2301–2310CrossRefGoogle Scholar
  9. Fick J, Söderström H, Lindberg RH, Phan C, Tysklind M, Larsson DGJ (2009) Contamination of surface, ground, and drinking water from pharmaceutical production. Environ Toxicol Chem 28:2522–2527CrossRefGoogle Scholar
  10. Frederick KK, Marlow MS, Valentine KG, Wand AJ (2007) Conformational entropy in molecular recognition by proteins. Nature 448:325–329CrossRefGoogle Scholar
  11. Golet EM, Xifra I, Siegrist H, Alder AC, Giger W (2003) Environmental exposure assessment of fluoroquinolone antibacterial agents from sewage to soil. Environ Sci Technol 37:3243–3249CrossRefGoogle Scholar
  12. Guan B, Yu J, Fu H, Guo M, Xu X (2012) Improvement of activated sludge dewaterability by mild thermal treatment in CaCl2 solution. Water Res 46:425–432CrossRefGoogle Scholar
  13. Hyland KC, Dickenson ERV, Drewes JE, Higgins CP (2012) Sorption of ionized and neutral emerging trace organic compounds onto activated sludge from different wastewater treatment configurations. Water Res 46:1958–1968CrossRefGoogle Scholar
  14. Jia A, Wan Y, Xiao Y, Hu J (2012) Occurrence and fate of quinolone and fluoroquinolone antibiotics in a municipal sewage treatment plant. Water Res 46:387–394CrossRefGoogle Scholar
  15. Jorand F, Boué-Bigne F, Block JC, Urbain V (1998) Hydrophobic/hydrophilic properties of activated sludge exopolymeric substances. Water Sci Technol 37:307–315CrossRefGoogle Scholar
  16. Li B, Zhang T (2010) Biodegradation and adsorption of antibiotics in the activated sludge process. Environ Sci Technol 44:3468–3473CrossRefGoogle Scholar
  17. Lindberg RH, Wennberg P, Johansson MI, Tysklind M, Andersson BAV (2005) Screening of human antibiotic substances and determination of weekly mass flows in five sewage treatment plants in Sweden. Environ Sci Technol 39:3421–3429CrossRefGoogle Scholar
  18. Lindberg RH, Olofsson U, Rendahl P, Johansson MI, Tysklind M, Andersson BAV (2006) Behavior of fluoroquinolones and trimethoprim during mechanical, chemical, and active sludge treatment of sewage water and digestion of sludge. Environ Sci Technol 40:1042–1048CrossRefGoogle Scholar
  19. Métivier R, Bourven I, Labanowski J, Guibaud G (2013) Interaction of erythromycin ethylsuccinate and acetaminophen with protein fraction of extracellular polymeric substances (EPS) from various bacterial aggregates. Environ Sci Pollut R 20:7275–7285CrossRefGoogle Scholar
  20. Michael I, Rizzo L, McArdell CS, Manaia CM, Merlin C, Schwartz T, Dagot C, Fatta-Kassinos D (2013) Urban wastewater treatment plants as hotspots for the release of antibiotics in the environment: a review. Water Res 47:957–995CrossRefGoogle Scholar
  21. Onbasli D, Aslim B (2009) Effects of some organic pollutants on the exopolysaccharides (EPSs) produced by some Pseudomonas spp. strains. J Hazard Mater 168:64–67CrossRefGoogle Scholar
  22. Rybacka A, Andersson PL (2016) Considering ionic state in modeling sorption of pharmaceuticals to sewage sludge. Chemosphere 165:284–293CrossRefGoogle Scholar
  23. Sheng G, Yu H (2006) Characterization of extracellular polymeric substances of aerobic and anaerobic sludge using three-dimensional excitation and emission matrix fluorescence spectroscopy. Water Res 40:1233–1239CrossRefGoogle Scholar
  24. Sheng G, Zhang M, Yu H (2008) Characterization of adsorption properties of extracellular polymeric substances (EPS) extracted from sludge. Colloids Surf B: Biointerfaces 62:83–90CrossRefGoogle Scholar
  25. Sheng GP, Yu HQ, Li XY (2010) Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: a review. Biotechnol Adv 28:882–894CrossRefGoogle Scholar
  26. Stone MJ (2001) NMR relaxation studies of the role of conformational entropy in protein stability and ligand binding. Accounts Chem Res 34:379–388CrossRefGoogle Scholar
  27. Taylor NM, Davies RH, Ridley A, Clouting C, Wales AD, Clifton-Hadley FA (2008) A survey of fluoroquinolone resistance in Escherichia coli and thermophilic Campylobacter spp. on poultry and pig farms in Great Britain. J Appl Microbiol 105:1421–1431CrossRefGoogle Scholar
  28. Van Doorslaer X, Dewulf J, Van Langenhove H, Demeestere K (2014) Fluoroquinolone antibiotics: an emerging class of environmental micropollutants. Sci Total Environ 500-501:250–269CrossRefGoogle Scholar
  29. Wijekoon KC, Hai FI, Kang J, Price WE, Guo W, Ngo HH, Nghiem LD (2013) The fate of pharmaceuticals, steroid hormones, phytoestrogens, UV-filters and pesticides during MBR treatment. Bioresour Technol 144:247–254CrossRefGoogle Scholar
  30. Xu W, Zhang G, Li X, Zou S, Li P, Hu Z, Li J (2007) Occurrence and elimination of antibiotics at four sewage treatment plants in the Pearl River Delta (PRD), South China. Water Res 41:4526–4534CrossRefGoogle Scholar
  31. Xu J, Sheng G, Ma Y, Wang L, Yu H (2013) Roles of extracellular polymeric substances (EPS) in the migration and removal of sulfamethazine in activated sludge system. Water Res 47:5298–5306CrossRefGoogle Scholar
  32. Xu Q, Li X, Ding R, Wang D, Liu Y, Wang Q, Zhao J, Chen F, Zeng G, Yang Q, Li H (2017) Understanding and mitigating the toxicity of cadmium to the anaerobic fermentation of waste activated sludge. Water Res 124:269–279CrossRefGoogle Scholar
  33. Yi K, Wang D, QiYang LX, Chen H, Sun J, An H, Wang L, Deng Y, Liu J, Zeng G (2017) Effect of ciprofloxacin on biological nitrogen and phosphorus removal from wastewater. Sci Total Environ 605-606:368–375CrossRefGoogle Scholar
  34. Yu J, Guo M, Xu X, Guan B (2014) The role of temperature and CaCl2 in activated sludge dewatering under hydrothermal treatment. Water Res 50:10–17CrossRefGoogle Scholar
  35. Zorita S, Martensson L, Mathiasson L (2009) Occurrence and removal of pharmaceuticals in a municipal sewage treatment system in the south of Sweden. Sci Total Environ 407:2760–2770CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Faculty of Architectural, Civil Engineering and EnvironmentNingbo UniversityNingboChina
  2. 2.Ningbo Rail Transit Group Co., LtdNingboChina

Personalised recommendations