Environmental Science and Pollution Research

, Volume 25, Issue 35, pp 35064–35073 | Cite as

Interaction of ciprofloxacin with the activated sludge of the sewage treatment plant

  • Kan Wang
  • Doudou Gao
  • Jirong Xu
  • Lu Cai
  • Junrui Cheng
  • Zhenxun Yu
  • Zenghui Hu
  • Jie YuEmail author
Research Article


Interaction of ciprofloxacin with the activated sludge of the sewage treatment plant is of importance for the ciprofloxacin migration and risk control. More than 96.0% ciprofloxacin was removed through the sludge adsorption. The sludge surface charge varied little with ciprofloxacin since most ciprofloxacin was dissociated into the neutral one. No obvious shift was observed for the soluble carbohydrate concentration and composition with the addition of ciprofloxacin, indicating the weak interaction between the carbohydrates and ciprofloxacin. The introduction of ciprofloxacin resulted in a reduction of the soluble protein concentration, a marked increase of the extracellular protein fluorescence intensities, and a dramatic emergence of new extracellular proteins. The alteration of the proteins highlights the strong interaction between the extracellular proteins and ciprofloxacin, and the consequent integration of certain soluble proteins and original unextractable inner layer extracellular proteins into the extractable extracellular proteins. Different types of interactions are suggested to dominate between the extracellular proteins and the differently dissociated ciprofloxacin.


Ciprofloxacin Activated sludge Extracellular proteins Carbohydrates Interaction 


Funding information

This work was supported by the National Natural Science Foundation of China (grant numbers 51408325 and 41401538), the Natural Science Foundation of Ningbo (grant number 2016A610093), the Science and Technology Planning Project of Ningbo, Zhejiang Province (grant numbers 2015C110001 and 2016C51035), and the K.C. Wong Magna Fund in Ningbo University.

Supplementary material

11356_2018_3413_MOESM1_ESM.docx (380 kb)
ESM 1 (DOCX 380 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Faculty of Architectural, Civil Engineering and EnvironmentNingbo UniversityNingboChina
  2. 2.Ningbo Rail Transit Group Co., LtdNingboChina

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