Effects of tapeworm infection on absorption and excretion of zinc and cadmium by experimental rats
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The main objective of this study was to determine how rat tapeworms affect the excretion of zinc and cadmium through rat feces. Male rats (Rattus norvegicus var. alba) were divided into four groups, and the experiment was conducted over a 6-week period. The control groups (00; 0T) were provided with a standard ST-1 rodent mixture and received 10.5 mg of Zn/week. Groups P0 and PT were fed a mixture supplemented with the hyperaccumulating plant Arabidopsis halleri at a dosage of 123 mg Zn/week and 2.46 mg Cd/week. Groups 0T and PT were infected with the rat tapeworm (Hymenolepis diminuta). Fecal samples were collected 24 h post exposure. Zinc and cadmium concentrations in rat feces were analyzed using inductively coupled plasma optical emission spectrometry. Tapeworm presence decreased the amount of metals excreted through the feces of the host throughout the entire experiment, with the exception of 1 week (control group). No statistically significant differences between zinc excretion rates in the control groups (00 and 0T) were detected at any time throughout the experiment. A statistically significant difference between zinc excretion rates (p < 0.05) in the exposed groups (P0 and PT) was detected in 2 of the 6 monitored weeks. Group PT excreted significantly less cadmium (p < 0.01) than group P0 did in three of the 6 weeks. Overall, our results indicate that tapeworms are able to influence the excretion of metals by their host. Tapeworms accumulate metals from intestinal contents. It is not clear whether tapeworms carry out this process before the host tissues absorb the metals from the intestines or the tapeworms accumulate metals excreted from the body of the host back to the intestines. Most likely, it is a combination of both phenomena.
KeywordsZinc Cadmium Tapeworm Excretion Feces Rat Hyperaccumulators
The authors gratefully acknowledge Brian Kavalir for his proofreading services.
This study was supported by the University-wide internal grant agency of the Czech University of Life Sciences Prague (CIGA), project no. 20182005.
Compliance with ethical standards
All experiments with laboratory animals were conducted in compliance with the current laws of the Czech Republic Act No 246/1992 Coll. on the Protection of Animals against Cruelty.
Conflict of interest
The authors declare that they have no conflict of interest.
- Brand von T (1973) Biochemistry of parasites. Academic press, BethesdaGoogle Scholar
- Brody T (1998) Zinc and copper. Nutritional biochemistry. Academic Press, San DiegoGoogle Scholar
- Brožová A, Jankovská I, Miholová D, Scháňková Š, Truněčková J, Langrová I, Kudrnáčová M, Vadlejch J (2015) Heavy metal concentrations in the small intestine of red fox (Vulpes vulpes) with and without Echinococcus multilocularis infection. Environ Sci Pollut Res 22:3175–3179. https://doi.org/10.1007/s11356-014-3733-7 CrossRefGoogle Scholar
- Isaure MP, Huguet S, Meyer CL, Castillo-Michel H, Testemale D, Vantelon D, Saumitou-Laprade P, Verbruggen N, Sarret G (2015) Evidence of various mechanisms of Cd sequestration in the hyperaccumulator Arabidopsis halleri, the non-accumulator Arabidopsis lyrata, and their progenies by combined synchrotron-based techniques. J Exp Bot 66:3201–3214. https://doi.org/10.1093/jxb/erv131 CrossRefGoogle Scholar
- Jankovská I, Miholová D, Bejček V, Vadlejch J, Šulc M, Száková J, Langrová I (2010a) Influence of parasitism on trace element contents in tissues of red fox (Vulpes vulpes) and its parasites Mesocestoides spp. (Cestoda) and Toxascaris leonina (Nematoda). Arch Environ Contam Toxicol 58:469–477. https://doi.org/10.1007/s00244-009-9355-2 CrossRefGoogle Scholar
- Jankovská I, Vadlejch J, Száková J, Miholová D, Kunc P, Knížková I, Čadková Z, Langrová I (2010c) Experimental studies on the cadmium accumulation in the cestode Moniezia expansa (Cestoda: Anoplocephalidae) and its final host (Ovis aries). Exp Parasitol 126:130–134. https://doi.org/10.1016/j.exppara.2010.04.010 CrossRefGoogle Scholar
- Jankovská I, Sloup V, Száková J, Langrová I, Sloup S (2016) How the tapeworm Hymenolepis diminuta affects zinc and cadmium accumulation in a host fed a hyperaccumulating plant (Arabidopsis halleri). Environ Sci Pollut Res 23:19126–19133. https://doi.org/10.1007/s11356-016-7123-1 CrossRefGoogle Scholar
- Jankovská I, Sloup V, Száková J, Magdálek J, Nechybová S, Peřinková P, Langrová I (2018) How tapeworm infection and consumption of a Cd and Zn hyperaccumulating plant may affect Cu, Fe, and Mn concentrations in an animal — a plant consumer and tapeworm host. Environ Sci Pollut Res 25:4190–4196. https://doi.org/10.1007/s11356-017-0787-3 CrossRefGoogle Scholar
- Jin T, Nordberg M, Frech W, Dumont X, Bernard A, Ye T, Kong Q, Wang Z, Li P, Lundström NG, Li Y, Nordberg GF (2002) Cadmium biomonitoring and renal dysfunction among a population environmentally exposed to cadmium from smelting in China (ChinaCad). BioMetals 15:397–410. https://doi.org/10.1023/A:1020229923095 CrossRefGoogle Scholar
- Lee DY, Prasad AS, Hydrick-Adair C, Brewer G, Johnson PE (1993) Homeostasis of zinc in marginal human zinc deficiency: role of absorption and endogenous excretion of zinc. J Lab Clin Med 122:549–556Google Scholar
- Petering HG (1979) Effect of cadmium and lead on copper and zinc metabolism. Trace Element Metab Anim Proc Int Symp 2Google Scholar
- Roohani N, Hurrell R, Kelishadi R, Schulin R (2013) Zinc and its importance for human health: an integrative review. J Res Med Sci 18:144–157Google Scholar
- Sarret G, Willems G, Isaure MP, Marcus MA, Fakra SC, Frérot H, Pairis S, Geoffroy N, Manceau A, Saumitou-Laprade P (2009) Zinc distribution and speciation in Arabidopsis halleri × Arabidopsis lyrata progenies presenting various zinc accumulation capacities. New Phytol 184:581–595. https://doi.org/10.1111/j.1469-8137.2009.02996.x CrossRefGoogle Scholar
- Winiarska-Mieczan A, Kwiecień M (2016) The effect of exposure to Cd and Pb in the form of a drinking water or feed on the accumulation and distribution of these metals in the organs of growing Wistar rats. Biol Trace Elem Res 169:230–236. https://doi.org/10.1007/s12011-015-0414-4 CrossRefGoogle Scholar
- Zheng G, Zhong H, Guo Z, Wu Z, Zhang H, Wang C, Zhou Y, Zuo Z (2014) Levels of heavy metals and trace elements in umbilical cord blood and the tisk of adverse pregnancy outcomes: a population-based study. Biol Trace Elem Res 160:437–444. https://doi.org/10.1007/s12011-014-0057-x CrossRefGoogle Scholar