Molecular isolation and characterization of the kisspeptin system, KISS and GPR54 genes in roach Rutilus rutilus—
The reproduction of vertebrates is regulated by endocrine and neuro-endocrine signaling molecules acting along the brain-pituitary-gonad (BPG) axis. The understanding of the neuroendocrine role played in reproductive function has been recently revolutionized since the KiSS1/GPR54 (KiSS1r) system was discovered in 2003 in human and mice. Kisspeptins, neuropeptides that are encoded by the KiSS genes, have been recognized as essential in the regulation of the gonadotropic axis. They have been shown to play key roles in puberty onset and reproduction by regulating the gonadotropin secretion in mammals while physiological roles in vertebrates are still poorly known. In order to provide new knowledge on basic reproductive physiology in fish as well as new tools to assess impacts of endocrine disrupting compounds (EDCs), the neurotransmitter system, i.e., gene/receptor, KISS/GPR54 might constitute an appropriate biomarker. This study provides new understandings on the neuroendocrine regulation of roach reproduction as well as new molecular tools to be used as biomarkers of endocrine disruption. This work completes the set of biomarkers already validated in this species.
KeywordsBiomarkers Endocrine disruption Fish Neuropeptides Brain pituitary gonad axis
This project was supported by the European program Interreg III and IVA (DIESE). Time allocated to Perrine Geraudie was partially covered by the funding program "Hazardous substances" from the High North Research Centre for Climate and the Environment. Framsenteret, with the project number 9165 M.
- Cachot J, Geffard O, Augagneur S, Lacroix S, Le Menach K, Peluhet L, Couteau J, Denier X, Devier MH, Pottier D, Budzinski H (2006) Evidence of genotoxicity related to high PAH content of sediments in the upper part of the seine estuary (Normandy), France. Aquat Toxicol 79:257–267CrossRefGoogle Scholar
- Geraudie P, Gerbron M, Minier (2017) Endocrine disruption effects in male and intersex roach (Rutilus rutilus, L.) from French rivers: an integrative approach based on subcellular to individual responses. Comp Biochem Physiol B Biochem Mol Biol. https://doi.org/10.1016/j.cbpb.2017.05.006 CrossRefGoogle Scholar
- Mechaly AS, Viñas J, Piferrer F (2011) Gene structure analysis of kisspeptin-2(Kiss2) in the Senegalese sole (Solea senegalensis): characterization of two splice variants of Kiss2, and novel evidence for metabolic regulation of kisspeptin signaling in non-mammalian species. Mol Cell Endocrinol 339:14–24CrossRefGoogle Scholar
- Ohtaki T, Shintani Y, Honda S, Matsumoto H, Hori A, Kanehashi K, Terao Y, Kumano S, Takatsu Y, Masuda Y, Ishibashi Y, Watanabe T, Asada M, Yamada T, Suenaga M, Kitada C, Usuki S, Kurokawa T, Onda H, Nishimura O, Fujino M (2001) Metastasis suppressor gene KiSS-1 encodes peptide ligand of a G-protein coupled receptor. Nature 411(6387):613–617CrossRefGoogle Scholar
- Seminara SB, Messager S, Chatzidaki EE, Thresher RR, Acierno JS Jr, Shagoury JK, Bo-Abbas Y, Kuohung W, Schwinof KM, Hendrick AG, Zahn D, Dixon J, Kaiser UB, Slaugenhaupt SA, Gusella JF, O’Rahilly S, Carlton MB, Crowley WF Jr, Aparicio SA, Colledge WH (2003) The GPR54 gene as a regulator of puberty. N Engl J Med 349:1614–1627CrossRefGoogle Scholar
- Tyler C, Lange A, Paull G, Katsu Y, Iguchi T (2007) The roach (Rutilus rutilus) as a sentinel for assessing endocrine disruption. Environ Sci 14:235–253Google Scholar
- Vacher C, Mananos EL, Breton B, Marmignon MH, Saligaut C (2000) Modulation of pituitary dopamine D1 or D2 receptors and secretion of follicle-stimulating hormone and luteinizing hormone during the annual reproductive cycle of female rainbow trout. J Neuroendocrinol 12:1219–1226CrossRefGoogle Scholar