Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Genotoxicity of water samples from an area of the Pampean region (Argentina) impacted by agricultural and livestock activities

Abstract

The aim of this study was to assess the genotoxic potential of surface waters located in a rural area in the north east of Buenos Aires province (Argentina) using the Allium cepa test. Water samples were collected at four sites located in a drainage channel and two sites on the Burgos stream that receives water from the channel, taking into account the sowing and harvesting months and rainfall periods. Analytical determinations revealed high total concentrations of Cd, Cu, Pb, and Zn (maximum values: 0.030, 0.252, 0.176, and 0.960 mg L−1, respectively), and concentrations of glyphosate and its metabolite aminomethylphosphonic acid (AMPA), with maximum values of 13.6 and 9.75 μg L−1, respectively. Statistically positive correlations were observed between the total metal concentrations and precipitation. No cytotoxicity (mitotic index MI) was observed in A. cepa. However, several water samples showed significant increases in micronucleus (MN) frequencies with respect to the controls. No correlations were observed between MN and the abiotic variables or precipitation. These results showed a state of deterioration in the water quality at the rural area studied in Buenos Aires province, and heavy metal contamination may contribute to the genotoxic activity. A. cepa was shown to be a useful tool for the detection of genotoxicity in water samples from areas with agricultural and livestock activities.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2

References

  1. Alloway BJ (1995) Heavy metals in soils, 2nd edn. Blackie, Glasgow 368 p

  2. Aparicio VC, De Gerónimo E, Marino D, Primost J, Carriquiriborde P, Costa JL (2013) Environmental fate of glyphosate and aminomethylphosphonic acid in surface waters and soil of agricultural basins. Chemosphere 93(9):1866–1873

  3. APHA-AWWA-WPCF (2012) American Public Health Association, American Water Works Association, Water Environment Federation. Standard methods for the examination of water and wastewater. 22nd edn. USA

  4. Arreghini S, de Cabo L, de Iorio AF (2006) Phytoremediation of two types of sediment contaminated with zn by Schoenoplectus americanus. Int J Phytoremediation 8:223–232

  5. Arreghini S, de Cabo L, Seoane R, Tomazin N, Serafini R, de Iorio AF (2007) A methodological approach to water quality assessment in an ungauged basin (Buenos Aires, Argentina). GeoJournal 70:281–288

  6. Arreghini S, de Cabo L, Serafini R, de Iorio AF (2017) Effect of the combined addition of Zn and Pb on partitioning in sediments and their accumulation by the emergent macrophyte Schoenoplectus californicus. Environ Sci Pollut Res 24(9):8098–8107

  7. Avigliano L, Fassiano AV, Medesani DA, Ríos de Molina MC, Rodríguez EM (2014) Effects of glyphosate on growth rate, metabolic rate and energy reserves of early juvenile crayfish, Cherax quadricarinatus M. Bull Environ Contam Toxicol 92(6):631–635

  8. Barbosa JS, Cabral TM, Ferreira DN, Agnez-Lima LF, Batistuzzo de Medeirosa SR (2010) Genotoxicity assessment in aquatic environment impacted by the presence of heavy metals. Ecotoxicol Environ Saf 73:320–325

  9. Basílico G, Magdaleno A, Paz M, Moretton J, Faggi A, de Cabo L (2017) Sewage pollution: genotoxicity assessment and phytoremediation of nutrients excess with Hydrocotyle ranunculoides. Environ Monit Assess 189:182

  10. Battaglin WA, Furlong ET, Burkhardt MR, Peter CJ (2000) Occurrence of sulfonylurea, sulfonamide, imidazolinone, and other herbicides in rivers, reservoirs and ground water in the midwestern United States (1998). Sci Total Environ 248:123–133

  11. Borggaard OK, Gimsing AL (2008) Fate of glyphosate in soil and the possibility of leaching to ground and surface waters: a review. Pest Manag Sci 64:441–456

  12. Cappi da Costa T, Tagliari de Brito KC, Vaz Rocha JA, Leal KA, Kolowski Rodrigues ML, Gomes Minella JP, Matsumoto ST, Vargas VMF (2012) Runoff of genotoxic compounds in river basin sediment under the influence of contaminated soils. Ecotoxicol Environ Saf 75(1):63–72

  13. Chagas CI, Kraemer FB, Santanatoglia OJ, Paz M, Moretton J (2014) Biological water contamination in some cattle production fields of Argentina subjected to runoff and erosion. Span J Agric Res 12(4):1008–1017

  14. de Castilhos Ghisi N, de Oliveira EC, Prioli AC (2016) Does exposure to glyphosate lead to an increase in the micronuclei frequency? A systematic and meta-analytic review. Chemosphere 145:42–54

  15. Djukić A, Lekić B, Rajaković-Ognjanović V, Veljović D, Vulić T, Djolić M, Naunovic Z, Despotović J, Prodanović D (2016) Further insight into the mechanism of heavy metals partitioning in stormwater runoff. J Environ Manag 168:104–110

  16. Fatima RA, Ahmad M (2006) Genotoxicity of industrial wastewaters obtained from two different pollution sources in northern India: a comparison of three bioassays. Mutat Res 609:81–91

  17. Fedorova AI, Kalaev VN, Prosvirina YG, Goryainova SA (2007) Mutagenic activity of heavy metals in soils of wayside slopes. Eurasian Soil Sci 40(8):893–899

  18. Fergusson J (1990) The heavy elements. Chemistry, environmetal impact and health effects. Pergamon Press, Oxford, pp 175–182

  19. Fiskesjǒ G (1985) The Allium test as a standard in environmental monitoring. Hereditas 102:99–112

  20. Florea AM, Busselberg D (2006) Occurrence, use and potential toxic effects of metals and metal compounds. Biometals 19:419–427

  21. Goscinny S, Unterluggauer H, Aldrian J, Hanot V, Masselter S (2012) Determination of glyphosate and its metabolite AMPA (aminomethylphosphonic acid) in cereals after derivatization by isotope dilution and UPLC-MS/MS. Food Anal Methods 5:1177–1185

  22. Grant WF (1999) Higher plant assays for the detection of chromosomal aberrations and gene mutation—a brief historical background on their use for screening and monitoring environmental chemicals. Mutat Res 426:107–112

  23. Hoshina MM, Marin-Morales MA (2009) Micronucleus and chromosome aberrations induced in onion (Allium cepa) by a petroleum refinery effluent and by river water that receives this effluent. Ecotoxicol Environ Saf 72:2090–2095

  24. Khan SJ, Roser DJ, Davies CM, Peters GM, Stuetz RM, Tucker R, Ashbolt NJ (2008) Chemical contaminants in feedlot wastes: concentrations, effects and attenuation. Environ Int 34:839–859

  25. Kier LD, Kirkland DJ (2013) Review of genotoxicity studies of glyphosate and glyphosate-based formulations. Crit Rev Toxicol 43(4):283–315

  26. Kim R-Y, Yoon J-K, Kim T-S, Yang JE, Owens G, Kim K-R (2015) Bioavailability of heavy metals in soils: definitions and practical implementation—a critical review. Environ Geochem Health 37:1041–1061

  27. Kraemer FB, Chagas CI, Irurtia C, Garibaldi LA (2011) Bacterial retention in three soils of the Rolling Pampa, Argentina, under simulated rainfall. J Soil Sci Environ Manage 2(11):341–353

  28. Krüger M, Schledorn P, Schrödl W, Hoppe H-W, Lutz W, Shehata AA (2014) Detection of glyphosate residues in animals and humans. J Environ Anal Toxicol 4:210. https://doi.org/10.4172/2161-0525.1000210

  29. Lavado RS, Porcelli CA, Alvarez R (2001) Nutrient and heavy metal concentration and distribution in corn, soybean and wheat as affected by different tillage systems in the Argentine Pampas. Soil Tillage Res 62(1–2):55–60

  30. Leme DM, Marin-Morales MA (2009) Allium cepa test in environmental monitoring: a review on its application. Mutat Res 682:71–81

  31. Mackereth F, Heron J, Talling J (1989) Water analysis: some revised methods for limnologists, 2nd edn. Freshwater Biological Association, Cumbria Scientific publication No. 36

  32. Magdaleno A, Mendelson A, Fabrizio de Iorio A, Rendina A, Moretton J (2008) Genotoxicity of leachates from highly polluted lowland river sediments destined for disposal in landfill. Waste Manag 28:2134–2139

  33. Mañas F, Peralta L, Raviolo J, Ovando HG, Weyers A, Ugnia L, Cid MG, Larripa I, Gorla N (2009) Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests. Environ Toxicol Pharmacol 28:37–41

  34. Manassero M, Camilión C, Ronco A (2004) Análisis textural de sedimentos fluviales distales de arroyos de la pampa ondulada, Provincia de Buenos Aires, Argentina. Asociación Argentina de Sedimentología 11(2):57–68

  35. Manz M, Weissflog L, Kühne R, Schüürmann G (1999) Ecotoxicological hazard and risk assessment of heavy metal contents in agricultural soils of central Germany. Ecotoxicol Environ Saf 42:191–201

  36. Marino D, Ronco A (2005) Cypermethrin and chlorpyrifos concentration levels in surface water bodies of the Pampa Ondulada, Argentina. Bull Environ Contam Toxicol 75:820–826

  37. Matsumoto ST, Mantovani MS, Malagutti MIA, Dias AL, Fonseca IC, Marin-Morales MA (2006) Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips. Genet Mol 29:148–158

  38. Mendoza RE, García IV, de Cabo L, Weigandt CF, Fabrizio de Iorio A (2015) The interaction of heavy metals and nutrients present in soil and native plants with arbuscular mycorrhizae on the riverside in the Matanza-Riachuelo River Basin (Argentina). Sci Total Environ 505:555–564

  39. Nedelkoska TV, Low GK-C (2004) High-performance liquid chromatographic determination of glyphosate in water and plant material after pre-column derivatisation with 9-fluorenylmethyl chloroformate. Anal Chim Acta 511:145–153

  40. Peluso M, Munnia A, Bolognesi C, Parodi S (1998) 32P-Postlabeling detection of DNA adducts in mice treated with the herbicide roundup. Environ Mol Mutagen 31:55–59

  41. Peruzzo PJ, Porta AA, Ronco AE (2008) Levels of glyphosate in surface waters, sediments and soils associated with direct sowing soybean cultivation in north pampasic region of Argentina. Environ Pollut 156:61–66

  42. Pignata ML, Gudiño GL, Wannaza ED, Plá RR, González CM, Carreras HA, Orellanac L (2002) Atmospheric quality and distribution of heavy metals in Argentina employing Tillandsia capillaris as a biomonitor. Environ Pollut 120:59–68

  43. Rank J, Nielsen MH (1998) Genotoxicity testing of wastewater sludge using the Allium cepa anaphase-telophase chromosome aberration assay. Mutat Res 418:113–119

  44. Rank J, Jensen AG, Skov B, Pedersen LH, Jensen K (1993) Genotoxicity testing of the herbicide roundup and its active ingredient glyphosate isopropylamine using the mouse bone marrow micronucleus test, Salmonella mutagenicity test, and Allium anaphase-telophase test. Mutat Res 300:29–36

  45. Romero DM, Ríos de Molina MC, Juárez AB (2011) Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri. Ecotoxicol Environ Safety 74:741–747

  46. Soracco CG, Lozano LA, Villarreal R, Melani E, Sarli GO (2018) Temporal variation of soil physical quality under conventional and no-till systems. Rev Bras Cienc Solo 42:e0170408

  47. Sprankle P, Meggitt WF, Penner D (1975) Adsorption, mobility and microbial degradation of glyphosate in the soil. Weed Sci 23:229–234

  48. Strickland J, Parsons T (1972) A practical handbook of seawater analysis, 2nd edn. Fisheries Research Board of Canada, Ottawa Bulletin No. 167

  49. U.S. Environmental Protection Agency (2009) National recommended water quality crieteria 4304T, http://www.epa.gov/ost/criteria/wqctable

  50. Uusi-Kämppä J, Jauhiainen L, Huuskonen A (2007) Phosphorus and nitrogen losses to surface waters from a forested feedlot for bulls in Finland. Soil Use Manag 23(1):82–91

  51. Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M (2006) Free radicals, metals and antioxidants in oxidative stress induced cancer. Chem Biol Interact 160:1–40

  52. Vargas VMF, Migliavacca SB, de Melo AC, Horn RC, Guidobono RR, Ferreira ICFS, Pestana MHD (2001) Genotoxicity assessment in aquatic environments under the influence of heavy metals and organic contaminants. Mutat Res 490:141–158

  53. Zhang F, Li Y, Yang M, Li W (2012) Content of heavy metals in animal feeds and manures from farms of different scales in Northeast China. Int J Environ Res Public Health 9:2658–2668

Download references

Acknowledgments

The authors are grateful to Mrs. Amalia Gonzalez for the artwork of the Burgos stream basin, and Mr. Ricardo J. Piccolo for kindly providing the Allium cepa seeds for performing the genotoxicity assay.

Funding

This study was supported financially by the Buenos Aires University, Argentina, under Projects UBACyT No. 20020130100601BA and 20020150200116BA.

Author information

Correspondence to Anahí Magdaleno.

Additional information

Responsible editor: Markus Hecker

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Bollani, S., de Cabo, L., Chagas, C. et al. Genotoxicity of water samples from an area of the Pampean region (Argentina) impacted by agricultural and livestock activities. Environ Sci Pollut Res 26, 27631–27639 (2019). https://doi.org/10.1007/s11356-018-3263-9

Download citation

Keywords

  • Allium cepa
  • Chromosome aberration
  • Micronucleous
  • Aquatic toxicology
  • Genotoxicity
  • Heavy metals