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Environmental Science and Pollution Research

, Volume 25, Issue 31, pp 31427–31438 | Cite as

Physicochemical properties of acid- and pepsin-soluble collagens from the cartilage of Siberian sturgeon

  • Qian-Bin Luo
  • Chang-Feng Chi
  • Fan Yang
  • Yu-Qin Zhao
  • Bin Wang
Research Article

Abstract

To look for the collagen alternatives of mammalian cartilages from aquatics and their by-products, acid-soluble collagen (ASC-SC) and pepsin-soluble collagen (PSC-SC) were extracted from cartilages of Siberian sturgeon (Acipenser baerii) with yields of 27.13 ± 1.15 and 14.69 ± 0.85% on dry weight basis. ASC-SC and PSC-SC had glycine as the major amino acid with the contents of 326.8 and 327.5 residues 1000 residues−1, and their contents of proline and hydroxyproline were 205.9 and 208.0 residues 1000 residues−1. ASC-SC and PSC-SC comprised type I collagen ([α1(I)]2α2(I)) and type II collagen ([α1(II)]3) on the literatures and results of amino acid composition, SDS-PAGE pattern, UV, and FTIR spectra. Meanwhile, FTIR spectra data indicated that there were more hydrogen bonds in ASC-SC and more intermolecular crosslinks in PSC-SC. The maximum transition temperature (Tmax) of the ASC (28.3 °C) and PSC (30.5 °C) was lower than those of collagens from mammalian cartilages (> 37 °C). ASC-SC and PSC-SC showed high solubility in the acidic pH ranges and the solubility decreased in the presence of NaCl at concentrations above 3%. Zeta potential studies indicated that both ASC-SC and PSC-SC exhibited a net zero charge at pH 6.30 and 6.32. SEM results indicated that ASC-SC and PSC-SC presented irregular dense sheet-like film linked by random-coiled filaments. Therefore, collagens from Siberian sturgeon cartilages might be the suitable alternatives of the collagens of mammal cartilages as functional ingredient to treat some diseases.

Keywords

Siberian sturgeon (Acipenser baeriiCartilage Acid-soluble collagen (ASC) Pepsin-soluble collagen (PSC) Antioxidant activity 

Notes

Funding information

This work was funded by the National Natural Science Foundation of China (NSFC) (No. 81673349), Science and Technology Program of Zhoushan (No. 2016C41016), Natural Science Foundation of Zhejiang Province, China (No. LY15C190010), and Open Foundation from Marine Sciences in the Most Important Subjects of Zhejiang (No. 20160116).

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and TechnologyZhejiang Ocean UniversityZhoushanChina
  2. 2.Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and PharmacyZhejiang Ocean UniversityZhoushanChina

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