Micronucleus frequency is correlated with antioxidant enzyme levels in workers occupationally exposed to pesticides
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Oxidative stress can cause DNA damage leading to nuclear anomalies such as micronuclei (MN). Antioxidant enzymes involved in protection against intracellular oxidative stress include glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), and catalase (CAT). Pesticide exposure induces oxidative stress and alters antioxidant defense mechanisms, including detoxification and scavenger enzymes. The aim of this study was to evaluate MN frequency in workers occupationally exposed to pesticides and their relationship with antioxidant enzyme activities. A cross-sectional study was conducted in 201 individuals, some of whom were dedicated to the spraying of pesticides. The cytokinesis-block micronucleus (CBMN) assay was conducted, and the activities of GPx, GR, SOD, and CAT were determined. The geometric mean (GM) of MN was 5.4 (1–26 MN). The GM for the antioxidant enzymes was 198.68 U/mL for GPx, 38.96 U/g Hb for GR, 94.78 U/mL for SOD, and 69.77 U/g Hb for CAT. There was a lower MN frequency in males than that in females, and a higher nuclear index. In addition, age affected MN frequency. There was a negative correlation between MN frequency and GPx activity, but a positive one between MN frequency and GR activity. These findings suggest the involvement of GPx in MN frequency.
KeywordsMicronuclei Antioxidant enzymes Pesticides exposure
The authors would like to thank Rigoberto Zepeda Arce for his technical assistance; Cyndia Azucena González Arias and Norma Elena Pérez Herrera for their critical review of the manuscript; to Leticia Yañez Estrada and the team of the Gender, Health and Environmental Laboratory of the Medicine Faculty, San Luis Potosi Autonomous University, for their help in DAP determination. The authors also would like to thank all study participants.
This study was supported by CONACyT-SALUD-Mexico-233803.
Compliance with ethical standards
All participants signed an informed consent at the beginning of the study. This study was approved by the Bioethics Commission of Nayarit State, Mexico (CEBN/0112017).
Conflict of interest
The authors declare that there are no conflicts of interest.
- Abdollahi M, Ranjbar A, Shadnia S, Nikfar S, Rezale A (2004) Pesticides and oxidative stress: a review. Med Sci Monit 10:141–147Google Scholar
- Barale R, Chelotti L, DelRy S, Andreassi MG, Ballardin M, Bulleri M, He J, Baldacci S, Di Pede F, Gemignani F, Landi S (1998) Sister chromatid exchange and micronucleus frequency in human lymphocytes of 1,650 subjects in an Italian population: II. Contribution of sex, age, and lifestyle. Environ Mol Mutagen 31:228–242CrossRefGoogle Scholar
- Benitez-Trinidad AB, Herrera-Moreno JF, Vázquez-Estrada G, Verdín-Betancourt FA, Sordo M, Ostrosky-Wegman P, Bernal-Hernández YY, Medina-Díaz IM, Barrón-Vivanco BS, Robledo-Marenco MI, Salazar AM, Rojas-García AE (2015) Cytostatic and genotoxic effect of themeohos in human lymphocytes and HepG2 cells. Toxicol in Vitro 29:779–786CrossRefGoogle Scholar
- Bolognesi C, Abbondandolo A, Barale R, Casalone R, Dalpra L, De Ferri M, Degrassi F, Forni A, Lamberti L, Lando C, Migliore L, Padovani P, Pasquini R, Puntoni R, Sbrana I, Stella M, Bonassi S (1997) Age-related increase of baseline frequencies of sister chromatid exchanges, chromosome aberrations, and micronuclei in human lymphocytes. Cancer Epidemiol Biomark Prev 6:249–256Google Scholar
- Bonassi S, Znaor A, Ceppi CL, Chang WP, Holland N, Kirsch-Volders M, Zeiger E, Ban S, Barale R, Bigatti MP, Bolognesi C, Cebulska-Wasilewska A, Fabianova E, Fucic A, Hagmar L, Joksic G, Martelli A, Migliore L, Mirkova E, Scarfi MR, Zijno A, Norppa H, Fenech M (2007) An increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of cancer in humans. Carcinogenesis 28:625–631CrossRefGoogle Scholar
- Cervantes Ríos E, Ortiz Muñiz R, Konigsberg Fainstein M, Graniel Guerrero J, Rodríguez Cruz L (2018) Assessment of micronucleus and oxidative stress in peripheral blood from malnourished children. Nutr Hosp 35:519–526Google Scholar
- Giergiel M, Lopucki M, Stachowicz N, Kankofer M (2012) The influence of age and gender on antioxidant enzyme activities in humans and laboratory animals. Aging Clin Exp Res 24:561–569Google Scholar
- Khayat CB, Costa EO, Gonçalves MW, da Cruz e Cunha DM, da Cruz AS, de Araújo Melo CO, Bastos RP, da Cruz AD, de Melo e Silva D (2013) Assessment of DNA damage in Brazilian workers occupationally exposed to pesticides: a study from Central Brazil. Environ Sci Pollut Res 20:7334–7340CrossRefGoogle Scholar
- Lee C-H, Kamijima M, Kim H, Shibata E, Ueyama J, Suzuki T, Takagi K, Saito I, Gotoh M, Hibi H, Naito H, Nakajima T (2006) 8-Hydroxydeoxyguanosine levels in human leukocyte and urine according to exposure to organophosphorus pesticides and paraoxonase 1genotype. Int Arch Occup Environ Health 80:217–227CrossRefGoogle Scholar
- Matarrese P, Colasanti T, Ascione B, Margutti P, Franconi F, Alessandri C, Conti F, Riccieri V, Rosano G, Ortona E, Malorni W (2011) Gender disparity in susceptibility to oxidative stress and apoptosis induced by autoantibodies specific to RLIP76 in vascular cells. Antioxid Redox Signal 15:2825–2836CrossRefGoogle Scholar
- Pajović SB, Saičić ZS (2008) Modulation of antioxidant enzyme activities by sexual steroid hormones. Physiol Res 57:801–811Google Scholar
- Ramírez-Jiménez R, Mejía-Saucedo R, Calderón-Hernández J, Montero-Montoya R, Yáñez-Estrada L (2014) Concentraciones urinarias de metabolitos de plaguicidas organofosforados en niños y adolescentes de una zona agrícola de México. Revista Iberoamericana de Ciencias 1:87–94Google Scholar
- Richard F, Aurias A, Couturier J, Dutrillaux AM, Flury-Herard A, Gerbautl-Seureau M, Hoffschir F, Lamoliatte E, Lefrancois D, Lombard M, Muleris M, Prieur M, Ricoul M, Sabatier L, Viegas-Péquignot E, Volobouev V, Dutrillaux B (1993) Aneuploidy in human lymphocytes: an extensive study of eight individuals of various ages. Mutat Res 295:71–80CrossRefGoogle Scholar
- WHO (2006) World Health Organization. BMI classification. Global database on body mass index. http://apps.who.int/bmi/index.jsp?introPage=intro_3.html
- WHO (2009) World Health Organization. The WHO recommended classification of pesticides by hazard and guidelines to classification. http://apps.who.int/iris/bitstream/10665/44271/1/9789241547963_eng.pdf
- Zepeda-Arce R, Rojas-García AE, Benitez-Trinidad A, Herrera-Moreno JF, Medina-Díaz IM, Barrón-Vivanco BS, Pier Villegas G, Hernández-Ochoa I, Sólis Heredia MJ, Bernal-Hernández YY (2017) Oxidative stress and genetic damage among workers exposed primarily to organophosphate and pyrethroid pesticides. Environ Toxicol 32:1754–1764CrossRefGoogle Scholar