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Environmental Science and Pollution Research

, Volume 25, Issue 26, pp 26341–26350 | Cite as

Assessment of hepato-renal damage and genotoxicity induced by long-term exposure to five permitted food additives in rats

  • Khaled Abo-EL-Sooud
  • Mohamed M. Hashem
  • Yahia A. Badr
  • Mona M. E. Eleiwa
  • Ali Q. Gab-Allaha
  • Yasmina M. Abd-ElhakimEmail author
  • Ahmed Bahy-EL-Dien
Research Article
  • 176 Downloads

Abstract

The present study assessed the long-term daily administration of benzoic acid (BA), potassium sorbate (PS), chlorophyll (CPL), tartrazine (TAZ), and butylated hydroxyanisole (BHA) on hepato-renal changes and DNA damage in rats. Animals were orally administered with the 10 times of the acceptable daily intake (ADI) from each tested substance daily for 60 consecutive days. Blood, liver, and kidney samples were collected to evaluate hematological, biochemical, histopathological, and genotoxic alterations. The extent of liver and kidney damage was evaluated by comet assay and histopathologically. Significant reduction of leukocyte numbers and lymphocytes % in CPL- and TAZ-treated rats. However, significant increases in platelet count in all treated groups after 60 days were detected. The levels of serum transaminases enzymes (ALT, AST), alkaline phosphatase (ALP), and creatinine were significantly increased in all treatments except with BHA group, but no substantial differences were found in urea after 60 days. Aside from BHA, results of DNA damage revealed significant increases in tailed nuclei, tail moment, DNA% in the tail, and tail length in liver and kidney at different degrees. Moreover, the histopathological figures of liver and kidneys affirmed destructive and degenerative changes. The study indicates that most of the tested food additives may provoke genotoxicity and hepato-nephropathy, which could be serious for human health. Therefore, it is necessary to be informed about the hazardous effects of food additives and more attention should be focused towards using natural substitutes.

Keywords

Food additives Benzoic acid Potassium sorbate Chlorophyll Tartrazine Butylated hydroxyanisole 

Notes

Acknowledgments

This work was funded by Cairo University, Project entitled “Assessment of Residual patterns and Genotoxicity of some food additives using LMRS (laser molecular Raman spectroscopy).”

References

  1. Aboel-Zahab H, El-Khyat Z, Sidhom G, Awadallah R, Abdel-Al W, Mahdy K (1997) Physiological effects of some synthetic food colouring additives on rats. Boll Chim Farm 136:615–627Google Scholar
  2. Abd-Elhakim YM, Hashem MM, Anwar A, El-Metwally AE, Abo-El-Sooud K, Moustafa GG, Mouneir SM, Ali HA (2018) Effects of the food additives sodium acid pyrophosphate, sodium acetate, and citric acid on hemato-immunological pathological biomarkers in rats: relation to PPAR-α, PPAR-γ and TNFα signaling pathway Environmental Toxicology and Pharmacology  https://doi.org/10.1016/j.etap.2018.07.002
  3. Al-Seeni MN, El Rabey HA, Al-Hamed AM, Zamazami MA, (2018) Nigella sativa oil protects against tartrazine toxicity in male rats. Toxicol Rep 5:146–155Google Scholar
  4. Alsuhaibani AM, ALkehayez NM, Alshawi Amal H, Al-Faris NA (2017) Effects of chlorophyll on body functioning and blood glucose levels. Asian J Clin Nutr 9:64–70CrossRefGoogle Scholar
  5. Amin K, Hameid HA II, Elsttar AA (2010) Effect of food azo dyes tartrazine and carmoisine on biochemical parameters related to renal, hepatic function and oxidative stress biomarkers in young male rats. Food Chem Toxicol 48:2994–2999CrossRefGoogle Scholar
  6. Ashour AA, Abdelaziz I (2009) Role of fast green on the blood of rats and the therapeutic action of vitamins C or E. Int J Integr Biol 6:6–11Google Scholar
  7. Bain B, Bates I, Laffan MA, Lewis SM (2012) Dacie and Lewis practical haematology, 11th edn. Churchill Livingstone, Elsevier, PhiladelphiaGoogle Scholar
  8. Bautista ARPL, Moreira E, Batista MS, Miranda M, Gomes I (2004) Subacute toxicity assessment of annatto in rat. Food Chem Toxicol 42:625–629CrossRefGoogle Scholar
  9. Bloom AS, Russell LJ, Weisskopf B, Blackerby JL (1988) Methylphenidate-induced delusional disorder in a child with attention deficit disorder with hyperactivity. J Am Acad Child Adolesc Psychiatry 27:88–89CrossRefGoogle Scholar
  10. Boindogurong J, Higaki T, Egashira Y, Sanada H (2005) Protective effect of butylated hydroxyanisole and butylated hydroxytoluene against acetaminophen-induced hepatotoxicity in rats. J Oleo Sci 54:153–159CrossRefGoogle Scholar
  11. Borzelleca J, Hallagan J (1988) Chronic toxicity/carcinogenicity studies of FD & C Yellow No. 5 (tartrazine) in rats. Food Chem Toxicol 26:179–187CrossRefGoogle Scholar
  12. Branen A, Davidson PM, Salminen S, Thorngate J (2001) Food additives. Taylor & Francis, New YorkGoogle Scholar
  13. De Boeck M, Touil N, De Visscher G, Vande PA, Kirsch-Volders M (2000) Validation and implementation of an internal standard in comet assay analysis. Mutat Res Genet Toxicol Environ Mutagen 469:181–197CrossRefGoogle Scholar
  14. Devlin J, David T (1992) Tartrazine in atopic eczema. Arch Dis Child 67:709–711CrossRefGoogle Scholar
  15. Duez P, Dehon G, Kumps A, Dubois J (2003) Statistics of the comet assay: a key to discriminate between genotoxic effects. Mutagenesis 18:159–166CrossRefGoogle Scholar
  16. El-Wahab HMFA, Moram GSE-D (2013) Toxic effects of some synthetic food colorants and/or flavor additives on male rats. Toxicol Ind Health 29:224–232CrossRefGoogle Scholar
  17. Frankel EN (1998) Free radical oxidation. In: Frankel EN (ed) Lipid oxidation. The Oily Press, Scotland, pp 13–22Google Scholar
  18. Hashem MM, Atta AH, Arbid MS, Nada SA, Asaad GF (2010) Immunological studies on amaranth, sunset yellow and curcumin as food colouring agents in albino rats. Food Chem Toxicol 48:1581–1586CrossRefGoogle Scholar
  19. Hassan RI, Raheem GSA (2016) Effect of feeding benzoic acid on performance of broiler chickens journal of advanced. Vet Res 6:118–122Google Scholar
  20. Himri I et al (2011) A 90-day oral toxicity study of tartrazine, a synthetic food dye, in wistar rats. Group 300:00Google Scholar
  21. Hood W (2012) A–Z of clinical chemistry: a guide for the trainee. Springer Science & Business MediaGoogle Scholar
  22. Kahl R, Kappus H (1993) Toxicology of the synthetic antioxidants BHA and BHT in comparison with the natural antioxidant vitamin E. Z Lebensm Unters Forsch 196:329–338CrossRefGoogle Scholar
  23. King CM, Land SJ, Jones RF, Debiec-Rychter M, Lee M-S, Wang CY (1997) Role of acetyltransferases in the metabolism and carcinogenicity of aromatic amines. Mutat Res Fundam Mol Mech Mutagen 376:123–128CrossRefGoogle Scholar
  24. Kitamura Y, Nishikawa A, Furukawa F, Nakamura H, Okazaki K, Umemura T, Imazawa T, Hirose M (2003) A subchronic toxicity study of shea nut color in Wistar rats. Food Chem Toxicol 41:1537–1542CrossRefGoogle Scholar
  25. Maki T, Suzuki Y (1985) Benzoic acid and derivatives. In: Ullmann’s encyclopedia of industrial chemistry. Vol. A3. Weinheim, Published by VCH Verlagsgesell schaft, Germany, pp 555–568Google Scholar
  26. Mamur S, Yüzbaşıoğlu D, Ünal F, Yılmaz S (2010) Does potassium sorbate induce genotoxic or mutagenic effects in lymphocytes? Toxicol in Vitro 24:790–794CrossRefGoogle Scholar
  27. Mpountoukas P, Pantazaki A, Kostareli E, Christodoulou P, Kareli D, Poliliou S, Mourelatos C, Lambropoulou V, Lialiaris T (2010) Cytogenetic evaluation and DNA interaction studies of the food colorants amaranth, erythrosine and tartrazine. Food Chem Toxicol 48:2934–2944CrossRefGoogle Scholar
  28. Myllyperkiö M, Koski T, Vilpo L, Vilpo J (1999) Gama-irradiation-induced DNA single-and double-strand breaks and their repair in chronic lymphocytic leukemia cells of variable radiosensitivity. Hematol Cell Ther 41:95–103CrossRefGoogle Scholar
  29. Okubo T, Yokoyama Y, Kano K, Kano I (2004) Molecular mechanism of cell death induced by the antioxidant tert-butylhydroxyanisole in human monocytic leukemia U937 cells. Biol Pharm Bull 27:295–302CrossRefGoogle Scholar
  30. Østergaard G, Knudsen I (1998) The applicability of the ADI (acceptable daily intake) for food additives to infants and children. Food Addit Contam 15:63–74CrossRefGoogle Scholar
  31. Paget GE, Barnes GM (1964) Evaluation of drug activities, vol 1. Academic Press, LondonGoogle Scholar
  32. Pandey H, Kumar V, Roy B (2014) Assessment of genotoxicity of some common food preservatives using Allium cepa L. as a test plant. Toxicol Rep 1:300–308CrossRefGoogle Scholar
  33. Pressinger R (1997) Environmental circumstances that can damage the developing brain, graduate student research project conducted at the University of South Florida. J Pediatr 92:64–67Google Scholar
  34. Richard RT, N.J Totowa (1995) The history of food preservation. Technology in Food Production, 152Google Scholar
  35. Rus V, Gherman C, Miclăuş V, Mihalca A, Nadăş G (2010) Comparative toxicity of food dyes on liver and kidney in guinea pigs: a histopathological study. Ann RSCB 15:161–165Google Scholar
  36. Sasaki YF, Kawaguchi S, Kamaya A, Ohshita M, Kabasawa K, Iwama K, Taniguchi K, Tsuda S (2002) The comet assay with 8 mouse organs: results with 39 currently used food additives. Mutat Res Genet Toxicol Environ Mutagen 519:103–119CrossRefGoogle Scholar
  37. Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175:184–191CrossRefGoogle Scholar
  38. Sugihara N, Tsuruta Y, Furuno K (1998) Effect of potassium sorbate on cellular GSH level and lipid peroxidation in cultured rat hepatocytes. Biol Pharm Bull 21:524–526CrossRefGoogle Scholar
  39. Sulai NH, Tefferi A (2012) Why does my patient have thrombocytosis? Hematol Oncol Clin 26:285–301CrossRefGoogle Scholar
  40. Timbrell JA, Marrs TC (2009) Biotransformation of xenobiotics. General, Applied and Systems ToxicologyGoogle Scholar
  41. Yang X, Schnackenberg LK, Shi Q, Salminen WF (2014) Chapter 13 – Hepatic toxicity biomarkers. In: Gupta RC (ed) Biomarkers in Toxicology. Academic Press, Boston, pp 241–259  https://doi.org/10.1016/B978-0-12-404630-6.00013-0
  42. Yılmaz S, Unal F, Aksoy H, Yuzbasıoglu D, Celik M (2008) Cytogenetic effects of citric acid and benzoic acid on allium chromosomes. Fresen Environ Bull 17:1029–1037Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Pharmacology, Faculty of Veterinary MedicineCairo UniversityGizaEgypt
  2. 2.Department of Laser Sciences and Interactions, National Institute of Laser Enhanced SciencesCairo UniversityGizaEgypt
  3. 3.Department of Botany, Faculty of ScienceCairo UniversityGizaEgypt
  4. 4.The Holding Company for Biological Products & Vaccines VACSERAGizaEgypt
  5. 5.Department of Forensic Medicine and Toxicology, Faculty of Veterinary MedicineZagazig UniversityZagazigEgypt

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