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Environmental Science and Pollution Research

, Volume 25, Issue 26, pp 26539–26549 | Cite as

Enhancing bacterial transport with saponins in saturated porous media for the bioaugmentation of groundwater: visual investigation and surface interactions

  • Yongsheng Zhao
  • Dan Qu
  • Rui Zhou
  • Xinru Yang
  • Wenbo Kong
  • Hejun Ren
Research Article
  • 49 Downloads

Abstract

The success of bioaugmentation processes for the remediation of groundwater contamination relies on effective transport of the injected microorganisms in a subsurface environment. Biosurfactants potentially affect bacterial attachment and transport behavior in porous media. Although saponins as biosurfactants are abundant in nature, their influence on bacterial transport in groundwater systems remains unknown. In this research, tank visual-transport experiments, breakthrough curve monitoring, and surface property measurement were performed to evaluate the effects of saponins on the transport of Pseudomonas migulae AN-1 cells, which were used as a model bacterium in saturated sand. Results show that the 0.1% saponins could effectively facilitated the AN-1 secondary transport and the addition of saponins decreased the hydrophobicity of AN-1 and sand. The role of the promotion of saponins was more dominant than that of the inhibition of ions on AN-1 transport in a saturated porous medium when ions and saponins coexisted. The interactions between AN-1 and sand grains with saponins and ions were explained in accordance with the Derjaguin–Landau–Verwey–Overbeek theory.

Keywords

Saponins Bacterial transport Bioaugmentation Groundwater remediation DLVO 

Notes

Acknowledgements

This study was funded by the National Natural Science Foundation of China (Grant No. 41530636).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

11356_2018_2477_MOESM1_ESM.pdf (262 kb)
ESM 1 (PDF 261 kb)

References

  1. Atlas RM, Hazen TC (2011) Oil biodegradation and bioremediation: a tale of the two worst spills in US history. Environ Sci Technol 45(16):6709–6715CrossRefGoogle Scholar
  2. Brown DG, JAFFE P (2001) Effects of nonionic surfactants on bacterial transport through porous media. Environ Sci Technol 35:3877–3883CrossRefGoogle Scholar
  3. Chen G, Strevett KA (2001) Impact of surface thermodynamics on bacterial transport. Environ Microbiol 3(4):237–245CrossRefGoogle Scholar
  4. Chen G, Zhu H (2005) Bacterial adhesion to silica sand as related to Gibbs energy variations. Colloids Surf. B. Biointerfaces 44(1):41–48CrossRefGoogle Scholar
  5. Chen G, Qiao M, Zhang H, Zhu H (2004) Bacterial desorption in water-saturated porous media in the presence of rhamnolipid biosurfactant. Res Microbiol 155(8):655–661CrossRefGoogle Scholar
  6. Chen WJ, Hsiao LC, Chen KK-Y (2008) Metal desorption from copper(II)/nickel(II)-spiked kaolin as a soil component using plant-derived saponin biosurfactant. Process Biochem 43(5):488–498CrossRefGoogle Scholar
  7. Choi YJ, Kim YJ, Nam K (2009) Enhancement of aerobic biodegradation in an oxygen-limiting environment using a saponin-based microbubble suspension. Environ Pollut 157(8–9):2197–2202CrossRefGoogle Scholar
  8. Da Silva ML, Alvarez PJ (2004) Enhanced anaerobic biodegradation of benzene-toluene-ethylbenzene-xylene-ethanol mixtures in bioaugmented aquifer columns. Appl Environ Microbiol 70(8):4720–4726CrossRefGoogle Scholar
  9. Deflaun MF, Tanzer AS, Mcateer AL, Marshall B, Levy S (1990) Development of an adhesion assay and characterization of an adhesion-deficient mutant of Pseudomonas fluorescens. Appl Environ Microb 56:112–119Google Scholar
  10. Deflaun MF, Oppenheimer SR, Streger S, Condee CW, Fletcher M (1999) Alterations in adhesion, transport, and membrane characteristics in an adhesion-deficient pseudomonad. Appl Environ Microbiol 65(2):759–765Google Scholar
  11. Desai JD, Banat IM (1997) Microbial production of surfactants and their commercial potential. Microbiol Mol Biol Rev 61:47–64Google Scholar
  12. Fan W, Jiang X, Lu Y, Huo M, Lin S, Geng Z (2015) Effects of surfactants on graphene oxide nanoparticles transport in saturated porous media. J Environ Sci 35:12–19CrossRefGoogle Scholar
  13. Graziano G (2010) Hydrophobic interaction of two large plates: an analysis of salting-in/salting-out effects. Chem Phys Lett 491(1):54–58CrossRefGoogle Scholar
  14. Harvey RW, Metge DW, Mohanram A, Gao X, Chorover J (2011) Differential effects of dissolved organic carbon upon re-entrainment and surface properties of groundwater bacteria and bacteria-sized microspheres during transport through a contaminated sandy aquifer. Environ Sci Technol 45(8):3252–3259CrossRefGoogle Scholar
  15. Hong K, Tokunaga S, Ishigami Y, Kajiuchi T (2000) Extraction of heavy metals from MSW incinerator fly ash using saponins. Chemosphere 41(3):345–352CrossRefGoogle Scholar
  16. Hong KJ, Tokunaga S, Kajiuchi T (2002) Evaluation of remediation process with plant-derived biosurfactant for recovery of heavy metals from contaminated soils. Chemosphere 49:379–387CrossRefGoogle Scholar
  17. Huang J, Ye J, Ma J, Gao J, Chen S, Wu X (2014) Triphenyltin biosorption, dephenylation pathway and cellular responses during triphenyltin biodegradation by Bacillus thuringiensis and tea saponin. Chem Eng J 249:167–173CrossRefGoogle Scholar
  18. Jackson A, Roy D, Breitenbeck G (1994) Transport of a bacterial suspension through a soil matrix using water and an anionic surfactant. Water Res 28(4):943–949CrossRefGoogle Scholar
  19. Kaczorek E, Smułek W, Zdarta A, Sawczuk A, Zgoła-Grześkowiak A (2016) Influence of saponins on the biodegradation of halogenated phenols. Ecotoxicol Environ Saf 131:127–134CrossRefGoogle Scholar
  20. Kevin JD, Peter TC, Roseanne MF (1995) Random walk calculations for bacterial migration in porous media. Biophys J 68:800–806CrossRefGoogle Scholar
  21. Lance J, Gerba CP (1984) Virus movement in soil during saturated and unsaturated flow. Appl Environ Microbiol 47(2):335–337Google Scholar
  22. Lappan RE, Fogler HS (1996) Reduction of porous media permeability from in situ leuconostoc mesenteroides growth and dextran production. Biotechbol Bioeng 50:6–15CrossRefGoogle Scholar
  23. Li Q, Logan BE (1999) Enhancing bacterial transport for bioaugmentation of aquifers using low ionic strength solutions and surfactants. Water Res 33:1090–1100CrossRefGoogle Scholar
  24. Liu ZF, Zeng GM, Wang J, Zhong H, Ding Y, Yuan XZ (2010) Effects of monorhamnolipid and Tween 80 on the degradation of phenol by Candida tropicalis. Process Biochem 45(5):805–809CrossRefGoogle Scholar
  25. Liu L, Gao B, Wu L, Morales VL, Yang L, Zhou Z, Wang H (2013) Deposition and transport of graphene oxide in saturated and unsaturated porous media. Chem Eng J 229:444–449CrossRefGoogle Scholar
  26. Liu YB, Qu D, Wen YJ, Ren HJ (2015) Low-temperature biodegradation of aniline by freely suspended and magnetic modified Pseudomonas migulae AN-1. Appl Microbiol Biotechnol 99(12):5317–5326CrossRefGoogle Scholar
  27. Mao X, Jiang R, Xiao W, Yu J (2015) Use of surfactants for the remediation of contaminated soils: a review. J Hazard Mater 285:419–435CrossRefGoogle Scholar
  28. Meinders J, Van der Mei H, Busscher H (1995) Deposition efficiency and reversibility of bacterial adhesion under flow. J Colloid Interface Sci 176(2):329–341CrossRefGoogle Scholar
  29. Michalsen MM, King AS, Rule RA, Fuller ME, Hatzinger PB, Condee CW, Crocker FH, Indest KJ, Jung CM, Istok JD (2016) Evaluation of biostimulation and bioaugmentation to stimulate hexahydro-1,3,5-trinitro-1,3,5,-triazine degradation in an aerobic groundwater aquifer. Environ Sci Technol 50(14):7625–7632CrossRefGoogle Scholar
  30. Mills AL, Herman JS, Hornberger GM, DeJesús TH (1994) Effect of solution ionic strength and iron coatings on mineral grains on the sorption of bacterial cells to quartz sand. Appl Environ Microbiol 60(9):3300–3306Google Scholar
  31. Oss CJV(1994) Polar or Lewis acid-base interactions. Interfacial forces in aqueous media, Marcel Dekker, New York .18–.46Google Scholar
  32. Pijanowska A, Kaczorek E, Olszanowski A (2007) Cell hydrophobicity of Pseudomonas spp. and Bacillus spp. bacteria and hydrocarbon biodegradation in the presence of Quillaya saponin. World J Microbiol Biotechnol 23(5):677–682CrossRefGoogle Scholar
  33. Qu D, Ren HJ, Zhou R, Zhao YS (2017) Visualisation study on Pseudomonas migulae AN-1 transport in saturated porous media. Water Res 122:329–336CrossRefGoogle Scholar
  34. Ripp S, Nivens DE, Werner C, Sayler GS (2001) Vertical transport of a field-released genetically engineered microorganism through soil. Soil Biol Biochem 33(12):1873–1877CrossRefGoogle Scholar
  35. Sanin SL, Sanin FD, Bryers JD (2003) Effect of starvation on the adhesive properties of xenobiotic degrading bacteria. Process Biochem 38:909–914CrossRefGoogle Scholar
  36. Shang J, Liu C, Wang Z (2013) Transport and retention of engineered nanoporous particles in porous media: effects of concentration and flow dynamics. Colloids Surf Physicochem Eng Aspects 417:89–98CrossRefGoogle Scholar
  37. Shoji Y, Igarashi T, Nomura H, Eitoku T, Katayama K (2012) Liposome solubilization induced by surfactant molecules in a microchip. Anal Sci 28(4):339–339CrossRefGoogle Scholar
  38. Sotirova AV, Spasova DI, Galabova DN, Karpenko E, Shulga A (2008) Rhamnolipid-biosurfactant permeabilizing effects on gram-positive and gram-negative bacterial strains. Curr Microbiol 56(6):639–644CrossRefGoogle Scholar
  39. Tang S, Bai J, Yin H, Ye J, Peng H, Liu Z, Dang Z (2014) Tea saponin enhanced biodegradation of decabromodiphenyl ether by Brevibacillus brevis. Chemosphere 114:255–261CrossRefGoogle Scholar
  40. Walshe GE, Pang L, Flury M, Close ME, Flintoft M (2010) Effects of pH, ionic strength, dissolved organic matter, and flow rate on the co-transport of MS2 bacteriophages with kaolinite in gravel aquifer media. Water Res 44(4):1255–1269CrossRefGoogle Scholar
  41. Zhang H, Ulrich AC, Liu Y (2015) Retention and transport of an anaerobic trichloroethene dechlorinating microbial culture in anaerobic porous media. Colloids Surf B Biointerfaces 130:110–118CrossRefGoogle Scholar
  42. Zhao YS, Li LL, Su Y, Qin CY (2014) Laboratory evaluation of the use of solvent extraction for separation of hydrophobic organic contaminants from surfactant solutions during surfactant-enhanced aquifer remediation. Sep Purif Technol 127:53–60CrossRefGoogle Scholar
  43. Zhao YS, Qu D, Zhou R, Ma Y, Wang H, Ren HJ (2016a) Bioaugmentation with GFP-tagged Pseudomonas migulae AN-1 in aniline-contaminated aquifer microcosms: cellular responses, survival and effect on indigenous bacterial community. J Microbiol Biotechnol 26(5):891–899CrossRefGoogle Scholar
  44. Zhao YS, Qu D, Zhou R, Yang S, Ren HJ (2016b) Efficacy of forming biofilms by Pseudomonas migulae AN-1 toward in situ bioremediation of aniline-contaminated aquifer by groundwater circulation wells. Environ Sci Pollut Res 23:11568–11573CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yongsheng Zhao
    • 1
  • Dan Qu
    • 1
    • 2
  • Rui Zhou
    • 1
  • Xinru Yang
    • 1
  • Wenbo Kong
    • 1
  • Hejun Ren
    • 1
  1. 1.Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and ResourcesJilin UniversityChangchunPeople’s Republic of China
  2. 2.Baohang Environment Company LimitedBeijingPeople’s Republic of China

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