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Environmental Science and Pollution Research

, Volume 25, Issue 24, pp 23712–23724 | Cite as

Nontuberculous mycobacteria in the environment of Hranice Abyss, the world’s deepest flooded cave (Hranice karst, Czech Republic)

  • Ivo Pavlik
  • Milan Gersl
  • Milan Bartos
  • Vit Ulmann
  • Petra Kaucka
  • Jan Caha
  • Adrian Unc
  • Dana Hubelova
  • Ondrej Konecny
  • Helena Modra
Research Article

Abstract

Nontuberculous mycobacteria (NTM) are widely distributed in the environment. On one hand, they are opportunistic pathogens for humans and animals, and on the other hand, they are effective in biodegradation of some persistent pollutants. Following the recently recorded large abundance of NTM in extreme geothermal environments, the aim of the study was to ascertain the occurrence of NTM in the extreme environment of the water zone of the Hranice Abyss (HA). The HA mineral water is acidic, with large concentrations of free CO2, and bacterial slimes creating characteristic mucilaginous formations. Both culture and molecular methods were used to compare the mycobacterial diversity across the linked but distinct ecosystems of HA and the adjacent Zbrašov Aragonite Caves (ZAC) with consideration of their pathogenic relevance. Six slowly growing NTM species (M. arupense, M. avium, M. florentinum, M. gordonae, M. intracellulare) and two rapidly growing NTM species (M. mucogenicum, M. sediminis) were identified in the water and in the dry zones at both sites. Proteobacteria were dominant in all the samples from both the HA and the ZAC. The bacterial microbiomes of the HA mineral water and HA slime were similar, but both differed from the microbiome in the ZAC mineral water. Actinobacteria, a phylum containing mycobacteria, was identified in all the samples at low proportional abundance. The majority of the detected NTM species belong among environmental opportunistic pathogens.

Keywords

Cave bacterial slimes Epidemiology Geomycobacteriology Environmental saprophytic mycobacteria Inorganic carbon 

Notes

Acknowledgements

This work was supported by the Czech Science Foundation (No. 16-13231S). The authors are indebted to Michal Guba, Jan Musil, and Martin Prachar (speleodivers, Czech Speleological Society 7-02 Hranicky Kras, Czech Republic) for underwater sample collection and to Barbora Simeckova (Cave Administration of the Czech Republic, Zbrašov Aragonite Caves, Hranice na Morave, Czech Republic) for her assistance during the sample collection. The authors also thank Vratislav Ouhrabka for creating the geological map of the HA and the ZAC.

Supplementary material

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Table S1 (DOCX 16 kb)
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Table S2 (DOCX 14 kb)
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Table S3 (DOCX 13 kb)
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Table S4 (DOCX 12 kb)

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Authors and Affiliations

  1. 1.Faculty of Regional Development and International StudiesMendel University in BrnoBrnoCzech Republic
  2. 2.Faculty of AgriSciencesMendel University in BrnoBrnoCzech Republic
  3. 3.Faculty of ScienceMasaryk UniversityBrnoCzech Republic
  4. 4.Public Health Institute OstravaOstravaCzech Republic
  5. 5.Faculty of PharmacyUniversity of Veterinary and Pharmaceutical Sciences BrnoBrnoCzech Republic
  6. 6.School of Science and the EnvironmentMemorial University of NewfoundlandCorner BrookCanada

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