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Intermittent micro-aeration control of methane emissions from an integrated vertical-flow constructed wetland during agricultural domestic wastewater treatment

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It is very important to control methane emissions to mitigate global warming. An intermittent micro-aeration control system was used to control methane emissions from an integrated vertical-flow constructed wetland (IVCW) to treat agricultural domestic wastewater pollution in this study. The optimized intermittent micro-aeration conditions were a 20-min aeration time and 340-min non-aeration time, 3.9 m3 h−1 aeration intensity, evenly distributed micro-aeration diffusers at the tank bottom, and an aeration period of every 6 h. Methane flux emission by intermittent micro-aeration was decreased by 60.7% under the optimized conditions. The average oxygen transfer efficiency was 26.73%. The control of CH4 emission from IVCWs was most strongly influenced by the intermittent micro-aeration diffuser distribution, followed by aeration intensity, aeration time, and water depth. Scaling up of IVCWs is feasible in rural areas by using intermittent micro-aeration control as a mitigation measure for methane gas emissions for climate change.

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  1. Ahmed T, Semmens MJ, Voss MA (2004) Oxygen transfer characteristics of hollow-fiber, composite membranes. Adv Environ Res 8(3–4):637–646

  2. ASCE (1997) Standard guidelines for in-process oxygen transfer testing. ASCE (American Society of Civil Engineering), New York

  3. ASCE (2006) ASCE Standard Measurement of Oxygen Transfer in Clean Water. ASCE (American Society of Civil Engineering), New York

  4. Bilgin M, Im Ek S, Tulun E (2014) Treatment of domestic wastewater using a lab-scale activated sludge/vertical flow subsurface constructed wetlands by using Cyperus alternifolius. Ecol Eng 70:362–365

  5. Button M, Nivala J, Weber KP, Aubron T, Müller RA (2015) Microbial community metabolic function in subsurface flow constructed wetlands of different designs. Ecol Eng 80:162–171

  6. Cattani M, Maccarana L, Rossi G, Tagliapietra F, Schiavon S, Bailoni L (2016) Dose-response and inclusion effects of pure natural extracts and synthetic compounds on in vitro methane production. Anim Feed Sci Technol 218:100–109

  7. Chiemchaisri C, Chiemchaisri W, Junsod J, Threedeach S, Wicranarachchi PN (2009) Leachate treatment and greenhouse gas emission in subsurface horizontal flow constructed wetland. Bioresour Technol 100(16):3808–3814

  8. Chowdhury T, Dick R (2013) Ecology of aerobic methanotrophs in controlling methane fluxes from wetlands. Appl Soil Ecol 65:8–22

  9. Cui L, Ouyang Y, Chen Y, Zhu X, Zhu W (2009) Removal of total nitrogen by Cyperus alternifolius from wastewaters in simulated vertical-flow constructed wetlands. Ecol Eng 35(8):1271–1274

  10. de la Varga D, Ruiz I, Álvarez JA, Soto M (2015) Methane and carbon dioxide emissions from constructed wetlands receiving anaerobically pretreated sewage. Sci Total Environ 538:824–833

  11. Dixon A, Simon M, Burkitt T (2003) Assessing the environmental impact of two options for small-scale wastewater treatment: comparing a reedbed and an aerated biological filter using a life cycle approach. Ecol Eng 20(4):297–308

  12. Fan J, Wang W, Zhang B, Guo Y, Ngo HH, Guo W, Zhang J, Wu H (2013a) Nitrogen removal in intermittently aerated vertical flow constructed wetlands: impact of influent COD/N ratios. Bioresour Technol 143:461–466

  13. Fan J, Zhang B, Zhang J, Ngo HH, Guo W, Liu F, Guo Y, Wu H (2013b) Intermittent aeration strategy to enhance organics and nitrogen removal in subsurface flow constructed wetlands. Bioresour Technol 141:117–122

  14. Fan J, Zhang J, Guo W, Liang S, Wu H (2016) Enhanced long-term organics and nitrogen removal and associated microbial community in intermittently aerated subsurface flow constructed wetlands. Bioresour Technol 214(Supplement C):871–875

  15. Feng Z, Li X, Lu C, Shen Z, Xu F, Chen Y (2012) Characterization of Pseudomonas mendocina LR capable of removing nitrogen from various nitrogen-contaminated water samples when cultivated with Cyperus alternifolius L. J Biosci Bioeng 114(2):182–187

  16. Foladori P, Ruaben J, Ortigara ARC (2013) Recirculation or artificial aeration in vertical flow constructed wetlands: a comparative study for treating high load wastewater. Bioresour Technol 149(Supplement C):398–405

  17. Fuchs VJ (2009) Nitrogen Removal and Sustainability in Vertical Flow Constructed Wetlands for Small Scale Wastewater Treatment. Michigan Technological University, Houghton

  18. Fuchs VJ, Mihelcic JR, Gierke JS (2011) Life cycle assessment of vertical and horizontal flow constructed wetlands for wastewater treatment considering nitrogen and carbon greenhouse gas emissions. Water Res 45(5):2073–2081

  19. Garnet KN, Megonigal JP, Litchfield C, Taylor GE Jr (2005) Physiological control of leaf methane emission from wetland plants. Aquat Bot 81(2):141–155

  20. Grünfeld S, Brix H (1999) Methanogenesis and methane emissions: effects of water table, substrate type and presence of Phragmites australis. Aquat Bot 64(1):63–75

  21. Hirasawa JS, Sarti A, Del AN, Varesche MB (2008) Application of molecular techniques to evaluate the methanogenic archaea and anaerobic bacteria in the presence of oxygen with different COD:sulfate ratios in a UASB reactor. Anaerobe 14(4):209–218

  22. Hospido A, Moreira MT, Fernández-Couto M, Feijoo G (2004) Environmental performance of a municipal wastewater treatment plant. Int J Life Cycle Assess 9(4):261–271

  23. Hu Y, He F, Ma L, Zhang Y, Wu Z (2016) Microbial nitrogen removal pathways in integrated vertical-flow constructed wetland systems. Bioresour Technol 207(Supplement C):339–345

  24. IPCC (2001) Atmospheric chemistry and greenhouse gases. In: Houghton JT et al (eds) Climate Change: The Scientific Basis. Cambridge University Press, Cambridge, pp 239–287 (Chapter 4)

  25. IPCC (2013) In: Stocker TF, Qin D, Plattner G-K, Tignor M, Allen SK, Boschung J, Nauels A, Xia Y, Bex V, Midgley PM (eds) Climate Change 2013: The Physical Science Basis. Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, Cambridge and New York 1535 pp

  26. IPCC (2014) 2013 supplement to the IPCC guidelines for National Greenhouse gas Inverntories: wetlands. IPCC, Switzerland

  27. Jia W, Zhang J, Wu J, Xie H, Zhang B (2010) Effect of intermittent operation on contaminant removal and plant growth in vertical flow constructed wetlands: a microcosm experiment. Desalination 262(1):202–208

  28. Johansson AE, Gustavsson AM, Oquist MG, Svensson BH (2004) Methane emissions from a constructed wetland treating wastewater-seasonal and spatial distribution and dependence on edaphic factors. Water Res 38:3960–3970

  29. Juottonen H, Galand PE, Tuittila ES, Laine J, Fritze H, Yrjala K (2005) Methanogen communities and Bacteria along an ecohydrological gradient in a northern raised bog complex. Environ Microbiol 7(10):1547–1557

  30. Kadlec RH, Wallace SD (2008) Treatmetn wetlands, 2nd edn. CRC Press, Boca Raton

  31. Keppler F, Hamilton JTG, Braß M, Röckmann T (2006) Methane emissions from terrestrial plants under aerobic conditions. Nature 439(7073):187–191

  32. Keppler F, Hamilton JTG, McRoberts WC, Vigano I, Braß M, Röckmann T (2008) Methoxyl groups of plant pectin as a precursor of atmospheric methane: evidence from deuterium labelling studies. New Phytologist 178(4):808–814

  33. Kiener A, Leisinger T (1983) Oxygen sensitivity of methanogenic Bacteria. Syst Appl Microbiol 4(3):305–312

  34. Koebsch F, Jurasinski G, Koch M, Hofmann J, Glatzel S (2015) Controls for multi-scale temporal variation in ecosystem methane exchange during the growing season of a permanently inundated fen. Agric For Meteorol 204:94–105

  35. Koelbener A, Ström L, Edwards PJ, Olde Venterink H (2010) Plant species from mesotrophic wetlands cause relatively high methane emissions from peat soil. Plant Soil 326(1):147–158

  36. Krasnits E, Friedler E, Sabbah I, Beliavski M, Tarre S, Green M (2009) Spatial distribution of major microbial groups in a well established constructed wetland treating municipal wastewater. Ecol Eng 35(7):1085–1089

  37. Kumar M, Singh R (2017) Performance evaluation of semi continuous vertical flow constructed wetlands (SC-VF-CWs) for municipal wastewater treatment. Bioresour Technol 232:321–330

  38. Lange M, Ahring BK (2001) A comprehensive study into the molecular methodology and molecular biology of methanogenic archaea. FEMS Microbiol Rev 25(5):553–571

  39. Leu S, Libra JA, Stenstrom MK (2010) Monitoring off-gas O2/CO2 to predict nitrification performance in activated sludge processes. Water Res 44(11):3434–3444

  40. Lewis WK, Whitman WG (1924) Principles of gas absorption. Ind Eng Chem 16(12):1215–1220

  41. Li J, Zhu L, Xu Y, Zhu B (2010) Oxygen transfer characteristics of hydrophilic treated polypropylene hollow fiber membranes for bubbleless aeration. J Membr Sci 362(1–2):47–57

  42. Li F, Lu L, Zheng X, Ngo HH, Liang S, Guo W, Zhang X (2014) Enhanced nitrogen removal in constructed wetlands: effects of dissolved oxygen and step-feeding. Bioresour Technol 169(Supplement C):395–402

  43. Liikanen A, Huttunen JT, Karjalainen SM, Heikkinen K, V Is Nen TS, Nyk Nen H, Martikainen PJ (2006) Temporal and seasonal changes in greenhouse gas emissions from a constructed wetland purifying peat mining runoff waters. Ecol Eng 26(3):241–251

  44. Liu T, Cao J (2007) Study on the isolation and growth condition of methanogen (in Chinese). Journal of Heilongjiang Hydraulic Engineering College (04):120–122

  45. Liu G, Ma L, Xing Z, College of Chemistry Engineering In Qingdao, China Training Center of Nomographic Chart (2002) Handbook of nomographic chart for physical property in chemistry engineering (in Chinese). China Chemistry Industry Press, Beijing, pp 245–286

  46. Liu D, Wu X, Chang J, Gu B, Min Y, Ge Y, Shi Y, Xue H, Peng C, Wu J (2012) Constructed wetlands as biofuel production systems. Nat Clim Chang 2(3):190–194

  47. Liu L, Zhao X, Zhao N, Shen Z, Wang M, Guo Y, Xu Y (2013) Effect of aeration modes and influent COD/N ratios on the nitrogen removal performance of vertical flow constructed wetland. Ecol Eng 57(Supplement C):10–16

  48. López D, Fuenzalida D, Vera I, Rojas K, Vidal G (2015) Relationship between the removal of organic matter and the production of methane in subsurface flow constructed wetlands designed for wastewater treatment. Ecol Eng 83:296–304

  49. Lundin M, Morrison GM (2002) A life cycle assessment based procedure for development of environmental sustainability indicators for urban water systems. Urban Water 4(2):145–152

  50. Machado AP, Urbano L, Brito AG, Janknecht P, Salas JJ, Nogueira R (2007) Life cycle assessment of wastewater treatment options for small and decentralized communities. Water Sci Technol 56(3):15–22

  51. Maltais-Landry G, Maranger R, Brisson J, Chazarenc F (2009) Greenhouse gas production and efficiency of planted and artificially aerated constructed wetlands. Environ Pollut 157(3):748–754

  52. Mander Ü, Lõhmus K, Teiter S, Mauring TN, Nurk K, Augustin J (2008) Gaseous fluxes in the nitrogen and carbon budgets of subsurface flow constructed wetlands. Sci Total Environ 404(2–3):343–353

  53. Mander Ü, Dotro G, Ebie Y, Towprayoon S, Chiemchaisri C, Nogueira SF, Jamsranjav B, Kasak K, Truu J, Tournebize J, Mitsch WJ (2014) Greenhouse gas emission in constructed wetlands for wastewater treatment: a review. Ecol Eng 66:19–35

  54. Mander Ü, Maddison M, Soosaar K, Koger H, Teemusk A, Truu J, Well R, Sebilo M (2015) The impact of a pulsing water table on wastewater purification and greenhouse gas emission in a horizontal subsurface flow constructed wetland. Ecol Eng 80:69–78

  55. Mata-Alvarez J, Macé S, Llabrés P (2000) Anaerobic digestion of organic solid wastes. An overview of research achievements and perspectives. Bioresour Technol 74(1):3–16

  56. Mathrani IM, Boone DR, Mah RA, Fox GE, Lau PP (1988) Methanohalophilus zhilinae sp. nov., an alkaliphilic, halophilic, methylotrophic methanogen. Int J Syst Evol Microbiol 38(2):139–142

  57. Maucieri C, Barbera AC, Vymazal J, Borin M (2017) A review on the main affecting factors of greenhouse gases emission in constructed wetlands. Agric For Meteorol 236:175–193

  58. McPhillips L, Walter MT (2015) Hydrologic conditions drive denitrification and greenhouse gas emissions in stormwater detention basins. Ecol Eng 85:67–75

  59. Medvedeff CA, Bridgham SD, Pfeifer-Meister L, Keller JK (2015) Can Sphagnum leachate chemistry explain differences in anaerobic decomposition in peatlands? Soil Biol Biochem 86:34–41

  60. Metcalf & Eddy Inc, Tchobanoglous G, Burton FL, Stensel HD (2002) Wastewater engineering. Treatmentand reuse, 4th edn. McGraw-Hill, New York

  61. Molle P, Prost-Boucle S, Lienard A (2008) Potential for total nitrogen removal by combining vertical flow and horizontal flow constructed wetlands: a full-scale experiment study. Ecol Eng 34(1):23–29

  62. Mueller JA, Boyle WC, Pöpel HJ (2002) Aeration: principles and practice. CRC Press, Boca Raton

  63. Murphy C, Rajabzadeh AR, Weber KP, Nivala J, Wallace SD, Cooper DJ (2016) Nitrification cessation and recovery in an aerated saturated vertical subsurface flow treatment wetland: field studies and microscale biofilm modeling. Bioresour Technol 209:125–132

  64. Nivala J, Wallace S, Headley T, Kassa K, Brix H, van Afferden M, Müller R (2013) Oxygen transfer and consumption in subsurface flow treatment wetlands. Ecol Eng 61(Part B):544–554

  65. Ortiz-Llorente MJ, Alvarez-Cobelas M (2012) Comparison of biogenic methane emissions from unmanaged estuaries, lakes, oceans, rivers and wetlands. Atmos Environ 59:328–337

  66. Pan T, Zhu X, Ye Y (2011) Estimate of life-cycle greenhouse gas emissions from a vertical subsurface flow constructed wetland and conventional wastewater treatment plants: a case study in China. Ecol Eng 37(2):248–254

  67. Pennock D, Yates T, Bedard-Haughn A, Phipps K, Farrell R, McDougal R (2010) Landscape controls on N2O and CH4 emissions from freshwater mineral soil wetlands of the Canadian prairie pothole region. Geoderma 155(3–4):308–319

  68. Pittoors E, Guo Y, Van Hulle SWH (2014) Oxygen transfer model development based on activated sludge and clean water in diffused aerated cylindrical tanks. Chem Eng J 243:51–59

  69. Puyuelo B, Gea T, Sánchez A (2014) GHG emissions during the high-rate production of compost using standard and advanced aeration strategies. Chemosphere 109:64–70

  70. Reay D, Smith P, Amstel AV (2010) Methane and climate change. Earthscan, Washington, DC

  71. Rosso D, Stenstrom MK, Larson LE (2008) Aeration of large-scale municipal wastewater treatment plants: state of the art. Water Sci Technol 57(7):973–978

  72. Rychlik JL, May T (2000) The effect of a methanogen, Methanobrevibacter smithii, on the growth rate, organic acid production, and specific ATP activity of three predominant ruminal cellulolytic bacteria. Curr Microbiol 40(3):176–180

  73. Smet E, Van Langenhove H, De Bo I (1999) The emission of volatile compounds during the aerobic and the combined anaerobic/aerobic composting of biowaste. Atmos Environ 33:1295–1303

  74. Soda S, Hamada T, Yamaoka Y, Ike M, Nakazato H, Saeki Y, Kasamatsu T, Sakurai Y (2012) Constructed wetlands for advanced treatment of wastewater with a complex matrix from a metal-processing plant: bioconcentration and translocation factors of various metals in Acorus gramineus and Cyperus alternifolius. Ecol Eng 39:63–70

  75. Søvik AK, KløVe B (2007) Emission of N2O and CH4 from a constructed wetland in southeastern Norway. Sci Total Environ 380(1–3):28–37

  76. Sun L, Song C, Miao Y, Qiao T, Gong C (2013) Temporal and spatial variability of methane emissions in a northern temperate marsh. Atmos Environ 81:356–363

  77. Sun J, Liang P, Yan X, Zuo K, Xiao K, Xia J, Qiu Y, Wu Q, Wu S, Huang X, Qi M, Wen X (2016) Reducing aeration energy consumption in a large-scale membrane bioreactor: process simulation and engineering application. Water Res 93:205–213

  78. Syron E, Semmens MJ, Casey E (2015) Performance analysis of a pilot-scale membrane aerated biofilm reactor for the treatment of landfill leachate. Chem Eng J 273:120–129

  79. Taricska JR, Huang JYC, Chen P, Hung YT, Zou SW (2009) In: Wang LK, Pereira NC, Hung YT, Shammas NK (eds) Biological Treatment Processes. The Humana Press, Totowa

  80. Tuttolomondo T, Licata M, Leto C, Leone R, La Bella S (2015) Effect of plant species on water balance in a pilot-scale horizontal subsurface flow constructed wetland planted with Arundo donax L. and Cyperus alternifolius L. – two-year tests in a Mediterranean environment in the West of Sicily (Italy). Ecol Eng 74:79–92

  81. Uggetti E, Hughes-Riley T, Morris RH, Newton MI, Trabi CL, Hawes P, Puigagut J, Garcia J (2016) Intermittent aeration to improve wastewater treatment efficiency in pilot-scale constructed wetland. Sci Total Environ 559:212–217

  82. US-EPA (2003) National menu of best management practices for storm water phase II. Office of Water, Washington, DC

  83. Vigano I, Van Weelden H, Holzinger R, Keppler F, Röckmann T (2008) Effect of UV radiation and temperature on the emission of methane from plant biomass and structural components. Biogeosciences 5(1):243–270

  84. Vymazal J (2011) Constructed wetlands for wastewater treatment: five decades of experience. Environ Sci Technol 45(1):61–69

  85. Waddington JM, Roulet NT, Swanson RV (1996) Water table control of CH4 emission enhancement by vascular plants in boreal peatlands. J Geophys Res Atmos 101(D17):22775–22785

  86. Wang Y, Inamori R, Kong H, Xu K, Inamori Y, Kondo T, Zhang J (2008) Influence of plant species and wastewater strength on constructed wetland methane emissions and associated microbial populations. Ecol Eng 32(1):22–29

  87. Willis J (2004) Data Analysis and Presentation Skills: An Introduction for the Life and Medical Sciences. Wiley, Chichester

  88. Wu ZB (2008) Integrated vertical-flow constructed wetland. Science Press, Beijing

  89. Wu H, Fan J, Zhang J, Ngo HH, Guo W, Liang S, Hu Z, Liu H (2015) Strategies and techniques to enhance constructed wetland performance for sustainable wastewater treatment. Environ Sci Pollut R 22(19):14637–14650

  90. Wu H, Lin L, Zhang J, Guo W, Liang S, Liu H (2016) Purification ability and carbon dioxide flux from surface flow constructed wetlands treating sewage treatment plant effluent. Bioresour Technol 219:768–772

  91. Wu H, Fan J, Zhang J, Ngo HH, Guo W (2018) Large-scale multi-stage constructed wetlands for secondary effluents treatment in northern China: carbon dynamics. Environ Pollut 233:933–942

  92. Xiao K, Xu Y, Liang S, Lei T, Sun J, Wen X, Zhang H, Chen C, Huang X (2014) Engineering application of membrane bioreactor for wastewater treatment in China: current state and future prospect. Front Environ Sci Eng China 8(6):805–819

  93. Xu D, Wang L, Li H, Li Y, Howard A, Guan Y, Li J, Xu H (2015) The forms and bioavailability of phosphorus in integrated vertical flow constructed wetland with earthworms and different substrates. Chemosphere 134:492–498

  94. Yan Q, Gao X, Guo J, Zhu Z, Feng G (2016) Insights into the molecular mechanism of the responses for Cyperus alternifolius to PhACs stress in constructed wetlands. Chemosphere 164:278–289

  95. Yang G (2008) The Effect of Low Temperature on Biogas Production Capability and Isolation of Methanogenic Bacteria (in Chinese). Northwest A & F University, Yangling, p 51

  96. Yang J, Liu J, Hu X, Li X, Wang Y, Li H (2013) Effect of water table level on CO2, CH4 and N2O emissions in a freshwater marsh of Northeast China. Soil Biol Biochem 61(0):52–60

  97. Yang P, He Q, Huang J, Tong C (2015) Fluxes of greenhouse gases at two different aquaculture ponds in the coastal zone of southeastern China. Atmos Environ 115:269–277

  98. Ye R, Espe MB, Linquist B, Parikh SJ, Doane TA, Horwath WR (2016) A soil carbon proxy to predict CH4 and N2O emissions from rewetted agricultural peatlands. Agric Ecosyst Environ 220:64–75

  99. Zhang D, Gersberg RM, Keat TS (2009) Constructed wetlands in China. Ecol Eng 35(10):1367–1378

  100. Zhang L, Zhang L, Liu Y, Shen Y, Liu H, Xiong Y (2010) Effect of limited artificial aeration on constructed wetland treatment of domestic wastewater. Desalination 250(3):915–920

  101. Zhao Z, Chang J, Han W, Wang M, Ma D, Du Y, Qu Z, Chang SX, Ge Y (2016) Effects of plant diversity and sand particle size on methane emission and nitrogen removal in microcosms of constructed wetlands. Ecol Eng 95:390–398

  102. Zhu X, Song C, Guo Y, Sun X, Zhang X, Miao Y (2014) Methane emissions from temperate herbaceous peatland in the Sanjiang plain of Northeast China. Atmos Environ 92:478–483

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This work was supported by the National Natural Science Foundation of China (51278318), the Chengdu Science & Technology Bureau (2015-HM01-00325-SF), the Science and Technology Department of Sichuan Province (18ZDYF3209), and the State Key Laboratory of Hydraulics and Mountain River Engineering in China (SKHL1716).

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Correspondence to Hongbing Luo.

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Research highlights

• Optimal intermittent micro-aeration control of CH4 emission from integrated vertical flow constructed wetlands (IVCWs): 20 min aeration time, 340 min interruption time, 3.9 m3 h−1 aeration intensity, and every 6 h as an aeration operation period.

• Controlled condition on CH4 emission in IVCWs were the order of the intermittent micro-aeration diffuser distributions at the tank bottom > the aeration intensity (m3 h−1) > the aeration time (minutes) > the water tables.

• Averagely distributed micro-aeration diffusers was less CH4 emission than the middle intensively distributed micro-aeration diffusers at the tank bottom.

Responsible editor: Philippe Garrigues

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Liu, X., Zhang, K., Fan, L. et al. Intermittent micro-aeration control of methane emissions from an integrated vertical-flow constructed wetland during agricultural domestic wastewater treatment. Environ Sci Pollut Res 25, 24426–24444 (2018).

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  • Intermittent micro-aeration
  • Integrated vertical-flow constructed wetland
  • Control of methane emissions
  • Oxygen transfer efficiency
  • Cyperus alternifolius L.