Environmental Science and Pollution Research

, Volume 25, Issue 20, pp 19887–19897 | Cite as

Screening optimal substrates from Erhai lakeside for Ottelia acuminata (Gagnep.) Dandy, an endangered submerged macrophyte in China

  • Shuqin Chen
  • Zhaosheng Chu
  • Yunqiao Zhou
  • Qifeng Li
  • Tieyu WangEmail author
Research Article


Because of the unstable hydrodynamic conditions in the wild, the endangered aquatic plant should be cultivated first in constructed wetlands for the protection and expansion of germplasm resources. Ottelia acuminata (Gagnep.) Dandy has become extinct in Erhai Lake, Yunnan province, China. In order to optimize substrates for this species to artificial cultivation, the native substrate (sandy soils) and the other three representative ones (red paddy soils, alluvial paddy soils, and purple paddy soils) collected from Erhai lakeside were applied to cultivate O. acuminata for 50 days. Multi indicators, such as antioxidant enzymes activity, malondialdehyde and chlorophyll-α concentration, and relative growth rate of O. acuminata, were discussed and statistically analyzed to classify the substrates. The results suggested that even disregarding the physiology significance of these indicators, hierarchical clustering analysis had high efficiency on optimizing substrates. Although various single indexes suggested different optimal substrates for macrophyte growth, red paddy soil was never excluded out the optimal substrate classes. Further study is needed to assess the substrates optimization functionalities of these indicators. This study offers amounts of physiology data and an effective method to optimize substrates of O. acuminata. It is helpful for environmental scientists and ecological engineers to conduct the similar study on endangered species.


Ottelia acuminata (Gagnep.) Dandy Substrates Optimizing Erhai Lake Hierarchical clustering analysis Antioxidant enzyme 


Funding information

This research was supported by the National Key R&D Program of China (Grant No. 2017YFC0505702), the National Natural Science Foundation of China (Grant No. 41571478), the National Water Pollution Control and Treatment Science and Technology Major Project (Grant No. 2015ZX07203-005), the Anhui Provincial Top College Teacher Studying Visit Program (Grant No. gxfx2017057), the Yangtze River Ecology Investigation Project (Grant No. 043-140004), and the Doctor Scientific Research Foundation of Anqing Normal University (Grant No. 150001012). We would also like to acknowledge support from the Youth Innovation Promotion Association of the Chinese Academy of Sciences.

Supplementary material

11356_2018_2200_MOESM1_ESM.doc (1.6 mb)
ESM 1 (DOC 1648kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Shuqin Chen
    • 1
    • 2
    • 3
  • Zhaosheng Chu
    • 4
  • Yunqiao Zhou
    • 1
    • 3
  • Qifeng Li
    • 1
    • 3
  • Tieyu Wang
    • 1
    • 3
    Email author
  1. 1.State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijingChina
  2. 2.College of Resource and EnvironmentAnqing Normal UniversityAnhuiChina
  3. 3.University of Chinese Academy of SciencesBeijingChina
  4. 4.China Research Academy of Environment and ScienceBeijingChina

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