Environmental Science and Pollution Research

, Volume 25, Issue 20, pp 19436–19445 | Cite as

In situ pilot test for bioremediation of energetic compound-contaminated soil at a former military demolition range site

  • Louis B. Jugnia
  • Dominic Manno
  • Karine Drouin
  • Meghan Hendry
Research Article


Bioremediation was performed in situ at a former military range site to assess the performance of native bacteria in degrading hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and 2,4-dinitrotoluene (2,4-DNT). The fate of these pollutants in soil and soil pore water was investigated as influenced by waste glycerol amendment to the soil. Following waste glycerol application, there was an accumulation of organic carbon that promoted microbial activity, converting organic carbon into acetate and propionate, which are intermediate compounds in anaerobic processes. This augmentation of anaerobic activity strongly correlated to a noticeable reduction in RDX concentrations in the amended soil. Changes in concentrations of RDX in pore water were similar to those observed in the soil suggesting that RDX leaching from the soil matrix, and treatment with waste glycerol, contributed to the enhanced removal of RDX from the water and soil. This was not the case with 2,4-DNT, which was neither found in pore water nor affected by the waste glycerol treatment. Results from saturated conditions and Synthetic Precipitation Leaching Procedure testing, to investigate the environmental fate of 2,4-DNT, indicated that 2,4-DNT found on site was relatively inert and was likely to remain in its current state on the site.


In situ Pilot test Biodegradation Carbon source Waste glycerol RDX 2,4-DNT 



We thank the four CDSB Environment Services of the Canadian Department of National Defense at Garrison Petawawa for funding of this work. We thank N. Kemka for assistance with field work, A. Halasz for technical support, and L. Paquet and A. Corriveau for the analytical support.


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Copyright information

© Crown 2018

Authors and Affiliations

  • Louis B. Jugnia
    • 1
  • Dominic Manno
    • 1
  • Karine Drouin
    • 1
  • Meghan Hendry
    • 2
  1. 1.Energy, Mining and Environment Research CentreNational Research Council CanadaMontrealCanada
  2. 2.National DefenceGarrison PetawawaStn Main PetawawaCanada

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