Environmental Science and Pollution Research

, Volume 25, Issue 18, pp 18109–18120 | Cite as

Intestinal microbiome of broiler chickens after use of nanoparticles and metal salts

  • Еlena Yausheva
  • Sergey Miroshnikov
  • Еlena Sizova
Research Article


The research included the study of influence of ultrafine particle preparations (nanoparticles of copper, zinc, iron, CuZn alloy) and metal salts (iron pyrophosphate, copper asparginate, zinc asparginate) on the composition of cecal microbiota of broiler chickens. Before adding the studied nanoparticles and metal salts to the diet, cecal microbiota of broiler chickens was represented by 76% Firmicutes taxon and 16% Bacteroidetes. Numerous among them were the bacteria of the taxa Anaerotruncus spp., Lactobacillus spp., Blautia spp., Alistipes spp., and Bacteroides spp.; they constituted 18, 17, 11, and 6%, respectively. A peculiarity of action of the most analyzed metals in nanoform and in the form of salts was a decrease in the number of phylum Firmicutes bacteria and an increase in the number of microorganisms of the phylum Bacteroidetes. The number of bacteria belonging to the families Ruminococcaceae (III, IV, V, VII, and VIII groups), Bacteroidaceae (in all experimental groups), and Lachnospiraceae (I, IV, V, and VII groups) was registered within the taxa of Firmicutes and Bacteroidetes. At the same time, in some experimental groups, the number of bacteria of the family Lachnospiraceae (II, III, and VIII) decreased in the intestine. The data obtained can be used to assess the possibility of using metal nanoparticles in the poultry diet, as a micronutrient preparation, to correct dysbiosis and to improve the utilization of fodder energy.


Nanoparticles Copper Iron Zinc Microbiota Metagenomic sequencing 


Funding information

The work was carried out with the financial support of the Russian Science Foundation (project No. 14-16-00060 P).

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Еlena Yausheva
    • 1
  • Sergey Miroshnikov
    • 1
  • Еlena Sizova
    • 1
    • 2
  1. 1.State Educational Institution All-Russian Research Institute of Beef Cattle BreedingOrenburgRussia
  2. 2.Orenburg State UniversityOrenburgRussia

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