Biodegradation of kraft lignin by newly isolated Klebsiella pneumoniae, Pseudomonas putida, and Ochrobactrum tritici strains
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Bacterial systems have drawn an increasing amount of attention on lignin valorization due to their rapid growth and powerful environmental adaptability. In this study, Klebsiella pneumoniae NX-1, Pseudomonas putida NX-1, and Ochrobactrum tritici NX-1 with ligninolytic potential were isolated from leaf mold samples. Their ligninolytic capabilities were determined by measuring (1) the cell growth on kraft lignin as the sole carbon source, (2) the decolorization of kraft lignin and lignin-mimicking dyes, (3) the micro-morphology changes and transformations of chemical groups in kraft lignin, and (4) the ligninolytic enzyme activities of these three isolates. To the best of our knowledge, this is the first report that Ochrobactrum tritici species can depolymerize and metabolize lignin. Moreover, laccase, lignin peroxidase, and Mn-peroxidase showed high activities in P. putida NX-1. Due to their excellent ligninolytic capabilities, these three bacteria are important supplements to ligninolytic bacteria library and could be valuable in lignin valorization.
KeywordsLignin Biodegradation Klebsiella pneumoniae Pseudomonas putida Ochrobactrum tritici Ligninolytic enzymes
This work was supported by the National Key R&D Program of China (grant number 2016YFE0105400), National Natural Science Foundation of China (grant number 21606132), Natural Science Foundation of Jiangsu Province (grant numbers BK20160823 and BK20170829), and Fundamental Research Funds for the Central Universities (grant numbers 30916011202 and 30917011307).
- Cohen MS, Gabriele PD (1982) Degradation of coal by the fungi Polyporus versicolor and Poria monticola. Appl Microbiol Biotechnol 44:23–27Google Scholar
- Holladay JE, White JF, Bozell JJ, Johnson D (2007) Top value-added chemicals from biomass—volume II. Results of screening for potential candidates from biorefinery lignin (No. PNNL-16983). Pacific Northwest National Lab.(PNNL), Richland, WA (United States); National Renewable Energy Laboratory (NREL), Golden, CO (United States)Google Scholar
- Humbird D, Davis R, Tao L, Kinchin C, Hsu D, Aden A (2011) Process design and economics for biochemical conversion of lignocellulosic biomass to ethanol. NREL technical report, NREL/TP-5100-47764: 1–114Google Scholar
- Mathews SL, Grunden AM, Pawlak J (2016) Degradation of lignocellulose and lignin by Paenibacillus glucanolyticus. Int Biodeter Biodegr 110:79–86Google Scholar
- Morii H, Nakamiya K, Kinoshita S (1995) Isolation of a lignin-decolorizing bacterium. J Biosci Bioeng 80:296–299Google Scholar
- Ramachandra M, Crawford DL, Hertel G (1988) Characterization of an extracellular lignin peroxidase of the lignocellulolytic actinomycete Streptomyces viridosporus. Appl Environ Microbiol 54(12):3057–3063Google Scholar
- Shields-Menard SA, AmirSadeghi M, Green M, Womack E, Sparks DL, Blake J, Edelmann M, Ding X, Sukhbaatar B, Hernandez R, Donaldson JR, French TR (2017) The effects of model aromatic lignin compounds on growth and lipid accumulation of Rhodococcus rhodochrous. Int Biodeterior Biodegrad 121:79–90CrossRefGoogle Scholar